荧光光度法测定钇含量
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摘要
在pH 9.2的硼砂-盐酸缓冲溶液中,在一定量的十六烷基三甲基溴化铵存在下,鞣花酸与钇(Ⅲ)反应使其荧光淬灭,据此提出了测定痕量钇(Ⅲ)的荧光光度法。通过测定样品溶液和空白溶液在发射波长454nm处的荧光强度,计算得反应液荧光强度淬灭程度ΔIf,其荧光强度的淬灭程度与钇(Ⅲ)的浓度在2.0×10-6～8.0×10-5 mol·L-1范围内呈线性关系,方法的检出限(3S/N)为2.63×10-7 mol·L-1。方法的回收率在99.0%～99.5%之间。并利用共振散射光谱法和紫外可见光谱研究体系的作用机理。
In a borax-HCl buffer medium of pH 9.2 and in the presence of CTMAB,fluorescence quenching effect was observed when ellagic acid was reacted with Y(Ⅲ).Based on this fact,a method of fluorospectrophotometry for the determination of Y(Ⅲ) was proposed.The magnitude of decrease in fluorescence intensity(ΔIf) of the reaction solution were measured at the wavelength of 454 nm by taking the difference between emission fluorescence intensity of the blank solution and the sample solution.Linear relationship between values of ΔIf and concentration of Y(Ⅲ) was found in the range of 2.0×10-6-8.0×10-5mol·L-1,with detection limit(3S/N) of 2.63×10-7mol·L-1.Values of recovery found were in the range of 99.0%-99.5%.Machanism of the action system was studied by resonance scattering spectrometry and UV-visible spectrometry.
In a borax-HCl buffer medium of pH 9.2 and in the presence of CTMAB,fluorescence quenching effect was observed when ellagic acid was reacted with Y(Ⅲ).Based on this fact,a method of fluorospectrophotometry for the determination of Y(Ⅲ) was proposed.The magnitude of decrease in fluorescence intensity(ΔIf) of the reaction solution were measured at the wavelength of 454 nm by taking the difference between emission fluorescence intensity of the blank solution and the sample solution.Linear relationship between values of ΔIf and concentration of Y(Ⅲ) was found in the range of 2.0×10-6-8.0×10-5mol·L-1,with detection limit(3S/N) of 2.63×10-7mol·L-1.Values of recovery found were in the range of 99.0%-99.5%.Machanism of the action system was studied by resonance scattering spectrometry and UV-visible spectrometry.
引文
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[14]HASEGAWA M,TERAUCHI M,KIKUCHI Y,etal.Deprotonation processes of ellagic acid in solutionand solid states[J].Monatsh Chem,2003,134:811-821.
[15]PRESS R E,HARDCASTLE D.Some physico-chemical properties of ellagic acid[J].J Appl Chem,1969,19:247-251.
[16]OTTO S W,PETRA H.The fluorescence of ellagicacid and broax complex[J].Monatshefte Fur Chemie,1986,117:369-374.
[2]王红.鞣花酸开发前景看好[J].应用科技,2000,27(2):36-37.
[3]王黎,李峰.石榴皮鞣花酸类色素的提取和性能测定[J].信阳农业高等专科学校学报,2006,16(3):133-135.
[4]ROMMEL A,WROLSTAD R E.Ellagic acid contentof red raspberry juice as influenced by cultivar,pro-cessing,and environmental factors[J].J Agric FoodChem,1993,41(11):1951-1960.
[5]TEEL R W.Ellagic acid binding to DNA as a possiblemechanism for its antimutagenic and anticarcinogenicaction[J].Cancer Letters,1986,30(3):329-336.
[6]李庆,姚开,谭敏.新型天然抗氧化剂-鞣花酸[J].四川食品与发酵,2001,37(4):10-18.
[7]赵一兵,王冬媛,许金钩.一类自猝灭的新型荧光探针研究[J].厦门大学学报:自然科学版,1997,36(4):594-598.
[8]杨景和,朱贵云.稀土元素的共发光效应[J].山东大学学报,1986,21(4):133-139.
[9]朱勇.光致发光稀土-高分子配合物的研究进展[J].中北大学学报,2007,28(6):532-533.
[10]吕元琦,盛勇,周连文.茴香调味品中稀土元素的电感耦合等离子体质谱分析[J].化学工程师,2006,124(1):43-44.
[11]李继东,伍星,郑永章.微柱分离-ICP-MS测定高纯氧化钆14种稀土杂质[J].分析测试学报,2005,24(4):32-34.
[12]张翼明,郝冬梅,张志刚.ICP-MS法测定金属镝及其氧化物中14种稀土杂质[J].稀土,2006,27(3):69-71.
[13]李继东,伍星,郑永章.微柱分离-ICP-MS测定高纯氧化钐中稀土杂质[J].中国稀土学报,2005,23(1):109-112.
[14]HASEGAWA M,TERAUCHI M,KIKUCHI Y,etal.Deprotonation processes of ellagic acid in solutionand solid states[J].Monatsh Chem,2003,134:811-821.
[15]PRESS R E,HARDCASTLE D.Some physico-chemical properties of ellagic acid[J].J Appl Chem,1969,19:247-251.
[16]OTTO S W,PETRA H.The fluorescence of ellagicacid and broax complex[J].Monatshefte Fur Chemie,1986,117:369-374.