梅花鹿致病性源坏死梭杆菌的分离与鉴定
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摘要
为确定湖北省恩施自治州某鹿场致患病鹿蹄腐烂的病原菌,本研究于患病梅花鹿的前蹄病健结合处采集病料并对其进行细菌分离培养,最终分离到一株厌氧菌。对该细菌进行了形态观察、生化试验、16S rRNA基因序列测定、细菌生化鉴定仪鉴定、药物敏感性试验以及小鼠致病力试验等。结果显示该细菌在显微镜下呈革兰氏阴性、丝状、长短不一的杆菌。16S rRNA基因序列测定结果显示该分离菌株与多株坏死梭杆菌参考株的同源性均为99%,将其命名为HNFnf;经Vitek细菌鉴定仪鉴定该细菌为坏死梭杆菌;药敏试验结果显示,该菌对青霉素、头孢类等中度敏感,对阿米卡星、链霉素、庆大霉素耐药。本研究为临床治疗由坏死梭杆菌引起的反刍动物"腐蹄病"与"肝脓肿"提供参考依据。
The present study was performed to find the pathogen caused the rotted hoff of sika deer at the farm located in Enshi state, Hubei province of China. The anaerobe was isolated from rotted hoof samples of the sika deer which were collected and cultured in BHI medium. The result showed the isolated bacteria was identified as Fusobacterium necrophorum by morphologic observation, biochemical test, 16S rRNA sequencing, bacterial biochemical identification, druy susceptibility test and pathogenicity experiment. The results indicated the isolate named HNFnf was gram-negative bacilli, filaments of varying lengths under microscope, and showed 99% homology with F.necrophorun through 16S rRNA gene sequencing. It was concluded that the HNFnf belonged to F.necrophorum faculty determinated by Vitek Bio-chemical identification instrument. The isolate was intermediate susceptible to penicillin and cephalosporins, but resistant to amikacin, streptomucin and gentamycin by the druy susceptibility test. The study provides reference for clinical treatment of "footrot disease" and "liver abscess" infected with F.necrophorum in ruminal animals.
The present study was performed to find the pathogen caused the rotted hoff of sika deer at the farm located in Enshi state, Hubei province of China. The anaerobe was isolated from rotted hoof samples of the sika deer which were collected and cultured in BHI medium. The result showed the isolated bacteria was identified as Fusobacterium necrophorum by morphologic observation, biochemical test, 16S rRNA sequencing, bacterial biochemical identification, druy susceptibility test and pathogenicity experiment. The results indicated the isolate named HNFnf was gram-negative bacilli, filaments of varying lengths under microscope, and showed 99% homology with F.necrophorun through 16S rRNA gene sequencing. It was concluded that the HNFnf belonged to F.necrophorum faculty determinated by Vitek Bio-chemical identification instrument. The isolate was intermediate susceptible to penicillin and cephalosporins, but resistant to amikacin, streptomucin and gentamycin by the druy susceptibility test. The study provides reference for clinical treatment of "footrot disease" and "liver abscess" infected with F.necrophorum in ruminal animals.
引文
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[6]Alfreijat M.A case of Lemierre's Syndrome with a brief literature review[J].J Infect Public Health,2016,9(5):681-683.
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[9]姚志利.致病性坏死梭杆菌分子检测与基因分型[D].北京:中国农业科学院,2010.
[10]Shanthalingam S,Narayanan S,Batra S A,et al.Fusobacterium necrophorum in North American Bighorn Sheep(Ovis canadensis)pneumonia[J].J Wildl Dis,2016,52(3):616-620.
[11]Brooks J W,Kumar A,Narayanan S,et al.Characterization of Fusobacterium isolates from the respiratory tract of white-tailed deer(Odocoileus virginianus)[J].J Vet Diagn Invest,2014,26(2):213-220.
[2]Farooq S,Wani S A,Hassan M N,et al.The detection of Dichelobacter nodosus and Fusobacterium necrophorum from ovine footrot in Kashmir,India[J].Anaerobe,2015,35(Pt B):41-43.
[3]Bennett G N,Hickford J G.Ovine footrot:new approaches to an old disease[J].Vet Microbiol,2011,148(1):1-7.
[4]Zhou H,Dobbinson S,Hickford J G.Fusobacterium necrophorum variants present on the hooves of lame pigs[J].Vet Microbiol,2010,141(3-4):390.
[5]Kj覸rulff A M,Thomsen M K,Ovesen T,et al.Clinical and biochemical characteristics of patients with Fusobacterium necrophorum-positive acute tonsillitis[J].Eur Arch Otorhinolaryngol,2015,272(6):1457-1463.
[6]Alfreijat M.A case of Lemierre's Syndrome with a brief literature review[J].J Infect Public Health,2016,9(5):681-683.
[7]Haddad N,Morris T,Dhillon R,et al.Unusual neurological presentation of Fusobacterium necrophorum disease[J].BMJ Case Rep,2016,2016:bcr2015210710.
[8]Hannoodi F,Sabbagh H,Kulairi Z,et al.A rare case of Fusobacterium necrophorum liver abscesses[J].Clini Pract,2017,7(1):928.
[9]姚志利.致病性坏死梭杆菌分子检测与基因分型[D].北京:中国农业科学院,2010.
[10]Shanthalingam S,Narayanan S,Batra S A,et al.Fusobacterium necrophorum in North American Bighorn Sheep(Ovis canadensis)pneumonia[J].J Wildl Dis,2016,52(3):616-620.
[11]Brooks J W,Kumar A,Narayanan S,et al.Characterization of Fusobacterium isolates from the respiratory tract of white-tailed deer(Odocoileus virginianus)[J].J Vet Diagn Invest,2014,26(2):213-220.