二乙烯亚胺对水貂肠炎细小病毒灭活效果的研究
|
摘要
为研究二乙烯亚胺(BEI)对水貂肠炎细小病毒的灭活效果,采用BEI终浓度为0.003mol/L、0.002mol/L、0.001mol/L、0.0005mol/L,灭活温度为30℃,灭活时间为24h、48h、72h条件下,对水貂肠炎细小病毒进行灭活试验。通过F81细胞传代观察细胞病变和血凝试验检测灭活效果;用水貂检验BEI灭活水貂肠炎细小病毒所制疫苗的安全性,通过检测接种水貂的血凝抑制抗体,评价BEI灭活细小病毒所制疫苗的免疫效果,同时与甲醛灭活的疫苗进行免疫效果比较。结果表明,BEI终浓度0.002mol/L、30℃24h是灭活水貂肠炎细小病毒的适宜参数,BEI灭活工艺制备的水貂肠炎细小病毒灭活疫苗具有良好的安全性,BEI灭活工艺与甲醛灭活工艺的免疫抗体水平差异不显著(P>0.05)。本研究为水貂肠炎细小病毒灭活工艺的研究提供了理论依据。
To observe the inactivation effect of binary ethylenimine(BEI) on mink enteritis virus(MEV),the mink enteritis virus was inactivated 24 h,48 h and 72 h after treatment with different concentration of BEI(0.003 mol/L,0.002 mol/L,0.001 mol/L,0.000 5 mol/L) at30℃.The assurance for BEI inactivation was performed by cytopathic effect(CPE) in F81 cell cultures and hemagglutination(HA) test.The minks were used to test the safety of the BEI-inactivated vaccine. The hemagglutination inhibition antibody from mink were used to evaluate the immune efficacy of the two kinds of vaccine,which were inactivated with BEI andformaldehyde. The results indicated that the suitable parameters of MEV inactivated by BEI is 0.002 mol/L of BEI,at 30℃,for 24 h. The vaccine inactivate with BEI was safe to the mink,and made a slightly higher HI antibody lever than the formaldehyde-inactivated vaccine. This study provides references for research of mink enteritis virus inactivated vaccine.
To observe the inactivation effect of binary ethylenimine(BEI) on mink enteritis virus(MEV),the mink enteritis virus was inactivated 24 h,48 h and 72 h after treatment with different concentration of BEI(0.003 mol/L,0.002 mol/L,0.001 mol/L,0.000 5 mol/L) at30℃.The assurance for BEI inactivation was performed by cytopathic effect(CPE) in F81 cell cultures and hemagglutination(HA) test.The minks were used to test the safety of the BEI-inactivated vaccine. The hemagglutination inhibition antibody from mink were used to evaluate the immune efficacy of the two kinds of vaccine,which were inactivated with BEI andformaldehyde. The results indicated that the suitable parameters of MEV inactivated by BEI is 0.002 mol/L of BEI,at 30℃,for 24 h. The vaccine inactivate with BEI was safe to the mink,and made a slightly higher HI antibody lever than the formaldehyde-inactivated vaccine. This study provides references for research of mink enteritis virus inactivated vaccine.
引文
[1]徐守振,尹燕博,王新.动物疫苗中常用抗原灭活剂的研究进展[J].中国畜牧兽医,2010,(9):162-167.
[2]傅元华,刘俊斌,钱钟,等.二乙烯亚胺对猪细小病毒的灭活作用[J].中国动物传染病学报,2012,20(1):73-76.
[3]王一鸣,应瑛,陈小庆,等.二乙烯亚胺和甲醛对猫杯状病毒灭活效果比较研究[J].中国兽药杂志,2015,49(5):15-19.
[4]张智明,王全杰,窦海艳,等[J].中国兽药杂志,2015,(6):9-12.
[5]Bahnemann H.G.Inactivation of viral antigens for vaccine preparation with particular reference to the application of binary ethylenimine[J].Vaccine,1990,8(4):299-303.
[2]傅元华,刘俊斌,钱钟,等.二乙烯亚胺对猪细小病毒的灭活作用[J].中国动物传染病学报,2012,20(1):73-76.
[3]王一鸣,应瑛,陈小庆,等.二乙烯亚胺和甲醛对猫杯状病毒灭活效果比较研究[J].中国兽药杂志,2015,49(5):15-19.
[4]张智明,王全杰,窦海艳,等[J].中国兽药杂志,2015,(6):9-12.
[5]Bahnemann H.G.Inactivation of viral antigens for vaccine preparation with particular reference to the application of binary ethylenimine[J].Vaccine,1990,8(4):299-303.