黄芪多糖注射液生物活性检测方法的分析与评价
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摘要
为了证实现有黄芪多糖生物活性检测方法的效果,并确定胸腺重和胸腺指数在黄芪多糖生物活性检测中的作用,使用《中国兽药典2015版(二部)》提供的检测方法,对河南省4个兽药厂提供的黄芪多糖样品进行了生物活性检测,测量了昆明鼠的体重、脾脏重和胸腺重等,计算了脾指数和胸腺指数。并对胸腺和脾脏两器官之间的关系进行了相关分析,对4种样品间的各测定项目作了比较。结果表明:4种样品中有2种合格2种不合格;黄芪多糖具有促进昆明鼠脾脏生长的作用,对昆明鼠胸腺的促进作用不明显,可能有一定抑制胸腺退化的作用;脾重和胸腺重之间、脾指数和胸腺指数之间均呈弱正相关关系;脾指数在反映黄芪多糖的生物活性方面是灵敏的,胸腺指数可以作为黄芪多糖生物活性检测的参考指标。
In order to confirm the effect of the existing Astragalus polysaccharide bioactivity detection method,and to determine the role of thymus weight and thymus index in the detection of Astragalus polysaccharide bioactivity,the detection method provided by Chinese Veterinary Pharmacopoeia 2015 Edition( Part 2) was used in the experiment. Biological activity of 4 Astragalus polysaccharides samples provided by the factoies in Henan were tested and the body weight,spleen weight and thymus weight of Kunming rats were measured,the spleen index and thymus index were calculated. Correlation analysis was performed on the relationship between the thymus and spleen organs,and the comparison between the four samples was made. The results showed that 2 samples were qualified and 2 were unqualified; Astragalus polysaccharide could promote the growth of spleen in Kunming mice,but it has no obvious effect on the thymus of Kunming rats and may has effect on inhibiting thymus degeneration;between spleen weight and thymus weight,the spleen index and thymus index were weakly positively correlated;the spleen index was sensitive in detecting the biological activity of Astragalus polysaccharides,and the thymus index could be used as a reference for the detection of Astragalus polysaccharide bioactivity.
In order to confirm the effect of the existing Astragalus polysaccharide bioactivity detection method,and to determine the role of thymus weight and thymus index in the detection of Astragalus polysaccharide bioactivity,the detection method provided by Chinese Veterinary Pharmacopoeia 2015 Edition( Part 2) was used in the experiment. Biological activity of 4 Astragalus polysaccharides samples provided by the factoies in Henan were tested and the body weight,spleen weight and thymus weight of Kunming rats were measured,the spleen index and thymus index were calculated. Correlation analysis was performed on the relationship between the thymus and spleen organs,and the comparison between the four samples was made. The results showed that 2 samples were qualified and 2 were unqualified; Astragalus polysaccharide could promote the growth of spleen in Kunming mice,but it has no obvious effect on the thymus of Kunming rats and may has effect on inhibiting thymus degeneration;between spleen weight and thymus weight,the spleen index and thymus index were weakly positively correlated;the spleen index was sensitive in detecting the biological activity of Astragalus polysaccharides,and the thymus index could be used as a reference for the detection of Astragalus polysaccharide bioactivity.
引文
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[3]万思敬.黄芪多糖稀释液对猪瘟疫苗免疫效果的影响[J].中国动物保健,2017,19(11):73-74.Wan S J. Effects of astragalus polysaccharide diluent on the immune efficacy of swine fever vaccine[J]. China Animal Health,2017,19(11):73-74.
[4]李朝辉,孙龙,王健,等.枯草芽孢杆菌和黄芪多糖对雏鸡免疫力的影响[J].吉林畜牧兽医,2019,40(1):5-8.Li Z H,Sun L,Wang J,et al. Effect of bacillus subtilis and astragalus polysaccharide on chicken immunity[J]. Jilin Animal Husbandry and Veterinary Medicine,2019,40(1):5-8.
[5]闫春贤.黄芪多糖对仔鸡机体免疫力的影响[J].时代报告,2012,(3):35.Yan C X. Effect of astragalus polysaccharide on body immunity of chicken[J]. Time Report,2012,(3):35.
[6]徐端红,贾新彦,胡梅.黄芪多糖对断奶羔羊生长性能及免疫力的影响[J].中国饲料,2018(12):56-59.Xu R H,Jia X Y,Hu M. Effects of astragalus polysaccharides on growth performance and immunity of weaning lambs[J]. China Feed,2018(12):56-59.
[7]宣小龙.黄芪多糖提高干奶期奶牛乳腺免疫力试验[J].草业与畜牧,2011(2):4-6.Xuan X L. Effect of astragalus polysaccharide on mammary gland immunity in dairy cows during dry period[J]. Prataculture&Animal Husbandry,2011(2):4-6.
[8]高扬,王洪芳,陈辉,等.饲粮添加黄芪多糖对蛋鸡免疫功能及肠道菌群的影响[J].动物营养学报,2011,23(3):447-451.Gao Y,Wang H F,Chen H,et al. Effects of dietary astragalus polysaccharides on immune function and intestinal flora in laying hens[J]. ACTA ZOONUTRIMENTA SINICA,2011,23(3):447-451.
[9]于小川,赵明晓,宋玉财,等.黄芪多糖在畜禽保健中的应用[J].山东畜牧兽医,2016,37(9):87-88.Yu X C,Zhao X M,Song Y C,et al. Application of astragalus polysaccharide in livestock and poultry health[J]. Shandong Journal of Animal Science and Veterinary Medicine,2016,37(9):87-88.
[10]侯晓礁,王海良,王秀敏,等.黄芪多糖粉对妊娠后期母猪生产性能的影响[J].饲料研究,2009(6):36-37.Hou X Q,Wang H L,Wang X M,et al. Effect of astragalus polysaccharide powder on production performance of sows in late pregnancy[J]. FEED RESEARCH,2009(6):36-37.
[11]许灵波.黄芪多糖在动物生产中应用的研究进展[J].中国畜牧杂志,2015,51(3):84-87.Xu L B. The research progress of astragalus polysaccharide application in animal production[J]. Chinese Journal of Animal Science,2015,51(3):84-87.