南阳黑猪与长白猪肉品质差异的机制研究
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摘要
选取180日龄南阳黑猪与长白猪各6头,总计12头,分别进行屠宰试验,用于测定2个品种猪背最长肌中脂肪酸合成酶(FAS)、激素敏感脂肪酶(HSL)、脂蛋白脂酶(LPL)和肉碱棕榈酰转移酶Ⅰ(CPT-Ⅰ)的活性差异,旨在阐明2个品种猪肉品质形成的差异机制。结果表明,与长白猪相比,南阳黑猪具有较低的HSL酶活性,而FAS、CPT-Ⅰ和LPL酶活性及FAS/HSL比值则显著性高于长白猪(P<0.05)。表明南阳黑猪优良肉品质的形成与肌肉组织中脂肪代谢相关酶活性间存在重要相关性。
引文
[1]Anderson F,Pannier L,Pethick D W,et al.Intramuscular fat in lamb muscle and the impact of selection for improved carcass lean meat yield[J].Animal,2015,9(6):1081-1090.
[2]Kouba M,Enser M,Whittington F M,et al.Effect of a high linolen in acid diet on lipogenic enzyme activities,fatty acid composition and meat quality in the growing pig[J].Journal of Animal Science,2003,81(8):1967-1979.
[3]Davoli R,Braglia S.Molecular approaches in pig breeding to improve meat quality[J].Briefings in Functional Genomic and Proteomics,2007,6(4):313-321.
[4]徐秋良,吴运香,张长兴,等.畜禽肉嫩度及其影响因素[J].家畜生态学报,2010,31(6):100-103.
[5]Fernandez X,Monin G,Talmant A,et al.Influence of intramuscular fat content on the quality of pig mea-1.Composition of the lipid fraction and sensory characteristics of m.longissimus lumborum[J].Meat Science,1999,53(1):59-65.
[6]刘作华,陈代文,龙定彪,等.日粮能量水平对猪脂肪酸合成酶和激素敏感酯酶的影响[J].中国畜牧杂志,2009,45(5):31-33,55.
[7]Etherton T D.The biology of somatotropin in adipose tissue growth and nutrient partitioning[J].Journal of Nutrition,2000,130(1):2623-2625.
[8]金宗濂,文镜,唐粉芬,等.功能食品评价原理及方法[M].北京:北京大学出版社,1995:86-95.
[9]黄其春.甜菜碱对肥育猪脂肪代谢及其关键酶基因表达的影响与机理研究[D].杭州:浙江大学,2006:81-85.
[10]Shillabeer G,Hornford J,Forden J M,et al.Hepatic and adipose tissue lipogenic enzyme mRNA level are suppressed by high fat diets in the rat[J].Journal of Lipid Research,1990,31(4):623-631.
[11]Bieber L L,Abraham T,Helmrath T.A rapid spectrophotometric assay for carnitine palmitoyltransferase[J].Analytical Biochemistry,1972,50(2):509-518.
[12]Dodson M V,Jiang Z H,Chen J,et al.Allied industry approaches to alter intramuscular fat content and composition in beef animals[J].Journal of Food Science,2010,75(1):R1-R8.
[13]Tong J,Zhu M J,Underwood K R,et al.AMP-activated protein kinase and adipogenesis in sheep fetal skeletal muscle and 3T3-L1 cells[J].Journal of Animal Science,2008,86(6):1296-1305.
[14]冯岗,孙宝忠,卢凌,等.肌内脂肪与背最长肌肉质、脂肪酸组成的关系[J].食品工业科技,2013,34(10):129-132.
[15]Mead J R,Irvine S A,Ramji D P.Lipoprotein lipase:structure,function,regulation,and role in disease[J].Journal of Molecular Medicine,2002,80(12):753-769.李丹丹,刘蛟,王英群,等.PTD-FNK蛋白对猪精子的冷冻保护作用及其机制[J].江苏农业科学,2019,47(11):217-221.
[2]Kouba M,Enser M,Whittington F M,et al.Effect of a high linolen in acid diet on lipogenic enzyme activities,fatty acid composition and meat quality in the growing pig[J].Journal of Animal Science,2003,81(8):1967-1979.
[3]Davoli R,Braglia S.Molecular approaches in pig breeding to improve meat quality[J].Briefings in Functional Genomic and Proteomics,2007,6(4):313-321.
[4]徐秋良,吴运香,张长兴,等.畜禽肉嫩度及其影响因素[J].家畜生态学报,2010,31(6):100-103.
[5]Fernandez X,Monin G,Talmant A,et al.Influence of intramuscular fat content on the quality of pig mea-1.Composition of the lipid fraction and sensory characteristics of m.longissimus lumborum[J].Meat Science,1999,53(1):59-65.
[6]刘作华,陈代文,龙定彪,等.日粮能量水平对猪脂肪酸合成酶和激素敏感酯酶的影响[J].中国畜牧杂志,2009,45(5):31-33,55.
[7]Etherton T D.The biology of somatotropin in adipose tissue growth and nutrient partitioning[J].Journal of Nutrition,2000,130(1):2623-2625.
[8]金宗濂,文镜,唐粉芬,等.功能食品评价原理及方法[M].北京:北京大学出版社,1995:86-95.
[9]黄其春.甜菜碱对肥育猪脂肪代谢及其关键酶基因表达的影响与机理研究[D].杭州:浙江大学,2006:81-85.
[10]Shillabeer G,Hornford J,Forden J M,et al.Hepatic and adipose tissue lipogenic enzyme mRNA level are suppressed by high fat diets in the rat[J].Journal of Lipid Research,1990,31(4):623-631.
[11]Bieber L L,Abraham T,Helmrath T.A rapid spectrophotometric assay for carnitine palmitoyltransferase[J].Analytical Biochemistry,1972,50(2):509-518.
[12]Dodson M V,Jiang Z H,Chen J,et al.Allied industry approaches to alter intramuscular fat content and composition in beef animals[J].Journal of Food Science,2010,75(1):R1-R8.
[13]Tong J,Zhu M J,Underwood K R,et al.AMP-activated protein kinase and adipogenesis in sheep fetal skeletal muscle and 3T3-L1 cells[J].Journal of Animal Science,2008,86(6):1296-1305.
[14]冯岗,孙宝忠,卢凌,等.肌内脂肪与背最长肌肉质、脂肪酸组成的关系[J].食品工业科技,2013,34(10):129-132.
[15]Mead J R,Irvine S A,Ramji D P.Lipoprotein lipase:structure,function,regulation,and role in disease[J].Journal of Molecular Medicine,2002,80(12):753-769.李丹丹,刘蛟,王英群,等.PTD-FNK蛋白对猪精子的冷冻保护作用及其机制[J].江苏农业科学,2019,47(11):217-221.