几种稻田固氮蓝藻挥发物的气相色谱-质谱分析
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摘要
[目的]分析稻田固氮蓝藻挥发性成分,评估其应用潜力。[方法]采用气相色谱-质谱联用(GC-MS)分析方法对5株稻田固氮蓝藻的挥发性化学成分进行分析与鉴定。[结果]固氮蓝藻挥发性成分中有多种生物活性功能的烷烃、酚类和酯类物质,具有抗动植物病原微生物、抑制有害藻类、抗草、杀虫等作用。[结论]稻田固氮蓝藻在生物源药物、杀藻剂和农药等生产领域具有应用价值。
[Objective] The aim was to analyze volatiles of several nitrogen-fixing cyanobacteria isolated from rice fields to evaluate its potential applications. [Method]The volatile compounds of five nitrogen-fixing cyanobacteria were isolated and identified by using gas chromatography-mass spectrometry. [Result]Some alkane,phenols and esters appeared in algae with some biological activities in inhibiting or killing animal and plant pathogenic microorganisms,harmful algae,weeds,insects,etc.. [Conclusion]It illustrates that nitrogen-fixing cyanobacteria isolated from rice-field can be used as biological source drug,pesticide and algicide.
[Objective] The aim was to analyze volatiles of several nitrogen-fixing cyanobacteria isolated from rice fields to evaluate its potential applications. [Method]The volatile compounds of five nitrogen-fixing cyanobacteria were isolated and identified by using gas chromatography-mass spectrometry. [Result]Some alkane,phenols and esters appeared in algae with some biological activities in inhibiting or killing animal and plant pathogenic microorganisms,harmful algae,weeds,insects,etc.. [Conclusion]It illustrates that nitrogen-fixing cyanobacteria isolated from rice-field can be used as biological source drug,pesticide and algicide.
引文
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[25]DONG S J,BI X D,WANG N,et al.Algicidal activities of Cladophora fracta on red tide-forming microalgae Heterosigma akashiwo and Gymnodinium breve[J].Allelopathy journal,2016,37(2):231-240.
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[27]BABU B,WU J T.Production of phthalate esters by nuisance freshwater algae and cyanobacteria[J].Science of the total environment,2010,408(21):
[2]SINGH N K,DHAR D W,TABASSUM R.Role of cyanobacteria in crop protection[J].Proceedings of the national academy of sciences,India section B:Biological sciences,2016,86(1):1-8.
[3]黎尚豪.固氮蓝藻作为晚稻肥源的研究[J].水生生物学集刊,1981,7(3):417-423.
[4]KHAIRY H M,EL-KASSAS H Y.Active substance from some blue green algal species used as antimicrobial agents[J].African journal of biotechnolog,2010,9(19):2789-2800.
[5]OZDEMIR G,KARABAY N U,DALAY M C,et al.Antibacterial activity of volatile component and various extracts of Spirulina platensis[J].Phytotherapy research,2004,18(9):754-757.
[6]殷献华,李天磊,潘卫东,等.葎草挥发油化学成分分析及其抑菌作用研究[J].山地农业生物学报,2010,29(5):415-418.
[7]ROELOFS W L,CARDR T.Hydrocarbon sex pheromone in tiger moths(Arctiidae)[J].Science,1971,171(3972):684-686.
[8]PADMAVATHI A R,BAKKIYARAJ D,THAJUDDIN N,et al.Effect of 2,4-di-tert-butylphenol on growth and biofilm formation by an opportunistic fungus Candida albicans[J].Biofouling,2015,31(7):565-574.
[9]VISZWAPRIYA D,PRITHIKA U,DEEBIKA S,et al.In vitro and in vivo antibiofilm potential of 2,4-Di-tert-butylphenolfrom seaweed surface associated bacterium Bacillus subtilis against group A streptococcus[J].Microbiological research,2016,191:19-31.
[10]PADMAVATHI A R,ABINAYA B,PANDIAN S K.Phenol,2,4-bis(1,1-dimethylethyl)of marine bacterial origin inhibits quorum sensing mediated biofilm formation in the uropathogen Serratia marcescens[J].Biofouling,2014,30(9):1111-1122.
[11]PADMAVATHI A R,PERIYASAMY M,PANDIAN S K.Assessment of 2,4-Di-tert-butylphenol induced modifications in extracellular polymeric substances of Serratia marcescens[J].Bioresource technology,2015,188:185-189.
[12]CHUAH T S,NORHAFIZAH M Z,NAIMAH A H,et al.Phytotoxic activity of the allelochemical,2,4-di-tert-butylphenol on two selected weed species[J].Sains malaysiana,2016,45(6):963-967.
[13]DHARNI S,SANCHITA,MAURYA A,et al.Purification,characterization,and in vitro activity of 2,4-di-tertbutylphenol from Pseudomonas monteilii Ps F84:Conformational and molecular docking studies[J].Journal of agricultural and food chemistry,2014,62(26):6138-6146.
[14]CHOI S J,KIM J K,KIM H K,et al.2,4-di-tert-butylphenol from sweet potato protects against oxidative stress in Pc12 cells and in mice[J].Journal of medicinal food,2013,16(11):977-983.
[15]王再强,吴毅歆,周红,等.2,4-二叔丁基苯酚对秀丽隐杆线虫的毒杀作用[J].农药,2014,53(4):298-300.
[16]周宝利,李娜,刘双双,等.2,4-二叔丁基苯酚对番茄叶霉病及幼苗生长的影响[J].生态学杂志,2013,32(5):1203-1207.
[17]GAO Z F,ZHANG B J,LIU H P,et al.Identification of endophytic Bacillus velezensis ZSY-1 strain and antifungal activity of its volatile compounds against Alternaria solani and Botrytis cinerea[J].Biological control,2017,105:27-39.
[18]LIU N,WEN F L,LI F M,et al.Inhibitory mechanism of phthalate esters on Karenia brevis[J].Chemosphere,2016,155:498-508.
[19]张胜娟,夏文彤,杨晓辉,等.槐叶萍抑藻效应及其抑藻物质的分离鉴定[J].卫生研究,2016,45(3):442-447.
[20]ZHANG P,ZHAI C M,WANG X X,et al.Growth competition between Microcystis aeruginosa and Quadrigula chodatii under controlled conditions[J].Journal of applied phycology,2013,25(2):555-565.
[21]CHOE S,JUNG I H.Growth inhibition of freshwater algae by ester compounds released from rotted plants[J].Journal of industrial and engineering chemistry,2002,8(4):297-304.
[22]周宝利,陈丰,刘娜,等.邻苯二甲酸二异丁酯对茄子黄萎病及其幼苗生长的化感作用[J].西北农业学报,2010,19(4):179-183.
[23]AHSAN T,CHEN J G,ZHAO X X,et al.Extraction and identification of bioactive compounds(eicosane and dibutyl phthalate)produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani[J].Amb express,2017,7:54.
[24]白雪娜,谷继成,成军,等.瑞香狼毒根的超临界萃取物抑菌活性和成分分析[J].林业科学,2012,48(2):169-174.
[25]DONG S J,BI X D,WANG N,et al.Algicidal activities of Cladophora fracta on red tide-forming microalgae Heterosigma akashiwo and Gymnodinium breve[J].Allelopathy journal,2016,37(2):231-240.
[26]贾双双.番茄砧木对南方根结线虫抗性鉴定及抗性机制研究[D].泰安:山东农业大学,2012.
[27]BABU B,WU J T.Production of phthalate esters by nuisance freshwater algae and cyanobacteria[J].Science of the total environment,2010,408(21):