cIAP与Caspase-7在子宫内膜腺癌组织中的表达及其与预后的关系研究
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摘要
目的探索细胞凋亡抑制蛋白cIAP和凋亡蛋白Caspase-7在子宫内膜癌组织中的表达及与预后的关系。方法选取2009~2010年在河南科技大学第一附属医院行子宫内膜癌根治且术后病理证实为子宫内膜腺癌患者55例,统计发病时组织学分类、病理分期、淋巴结转移、年龄等参数,电话随访无瘤生存期及总生存期,取病理标本石蜡切片行免疫组化,观察cIAP与Caspase-7的表达,并分析其与各参数的关系。结果 cIAP与Caspase-7的表达水平与子宫内膜癌组织学分类、病理分期、淋巴结转移相关(P<0.05),而与肌层浸润、年龄无关(P>0.05)。cIAP与Caspase-7阳性表达和阴性表达的患者总生存期之间差异有统计学意义(P<0.05)。结论子宫内膜腺癌患者cIAP与Caspase-7的表达水平与肿瘤分级、总生存期具有相关性,或可作为子宫内膜腺癌预后检测指标。
Objective To study the expression of cIAP and Caspase-7in endometrial adenocarcinoma tissue and its relation with prognosis.Methods Fifty-five cases tissues of endometrial adenocarcinoma patients were detected by immunohistochemistry assay from the First Affiliated Hospital of Henan University of Science and Technology from 2009 to 2010.Histological classification,pathological stage,lymph node metastasis,age and other parameters were collected,the survival and overall survival of the tumor were followed up,immunohistochemistry was used to observe the expression of cIAP and Caspase-7in paraffin sections of pathological specimens,and the relationship between them and the parameters were analyzed.Results The expression level of cIAP and Caspase-7was related to the histological classification,pathological stage and lymph node metastasis of endometrial carcinoma(P<0.05),but it was not related with the age,muscular layer infiltration(P>0.05).There was significant difference between the positive expression and the negative expression of cIAP and Caspase-7(P<0.05).Conclusion The expression level of cIAP and Caspase-7in patients with endometrial carcinoma is correlated with tumor grade and overall survival,or can be used as a prognostic indicator for endometrial cancer.
Objective To study the expression of cIAP and Caspase-7in endometrial adenocarcinoma tissue and its relation with prognosis.Methods Fifty-five cases tissues of endometrial adenocarcinoma patients were detected by immunohistochemistry assay from the First Affiliated Hospital of Henan University of Science and Technology from 2009 to 2010.Histological classification,pathological stage,lymph node metastasis,age and other parameters were collected,the survival and overall survival of the tumor were followed up,immunohistochemistry was used to observe the expression of cIAP and Caspase-7in paraffin sections of pathological specimens,and the relationship between them and the parameters were analyzed.Results The expression level of cIAP and Caspase-7was related to the histological classification,pathological stage and lymph node metastasis of endometrial carcinoma(P<0.05),but it was not related with the age,muscular layer infiltration(P>0.05).There was significant difference between the positive expression and the negative expression of cIAP and Caspase-7(P<0.05).Conclusion The expression level of cIAP and Caspase-7in patients with endometrial carcinoma is correlated with tumor grade and overall survival,or can be used as a prognostic indicator for endometrial cancer.
引文
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[2]Lopez J,Wicky Joh S,Tenev T,et al.CARD mediated autoinhibition of cIAP1′s E3ligase activity suppresses cell proliferation and migration[J].Moleculear Cell 2011,42(5):569-583.
[3]Li X,Wu K,Fan D.CIAPIN1as a therapeutic target in cancer[J].Expert Opin Ther Targets,2010,14(6):603-610.
[4]郄明蓉,张竹.子宫内膜癌流行病学及发病因素[J].中国实用妇科及产科杂志,2011,27(11):34-37.
[5]Creasman WT,Odicino F,Maisonneuve P,et al.Carcinoma of the corpus uteri FIGO 6th Annual Report on the Results of Treatment in Gynecological Cancer[J].Int J Gynaecol Obstet,2006,95(Suppl 1):S105-143.
[6]Begg AC,Stewart FA,Vens C.Strategies to improve radiotherapy with targeted drugs[J].Nat Rev Cancer,2011,137(11):239-253.
[7]Park JS,Shin DY,Lee YW,et al.Apoptotic and anti-metastatic effects of the whole skin of Venenum bufonis in A549human lung cancer cells[J].Int J Oncol,2012,40(4):1210-1219.
[8]Psyrri A,Yu Z,Bamias A,et al.Evaluation of the prognostic value of cellular inhibitor of apoptosis protein in epithelial ovarian cancer using automated quantitative protein analysis[J].Cancer Epidemiol Biomarkers Prev Journal Article,2006,15(6):1179-1183.
[9]Gordon GJ,Mani M,Mukhopadhyay L,et al.Inhibitor of apoptosis proteins are regulated by tumour necrosis factor-a malignant pleural[J].Mesothelioma Pathol,2007,21(4):439-446.
[10]Montagut C,Rovira A,Albanell J.The proteasome:a novel target for anticancer therapy[J].Clin Transl Oncol,2006,8(5):313-317.