Ck2α、β-catenin、c-IAP1及survivin联合检测在宫颈癌诊断中的临床意义
|
摘要
目的研究酪蛋白激酶2α(Ck2α)、β-连环蛋白(β-catenin)、凋亡抑制蛋白(c-IAP1)及存活素(survivin)在宫颈癌组织中的表达情况及其相关性,探讨四者联合检测在宫颈癌诊断中的临床意义。方法选择重庆市大足区人民医院收治的宫颈癌74例(宫颈癌组)、宫颈上皮内瘤变(CIN)83例(CIN组)和子宫肌瘤50例(对照组),采用免疫组化法检测3组宫颈组织中Ck2α、β-catenin、c-IAP1及survivin表达情况,分析其相关性及与临床病理特征的关系。结果宫颈癌组Ck2α、β-catenin、c-IAP1、survivin阳性表达率高于CIN组和对照组,且其表达与肿瘤分化程度及FIGO分期有关(P<0.05),Ck2α阳性表达率与β-catenin、c-IAP1、survivin表达水平均呈正相关(r=0.570、0.391、0.476,P<0.05),Ck2α、β-catenin、c-IAP1、survivin阳性表达率与分化程度呈负相关(r=-0.325、-0.633、-0.397、-0.324,P<0.05),与FIGO分期呈正相关(r=0.349、0.634、0.621、0.391,P<0.05)。结论 Ck2α、β-catenin、c-IAP1及survivin在宫颈癌组织中阳性表达率较高,其与宫颈癌的发生、发展有关,四者联合检测对早期诊断宫颈癌及临床治疗有重要意义。
Objective To study the expressions and correlations of casein kinase2α(Ck2α),β-catenin,c-inhibitor of apoptosis protein1( c-IAP1) and survivin in cervical cancer tissues and to analyze the clinical value of combined detection in diagnosis of cervical cancer. Methods A total of 74 patients with cervical cancer( CC group),83 patients with cervical intraepithelial neoplasia( CIN group) and 50 women with vterine fibroids( control group) were recruited in this study. Immunohistochemistry staining was used to detect the Ck2α,β-catenin,c-IAP1 and survivin expressions in the three groups,and the correlations and clinicopathological characteristics were also analyzed. Results The positive expression rates of Ck2α,β-catenin,c-IAP1 and survivin in CC group were significantly higher than those in CIN and control groups,and the expression levels were related with differential degrees of neoplasm and FIGO stages(P < 0. 05). In CC group,the positive expression rate of Ck2α was positively correlated with the expression levels of β-catenin,c-IAP1 and survivin( r = 0. 570,0. 391,0. 476,P < 0. 05),and the positive expression rates of Ck2α,β-catenin,c-IAP1 and survivin were negatively correlated with differential degrees( r =- 0. 325,- 0. 633,- 0. 397,-0. 324,P < 0. 05),but the rates were positively correlated with FIGO stages( r = 0. 349,0. 634,0. 621,0. 391,P <0. 05). Conclusion The Ck2α,β-catenin,c-IAP1 and survivin have high positive expression rates in cervical cancer tissues,and the rate are correlated with the occurrence and development of cervical cancer,therefore the combined detection has important clinical value in early diagnosis of cervical cancer.
Objective To study the expressions and correlations of casein kinase2α(Ck2α),β-catenin,c-inhibitor of apoptosis protein1( c-IAP1) and survivin in cervical cancer tissues and to analyze the clinical value of combined detection in diagnosis of cervical cancer. Methods A total of 74 patients with cervical cancer( CC group),83 patients with cervical intraepithelial neoplasia( CIN group) and 50 women with vterine fibroids( control group) were recruited in this study. Immunohistochemistry staining was used to detect the Ck2α,β-catenin,c-IAP1 and survivin expressions in the three groups,and the correlations and clinicopathological characteristics were also analyzed. Results The positive expression rates of Ck2α,β-catenin,c-IAP1 and survivin in CC group were significantly higher than those in CIN and control groups,and the expression levels were related with differential degrees of neoplasm and FIGO stages(P < 0. 05). In CC group,the positive expression rate of Ck2α was positively correlated with the expression levels of β-catenin,c-IAP1 and survivin( r = 0. 570,0. 391,0. 476,P < 0. 05),and the positive expression rates of Ck2α,β-catenin,c-IAP1 and survivin were negatively correlated with differential degrees( r =- 0. 325,- 0. 633,- 0. 397,-0. 324,P < 0. 05),but the rates were positively correlated with FIGO stages( r = 0. 349,0. 634,0. 621,0. 391,P <0. 05). Conclusion The Ck2α,β-catenin,c-IAP1 and survivin have high positive expression rates in cervical cancer tissues,and the rate are correlated with the occurrence and development of cervical cancer,therefore the combined detection has important clinical value in early diagnosis of cervical cancer.
引文
[1]潘惠艳,赵群,詹阳,等.RNAi干扰电压门控钠通道scn8a基因对人宫颈癌Si Ha细胞侵袭转移的影响[J].首都医科大学学报,2012,33(3):350-355.
[2]高琴,张丹丹,白帆,等.紫杉醇联合铂类方案治疗中晚期宫颈癌患者的临床疗效及对血清CYFRA21-1、SCC-Ag的影响[J].疑难病杂志,2015,14(1):53-56.
[3]段敬贤.HPV-DNA与血清SCCA检测在宫颈癌筛查中的意义[J].解放军预防医学杂志,2014,32(8):344-345.
[4]张海萌,李丽,隋霜,等.不同生殖道部位人乳头瘤病毒采样对宫颈癌筛查效果的比较[J].中国医药,2015,10(1):37-41.
[5]朱明玥,陈帆,高冬梅,等.c-IAP1在宫颈癌中的表达及临床意义[J].中国妇幼保健,2014,29(14):2253-2257.
[6]Filhol O,Cochet C.Protein kinase CK2 and cancer:further clues are accumulating[J].Bull Cancer,2002,89(3):261-265.
[7]Morin P J.beta-catenin signaling and cancer[J].Bioessays,1999,21(12):1021-1030.
[8]周琦,叶学正,唐郢,等.survivin与宫颈癌前病变及宫颈癌关系的研究[J].重庆医学,2006,35(5):427-429.
[9]Lee J P,Chang K H,Han J H,et al.Survivin,a novel anti-apoptosis inhibitor,expression in uterine cervical cancer and relationship with prognostic factors[J].Int J Gynecol Cancer,2005,15(1):113-119.
[10]Pecorelli S.Revised FIGO staging for carcinoma of the vulva,cervix,and endometrium[J].Int J Gynaecol Obstet,2009,105(2):103-104.
[11]吴爽,刘乙蒙,刘超,等.Ck2α、β-catenin和survivin在卵巢癌组织中表达的检测及其临床应用价值[J].吉林大学学报:医学版,2014(6):1256-1260.
[12]黄轶轩,周少华,薛洪省,等.蛋白激酶CK2与人类恶性肿瘤[J].中国肺癌杂志,2012,15(7):439-445.
[13]Faust R A,Niehans G,Gapany M,et al.Subcellular immunolocalization of protein kinase CK2 in normal and carcinoma cells[J].Int J Biochem Cell Biol,1999,31(9):941-949.
[14]Trembley J H,Wang G,Unger G,et al.Protein kinase CK2 in health and disease:CK2:a key player in cancer biology[J].Cell Mol Life Sci,2009,66(11-12):1858-1867.
[15]Chakraborty A,Werner J K,Koldobskiy M A,et al.Casein kinase-2 mediates cell survival through phosphorylation and degradation of inositol hexakisphosphate kinase-2[J].Proc Natl Acad Sci U S A,2011,108(6):2205-2209.
[16]史慧星,陈云,游庆朋,等.β-连环蛋白与细胞周期蛋白D1在宫颈鳞癌中的表达及意义[J].中国全科医学,2010,13(32):3642-3645.
[17]郑洪,杨光华,张剑,等.c-IAP1和c-IAP2及Survivin在肝细胞性肝癌中的表达[J].贵阳医学院学报,2003,28(3):192-196.
[18]尚培中,王金,马学柱,等.生存素在胰腺癌组织中的表达及其临床意义[J].解放军医药杂志,2013,25(3):47-49.
[19]郑力强,韩向春,张凯,等.阴茎鳞癌中survivin和caspase-3蛋白的表达及其与腹股沟淋巴结转移的关系[J].西北国防医学杂志,2010,31(2):114-116.
[20]朱利利,熊正文,李永申,等.Wnt信号转导通路与女性常见肿瘤的关系[J].解放军医药杂志,2013,25(6):94-96.
[2]高琴,张丹丹,白帆,等.紫杉醇联合铂类方案治疗中晚期宫颈癌患者的临床疗效及对血清CYFRA21-1、SCC-Ag的影响[J].疑难病杂志,2015,14(1):53-56.
[3]段敬贤.HPV-DNA与血清SCCA检测在宫颈癌筛查中的意义[J].解放军预防医学杂志,2014,32(8):344-345.
[4]张海萌,李丽,隋霜,等.不同生殖道部位人乳头瘤病毒采样对宫颈癌筛查效果的比较[J].中国医药,2015,10(1):37-41.
[5]朱明玥,陈帆,高冬梅,等.c-IAP1在宫颈癌中的表达及临床意义[J].中国妇幼保健,2014,29(14):2253-2257.
[6]Filhol O,Cochet C.Protein kinase CK2 and cancer:further clues are accumulating[J].Bull Cancer,2002,89(3):261-265.
[7]Morin P J.beta-catenin signaling and cancer[J].Bioessays,1999,21(12):1021-1030.
[8]周琦,叶学正,唐郢,等.survivin与宫颈癌前病变及宫颈癌关系的研究[J].重庆医学,2006,35(5):427-429.
[9]Lee J P,Chang K H,Han J H,et al.Survivin,a novel anti-apoptosis inhibitor,expression in uterine cervical cancer and relationship with prognostic factors[J].Int J Gynecol Cancer,2005,15(1):113-119.
[10]Pecorelli S.Revised FIGO staging for carcinoma of the vulva,cervix,and endometrium[J].Int J Gynaecol Obstet,2009,105(2):103-104.
[11]吴爽,刘乙蒙,刘超,等.Ck2α、β-catenin和survivin在卵巢癌组织中表达的检测及其临床应用价值[J].吉林大学学报:医学版,2014(6):1256-1260.
[12]黄轶轩,周少华,薛洪省,等.蛋白激酶CK2与人类恶性肿瘤[J].中国肺癌杂志,2012,15(7):439-445.
[13]Faust R A,Niehans G,Gapany M,et al.Subcellular immunolocalization of protein kinase CK2 in normal and carcinoma cells[J].Int J Biochem Cell Biol,1999,31(9):941-949.
[14]Trembley J H,Wang G,Unger G,et al.Protein kinase CK2 in health and disease:CK2:a key player in cancer biology[J].Cell Mol Life Sci,2009,66(11-12):1858-1867.
[15]Chakraborty A,Werner J K,Koldobskiy M A,et al.Casein kinase-2 mediates cell survival through phosphorylation and degradation of inositol hexakisphosphate kinase-2[J].Proc Natl Acad Sci U S A,2011,108(6):2205-2209.
[16]史慧星,陈云,游庆朋,等.β-连环蛋白与细胞周期蛋白D1在宫颈鳞癌中的表达及意义[J].中国全科医学,2010,13(32):3642-3645.
[17]郑洪,杨光华,张剑,等.c-IAP1和c-IAP2及Survivin在肝细胞性肝癌中的表达[J].贵阳医学院学报,2003,28(3):192-196.
[18]尚培中,王金,马学柱,等.生存素在胰腺癌组织中的表达及其临床意义[J].解放军医药杂志,2013,25(3):47-49.
[19]郑力强,韩向春,张凯,等.阴茎鳞癌中survivin和caspase-3蛋白的表达及其与腹股沟淋巴结转移的关系[J].西北国防医学杂志,2010,31(2):114-116.
[20]朱利利,熊正文,李永申,等.Wnt信号转导通路与女性常见肿瘤的关系[J].解放军医药杂志,2013,25(6):94-96.