超灵敏NO_2~-纳米传感器的研究及褐藻中NO_2~-的检测
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摘要
现阶段,低值食品原料(如褐藻)引起人们的重视,低值资源的利用也列入了国家十三五计划,但是,由于环境污染,褐藻中的亚硝酸盐是食品安全的重大威胁。本研究利用纳米技术与传统检测手段相结合的方法,大大降低了检测限,改进了常用的亚硝酸盐的检测方法(比色法),也就是对氨基苯硫酚和萘基乙二胺分别修饰到金纳米颗粒上得到两种功能化金纳米颗粒,进行重氮化耦合反应后,金纳米颗粒的光学性能发生变化,这种变化与体系中的亚硝酸盐的含量呈正相关。金纳米颗粒对信号响应起到了放大的效果。改进后的方法的检测限达到了4 ng/mL,而且操作简单,成本低,稳定性好,适用于记录低值食品原料中微量亚硝酸盐的产生过程。本研究是新兴的纳米技术与传统检测技术的结合,纳米技术促进了食品检测行业的发展,也是食品工业发展必不可少的关键一步。
Nowadays,low-value food raw materials(such as brown algae)have attracted people's attention. the use of low-value resources were included in The National 13 th Five-Year Plan. However,due to environmental pollution,nitrite in brown algae is a major threat to food safety. In this study,the combination of nanotechnology and traditional detection methods were used to greatly reduce the detection limit and improved the commonly nitrite detection method(colorimetric method). The p-aminothiophenol and naphthylethylenediamine were respectively modified onto gold nanoparticles to obtain two functionalized gold nanoparticles. After the diazotization coupling reaction,the optical properties of the gold nanoparticles have changed which was positively correlated with content of nitrite. Gold nanoparticles act to amplify the signal response. The improved method had a detection limit of4 ng/mL,and was simple in operation,low in cost and good in stability,which was suitable for recording the production process of trace nitrite in low-value food materials. This study was a combination of emerging nanotechnology and traditional detection technology which had promoted the development of the food testing industry and was an essential step in the development of the food industry.
Nowadays,low-value food raw materials(such as brown algae)have attracted people's attention. the use of low-value resources were included in The National 13 th Five-Year Plan. However,due to environmental pollution,nitrite in brown algae is a major threat to food safety. In this study,the combination of nanotechnology and traditional detection methods were used to greatly reduce the detection limit and improved the commonly nitrite detection method(colorimetric method). The p-aminothiophenol and naphthylethylenediamine were respectively modified onto gold nanoparticles to obtain two functionalized gold nanoparticles. After the diazotization coupling reaction,the optical properties of the gold nanoparticles have changed which was positively correlated with content of nitrite. Gold nanoparticles act to amplify the signal response. The improved method had a detection limit of4 ng/mL,and was simple in operation,low in cost and good in stability,which was suitable for recording the production process of trace nitrite in low-value food materials. This study was a combination of emerging nanotechnology and traditional detection technology which had promoted the development of the food testing industry and was an essential step in the development of the food industry.
引文
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[14]J Yang,D Shen,L Zhou,et al.Mesoporous silica-coated plasmonic nanostructures for surface-enhanced Raman scattering detection and photothermal therapy[J].Advanced《中国食品添加剂》杂欢迎您通过唯一渠道-本刊在网址:http:/Healthcare Materials,2014(3):1620-1628.
[15]P Gobbo,M C Biesinger,M S Workentin.Facile synthesis of gold nanoparticle(AuNP)-carbon nanotube(CNT)hybrids through an interfacial Michael addition reaction[J].Chemical Communications,2013(49)2831-2840.
[16]H X Su,Z H Zhang,X Y Zhao,et al.The LambertBeer’s law characterization of formal analysis in Terahertz spectrum quantitative testing[J].Spectroscopy&Spectral Analysis,2013(33):3180-3192.
[17]Y Liu,R Zhong,P Zhang,er al.Understanding the robust physisorption between bovine serum albumin and amphiphilic polymer coated nanoparticles[J].Acs.Applied Materials&Interfaces,2015(8):67-72.
[18]D Tsikas,A Schwarz,D O Stichtenoth.Simultaneous measurement of[15N]nitrate and[15N]nitrite enrichment and concentration in urine by gas chromatography mass spectrometry as pentafluorobenzyl derivatives[J].Analytical Chemistry,2017(82):2585-2587.
[19]D Tsikas,A B?hmer,A Mitschke.Gas chromatographymass spectrometry analysis of nitrite in biological fluids without derivatization[J].Analytical Chemistry,2010(82):5384-5390.
[20]D Tsikas,A Mitschke,F M Gutzki,et al.Evidence by gas chromatography-mass spectrometry of ex vivo nitrite and nitrate formation from air nitrogen oxides in human plasma,serum,and urine samples[J].Analytical Biochemistry,2010(397):126-128.
[21]K Ito,R Nomura,T Fujii,et al.Determination of n i t r i t e,n i t r a t e,b r o m i d e,a n d i o d i d e i n s e a w a t e r b y i o n c h r o m a t o g r a p h y w i t h U V d e t e c t i o n u s i n g dilauryldimethylammonium-coated monolithic ODS columns and sodium chloride as an eluent[J].Analytical&Bioanalytical Chemistry,2012(404):2513-2528.志-中国科技核心期刊中国知网的期刊编审系统投稿stj.cbpt.cnki.net/
[2]宋瑱,范高超,战书函,等.金纳米颗粒掺杂PEDOT多孔导电聚合物的电化学合成及其亚硝酸盐传感应用[J].分析测试学报,2018,37(10):1251-1257.
[3]Z Fredj,S Azzouzi,A P F Turner,et al.Neutravidin biosensor for direct capture of dual-functional biotin-molecular beacon-AuNP probe for sensitive voltammetric detection of microRNA[J].Sensors&Actuators B Chemical,2017(248):77-84.
[4]李贝贝,李屹,刘惠亮.金纳米颗粒在生物医学领域中的应用[J].中华灾害救援医学,2018,6(10):595-598.
[5]R Zhong,Y Liu,P Zhang,J Liu,et al.Discrete nanoparticle-BSA conjugates manipulated by hydrophobic interaction[J].Acs.Appl.Mater Interfaces,2014(6)19465-19470.
[6]D Tsikas,M T Suchy,A Mitschke,et al.Measurement of nitrite in urine by gas chromatography-mass spectrometry[J].Humana Press,2012(4):73-81.
[7]D Yu,D Yong,S Dong.Toxicity detection of sodium nitrite,borax and aluminum potassium sulfate using electrochemical method[J].环境科学学报(英文版),2013(25)785-790.
[8]Z Feng.Determination of nitrite in pickled food by electrochemical method[J].Journal of Chinese Institute of Food Science&Technology,2011(11):192-196.
[9]S Radhakrishnan,K Krishnamoorthy,C Sekar,et al.Ahighly sensitive electrochemical sensor for nitrite detection based on Fe2O3 nanoparticles decorated reduced graphene oxide nanosheets[J].Applied Catalysis B Environmental,2014(148-149):22-28.
[10]P Miao,M Shen,L Ning,et al.,Functionalization of platinum nanoparticles for electrochemical detection of nitrite[J].Analytical&Bioanalytical Chemistry,2017(399):2407-2421.
[11]Zhao,Lianchaoa,B.Yan,Hongtao.A nitrite microfluidic detecting chip by fabricating chlorinated polyvinyl chloride barrier in filter paper[J].Acta Chimica Sinica,2012(70)1104-1113.
[12]C Zhang,X Li,C Tian,G Yu,et al.DNA nanocages swallow gold nanoparticles(AuNPs)to form AuNP@DNAcage core-shell structures[J].Acs.Nano.,2014(8):1130-1135.
[13]M Stobiecka.Novel plasmonic field-enhanced nanoassay for trace detection of proteins[J].Biosensors&Bioelectronics,2014(55)379-385.
[14]J Yang,D Shen,L Zhou,et al.Mesoporous silica-coated plasmonic nanostructures for surface-enhanced Raman scattering detection and photothermal therapy[J].Advanced《中国食品添加剂》杂欢迎您通过唯一渠道-本刊在网址:http:/Healthcare Materials,2014(3):1620-1628.
[15]P Gobbo,M C Biesinger,M S Workentin.Facile synthesis of gold nanoparticle(AuNP)-carbon nanotube(CNT)hybrids through an interfacial Michael addition reaction[J].Chemical Communications,2013(49)2831-2840.
[16]H X Su,Z H Zhang,X Y Zhao,et al.The LambertBeer’s law characterization of formal analysis in Terahertz spectrum quantitative testing[J].Spectroscopy&Spectral Analysis,2013(33):3180-3192.
[17]Y Liu,R Zhong,P Zhang,er al.Understanding the robust physisorption between bovine serum albumin and amphiphilic polymer coated nanoparticles[J].Acs.Applied Materials&Interfaces,2015(8):67-72.
[18]D Tsikas,A Schwarz,D O Stichtenoth.Simultaneous measurement of[15N]nitrate and[15N]nitrite enrichment and concentration in urine by gas chromatography mass spectrometry as pentafluorobenzyl derivatives[J].Analytical Chemistry,2017(82):2585-2587.
[19]D Tsikas,A B?hmer,A Mitschke.Gas chromatographymass spectrometry analysis of nitrite in biological fluids without derivatization[J].Analytical Chemistry,2010(82):5384-5390.
[20]D Tsikas,A Mitschke,F M Gutzki,et al.Evidence by gas chromatography-mass spectrometry of ex vivo nitrite and nitrate formation from air nitrogen oxides in human plasma,serum,and urine samples[J].Analytical Biochemistry,2010(397):126-128.
[21]K Ito,R Nomura,T Fujii,et al.Determination of n i t r i t e,n i t r a t e,b r o m i d e,a n d i o d i d e i n s e a w a t e r b y i o n c h r o m a t o g r a p h y w i t h U V d e t e c t i o n u s i n g dilauryldimethylammonium-coated monolithic ODS columns and sodium chloride as an eluent[J].Analytical&Bioanalytical Chemistry,2012(404):2513-2528.志-中国科技核心期刊中国知网的期刊编审系统投稿stj.cbpt.cnki.net/