豨莶草提取物对小鼠的镇静催眠作用
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摘要
【目的】研究豨莶草提取物对小鼠的镇静催眠作用。【方法】将50只小鼠随机均分为空白组、艾司唑仑组(0.4 mg/kg)和豨莶草提取物低、中、高剂量组(400、800、1 600 mg/kg)。通过旷场实验、阈上剂量和阈下剂量戊巴比妥钠诱导小鼠睡眠实验,分别观察豨莶草提取物对小鼠自主活动、入睡潜伏期、睡眠持续时间和入睡比例的影响。【结果】与空白组相比,豨莶草提取物(800、1 600 mg/kg)能明显减少小鼠自主活动次数(P<0.01),延长睡眠持续时间(P<0.05),且提高注射了阈下剂量戊巴比妥钠小鼠的入睡比例(P<0.05,P<0.01)。【结论】豨莶草提取物对小鼠具有显著的镇静催眠作用,具有开发应用的价值。
【Objective】To study the sedative and hypnotic effects of Siegesbeckia herba extract in mice.【Methods】Fifty mice were randomly divided into control group, estazolam group(0.4 mg/kg) and Siegesbeckia herba extract groups in low, middle and high doses(400, 800, 1 600 mg/kg). The locomotor activity, latency to persistent sleep, sleep duration and sleep rate were determined respectively in mice via the open field test and injection of pentobarbital sodium in subthreshold and suprathreshold doses.【Results】Compared with the control group, Siegesbeckia herba extract(800, 1 600 mg/kg) significantly decreased the locomotor activity of mice(P<0.01),extended the sleep duration(P<0.05) and increased sleep rate of mice in subthreshold and suprathreshold doses of pentobarbital sodium, respectively(P<0.05, P<0.01).【Conclusion】Siegesbeckia herba extract has significant sedative and hypnotic effects on mice, and it is of application value.
【Objective】To study the sedative and hypnotic effects of Siegesbeckia herba extract in mice.【Methods】Fifty mice were randomly divided into control group, estazolam group(0.4 mg/kg) and Siegesbeckia herba extract groups in low, middle and high doses(400, 800, 1 600 mg/kg). The locomotor activity, latency to persistent sleep, sleep duration and sleep rate were determined respectively in mice via the open field test and injection of pentobarbital sodium in subthreshold and suprathreshold doses.【Results】Compared with the control group, Siegesbeckia herba extract(800, 1 600 mg/kg) significantly decreased the locomotor activity of mice(P<0.01),extended the sleep duration(P<0.05) and increased sleep rate of mice in subthreshold and suprathreshold doses of pentobarbital sodium, respectively(P<0.05, P<0.01).【Conclusion】Siegesbeckia herba extract has significant sedative and hypnotic effects on mice, and it is of application value.
引文
[1]中华医学会神经病学分会睡眠障碍学组.中国成人失眠诊断与治疗指南[J].中华神经科杂志,2012,45(7):534-540.
[2]张丽萍,夏猛.失眠症的治疗现状分析及思考[J].环球中医药,2011,4(1):66-69.
[3]赵忠新,张照环.应给予睡眠更多的关注[J].中华神经科杂志,2011,44(8):513-515.
[4]Mallory LJ,Taylor DJ,Lichstein KL,et al.Epidemiology of insomnia and medical disorders[J].SLEEP,2006,29:245-246.
[5]范英兰,张宏,甘雨,等.抗失眠中药复方对KM小鼠戊巴比妥钠催眠作用影响随机平行对照研究[J].实用中医内科杂志,2016,30(9):90-94.
[6]国家药典委员会.中华人民共和国药典-四部:2015年版分辑号:四部[M].北京:中国医药科技出版社,2015.368.
[7]罗琼,汪建平,阮金兰,等.豨莶草局部外用的抗炎镇痛作用研究[J].湖北中医学院学报,2008,10(3):9-11.
[8]胡慧华,汤鲁霞,李小猛.豨莶草生品和炮制品抗炎、抗风湿作用的实验研究[J].中国中药杂志,2004,29(6):542-545.
[9]钱瑞琴,张春英,付宏征.豨莶草活性部位抗风湿作用机理研究[J].中国中西医结合杂志,2000,20(3):192-195.
[10]Kim JY,Lim HJ,Ryu JH.In vitro anti-inflammatory activity of 3-O-methyl-flavones isolated from Siegesbeckia glabrescens[J].Bioorg Med Chem Lett,2008,18(4):1511-1514.
[11]Park HJ,Kim IT,Won JH,et al.Anti-inflammatory activities of ent-16αH,17-hydroxy-kauran-19-oic acid isolated from the roots of Siegesbeckia pubescens are due to the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-κB inactivation[J].Eur J Pharmacol,2007,558(1/2/3):185-193.
[12]王鹏.豨莶草乙醇提取物改善微循环及止痒的研究[J].医药论坛杂志,2003,24(12):19-21.
[13]罗琼,汪建平,阮金兰,等.豨莶草促进皮肤创伤愈合的实验研究[J].医药导报,2008,27(10):1161-1163.
[14]广州部队后勤部,卫生部.常用中草药手册[M].北京:人民卫生出版社,1969.608-609.
[15]魏伟,吴希美,李元建.药理实验方法学[M].北京:人民卫生出版社,2010.639-640.
[16]孙崇恕,孙崇娟.豨莶草治顽固性失眠症[J].实用中西医结合临床,2005,5(5):87.
[17]张哲锋,杨连荣,李彦冰,等.豨莶草化学成分与药理活性的研究进展[J].中医药信息,2006,23(3):15-17.
[18]Xiong J,Ma YB,Xu YL,et al.The constituents of Siegesbeckia orientalis[J].Nat Product Sci,1997,3(1):14-18.
[19]Wang R,Chen WH,Shi YP.Ent-kaurane and ent-pimarane diterpenoids from Siegesbeckia pubescens[J].J Nat Prod,2010,73(1):17-21.
[2]张丽萍,夏猛.失眠症的治疗现状分析及思考[J].环球中医药,2011,4(1):66-69.
[3]赵忠新,张照环.应给予睡眠更多的关注[J].中华神经科杂志,2011,44(8):513-515.
[4]Mallory LJ,Taylor DJ,Lichstein KL,et al.Epidemiology of insomnia and medical disorders[J].SLEEP,2006,29:245-246.
[5]范英兰,张宏,甘雨,等.抗失眠中药复方对KM小鼠戊巴比妥钠催眠作用影响随机平行对照研究[J].实用中医内科杂志,2016,30(9):90-94.
[6]国家药典委员会.中华人民共和国药典-四部:2015年版分辑号:四部[M].北京:中国医药科技出版社,2015.368.
[7]罗琼,汪建平,阮金兰,等.豨莶草局部外用的抗炎镇痛作用研究[J].湖北中医学院学报,2008,10(3):9-11.
[8]胡慧华,汤鲁霞,李小猛.豨莶草生品和炮制品抗炎、抗风湿作用的实验研究[J].中国中药杂志,2004,29(6):542-545.
[9]钱瑞琴,张春英,付宏征.豨莶草活性部位抗风湿作用机理研究[J].中国中西医结合杂志,2000,20(3):192-195.
[10]Kim JY,Lim HJ,Ryu JH.In vitro anti-inflammatory activity of 3-O-methyl-flavones isolated from Siegesbeckia glabrescens[J].Bioorg Med Chem Lett,2008,18(4):1511-1514.
[11]Park HJ,Kim IT,Won JH,et al.Anti-inflammatory activities of ent-16αH,17-hydroxy-kauran-19-oic acid isolated from the roots of Siegesbeckia pubescens are due to the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-κB inactivation[J].Eur J Pharmacol,2007,558(1/2/3):185-193.
[12]王鹏.豨莶草乙醇提取物改善微循环及止痒的研究[J].医药论坛杂志,2003,24(12):19-21.
[13]罗琼,汪建平,阮金兰,等.豨莶草促进皮肤创伤愈合的实验研究[J].医药导报,2008,27(10):1161-1163.
[14]广州部队后勤部,卫生部.常用中草药手册[M].北京:人民卫生出版社,1969.608-609.
[15]魏伟,吴希美,李元建.药理实验方法学[M].北京:人民卫生出版社,2010.639-640.
[16]孙崇恕,孙崇娟.豨莶草治顽固性失眠症[J].实用中西医结合临床,2005,5(5):87.
[17]张哲锋,杨连荣,李彦冰,等.豨莶草化学成分与药理活性的研究进展[J].中医药信息,2006,23(3):15-17.
[18]Xiong J,Ma YB,Xu YL,et al.The constituents of Siegesbeckia orientalis[J].Nat Product Sci,1997,3(1):14-18.
[19]Wang R,Chen WH,Shi YP.Ent-kaurane and ent-pimarane diterpenoids from Siegesbeckia pubescens[J].J Nat Prod,2010,73(1):17-21.