摘要
目的探讨机械加载对骨关节炎(OA)模型小鼠早、晚期OA的治疗作用。方法选用8周龄的C57BL/6雌性小鼠36只,按随机数字表法分为正常对照组(Control组)、OA组和OA机械加载治疗组(OAL组),每组又分为2周和8周亚组(n=6)。使用HE和番红O/快绿染色观察机械加载治疗对早、晚期OA小鼠软骨、滑膜和骨赘的治疗作用。结果与Control组相比,OA小鼠透明软骨(HC)厚度、全部关节软骨(TAC)厚度均变薄,国际骨关节炎研究学会(OARSI)评分、滑膜评分、骨赘评分均升高(均P<0.05)。机械加载治疗后,OAL组HC、TAC厚度均增加,OARSI评分、滑膜评分、骨赘评分均下降(均P<0.05)。相对数据表明,与早期OA小鼠相比,晚期小鼠的病理变化更严重,并且机械加载对晚期OA的治疗效果更好。相关性分析表明,OA早期软骨的退化可能与滑膜炎症有关,而晚期可能与骨赘形成有关。结论机械加载可以改善OA小鼠的病理改变,可以作为一种新型的临床治疗手段。
Objective To investigate the therapeutic effects of mechanical loading on early and late osteoarthritis(OA) of model mice.Methods Thirty-six 8-week-old female C57 BL/6 mice, weighing about 20 g, were divided into the normal control(Control) group, OA group, and OA+loading(OAL) group by a random number table method. Each group was subdivided into 2-week and 8-week sub-groups(n=6). H&E and Safranin O/fast green staining were used to evaluate the therapeutic effects of mechanical loading on cartilage, synovium and osteophyte in early and late OA mice.Results Compared with the Control group, the thickness of hyaline cartilage(HC) and the thickness of all articular cartilage(TAC)were thinner in OA mice, and the OARSI score, synovial score and osteophyte score were significantly increased(all P<0.05). After mechanical loading, the thickness of HC and TAC were increased in OAL group, and OARSI score, synovial score and osteophyte score were decreased(all P<0.05). The relative data suggested that pathological changes were more severe in mice with late OA compared with mice of early OA, and the therapeutic effect of mechanical loading was better for the late OA. Correlation analysis showed that the early cartilage degradation of OA may be related to the synovial inflammation, and the late stage of OA may be related to the osteophyte formation.Conclusion Mechanical loading can improve the pathological changes of osteoarthritis mice, indicating that mechanical loading can be used as a new type of clinical treatment for osteoarthritis.
引文
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