富氢水减轻丝线诱导的SD大鼠妊娠牙龈炎
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摘要
目的:探讨氢气对牙龈炎孕鼠牙龈局部损伤的保护作用及其可能的机制。方法:取SD雌鼠,采用结扎法诱导牙龈炎后,与雄鼠合笼交配。将孕鼠随机分为对照组、造模组和氢水组。对照组和造模组孕鼠饮用纯水,氢水组给予饱和氢水,1天2次。于妊娠第16天处死孕鼠,ELISA法检测各实验组孕鼠牙龈中Prog、SOD、TNF-α的水平,免疫组织化学和Western免疫印迹法检测牙龈中PR、NFκB和TNF-α的表达。采用SPSS13.0软件包对数据进行统计学分析。结果:丝线结扎可诱导SD大鼠妊娠期牙龈炎,PR主要分布于各实验组牙龈上皮细胞的胞质中,孕激素(Prog)及其受体(PR)的水平在各实验组孕鼠牙龈局部无显著差异(P>0.05)。造模组大鼠牙龈内SOD含量显著降低,NFκB和TNF-α表达显著升高。与造模组相比,氢水组孕鼠牙龈炎症反应明显减轻,牙龈内NFκB和TNF-α的表达下调。结论:孕激素及其受体可能在实验大鼠妊娠牙龈炎的发生与发展过程中发挥间接作用,氢气可能通过抑制氧化应激相关炎症反应,减轻实验大鼠的妊娠牙龈炎。
PURPOSE:To investigate the effect of hydrogen rich water on experimental gingivitis in SD rats during pregnancy.METHODS:Female SD rats mated with male ones were chosen to induce experimental gingivitis after ligation for2 weeks.The pregnant rats were randomly divided into control group,model group and HW group.In the control and model group,rats were given pure water,while animals in the HW group were given hydrogen-rich water twice a day.All pregnant animals were sacrificed on day 16 of pregnancy.The level of Prog,SOD and TNF-α in the gingiva of different groups were measured by ELISA,the expression of PR,NFκB and TNF-α were determined by immunohistochemistry and Western blot.SPSS 13.0 software package was used for data analysis.RESULTS:Pregnancy gingivitis of SD rats could be induced by thread ligation.PR was mainly distributed in the gingival epithelium,while there was no significant difference of Prog and PR in the gingiva among different groups(P>0.05).Furthermore,in the model group,lower SOD level as well as higher NFκB and TNF-α level were found in the gingiva.Compared with the model group,the inflammatory response of pregnancy gingivitis in HW group was significantly suppressed along with decreased NFκB and TNF-α.CONCLUSIONS:Progesterone and its receptor may play an indirect role in the process of pregnancy gingivitis of rats.Hydrogen rich water may be beneficial in suppressing pregnancy gingivitis progress by decreasing inflammatory response related to gingival oxidative stress.
PURPOSE:To investigate the effect of hydrogen rich water on experimental gingivitis in SD rats during pregnancy.METHODS:Female SD rats mated with male ones were chosen to induce experimental gingivitis after ligation for2 weeks.The pregnant rats were randomly divided into control group,model group and HW group.In the control and model group,rats were given pure water,while animals in the HW group were given hydrogen-rich water twice a day.All pregnant animals were sacrificed on day 16 of pregnancy.The level of Prog,SOD and TNF-α in the gingiva of different groups were measured by ELISA,the expression of PR,NFκB and TNF-α were determined by immunohistochemistry and Western blot.SPSS 13.0 software package was used for data analysis.RESULTS:Pregnancy gingivitis of SD rats could be induced by thread ligation.PR was mainly distributed in the gingival epithelium,while there was no significant difference of Prog and PR in the gingiva among different groups(P>0.05).Furthermore,in the model group,lower SOD level as well as higher NFκB and TNF-α level were found in the gingiva.Compared with the model group,the inflammatory response of pregnancy gingivitis in HW group was significantly suppressed along with decreased NFκB and TNF-α.CONCLUSIONS:Progesterone and its receptor may play an indirect role in the process of pregnancy gingivitis of rats.Hydrogen rich water may be beneficial in suppressing pregnancy gingivitis progress by decreasing inflammatory response related to gingival oxidative stress.
引文
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[10]Mano Y,Kotani T,Ito M,et al.Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion[J].Free Radic Biol Med,2014,69:324-330.
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[13]史芳川,钟良军,王进涛,等.基质金属蛋白酶3在慢性牙周炎孕鼠模型中的表达[J].口腔医学,2010,30(2):70-72,92.
[14]Irie K,Tomofuji T,Tamaki N,et al.Effects of ethanol consumption on periodontal inflammation in rats[J].J Dent Res,2008,87(5):456-460.
[15]Ni J,Yang D,Song L,et al.Protective effects of paeoniflorin on alveolar bone resorption and soft-tissue breakdown in experimental periodontitis[J].J Periodontal Res,2016,51(2):257-264.
[16]Wei L,Ge L,Qin S,et al.Hydrogen-rich saline protects retina against glutamate-induced excitotoxic injury in guinea pig[J].Exp Eye Res,2012,94(1):117-127.
[17]Song G,Li M,Sang H,et al.Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome[J].J Lipid Res,2013,54(7):1884-1893.
[18]Kasuyama K,Tomofuji T,Ekuni D,et al.Hydrogen-rich water attenuates experimental periodontitis in a rat model[J].J Clin Periodontol,2011,38(12):1085-1090.
[19]孟焕新.牙周病学[M].北京:人民卫生出版社,2007:141.
[20]刘本静,杨美薷.性激素在牙周病中的致病作用[J].国外医学·口腔医学分册,1991,18(1):73-77.
[21]鄢明明,刘泓,冯占军,等.实验性牙周炎雌、孕激素受体免疫组织化学观察[J].哈尔滨医科大学学报,2002,36(1):45-47.
[22]谢云锋,束蓉.雌激素受体和孕激素受体在女性牙周炎患者牙龈组织中的表达[J].上海口腔医学,2003,12(5):366-369.
[23]Figuero E,Carrillo-de-Albornoz A,Herrera D,et al.Gingival changes during pregnancy:I.Influence of hormonal variations on clinical and immunological parameters[J].J Clin Periodontol,2010,37(3):220-229.
[24]Queisser N,Schupp N.Aldosterone,oxidativestress,and NF-k B activation in hypertension-related cardiovascular and renal diseases[J].Free Radic Biol Med,2012,53(2):314-327.
[25]Lee S,Hong J,Choi SY,et al.Cp G oligodeoxynucleotides induce expression of proinflammatory cytokines and chemokines in astrocytes:the role of c-Jun N-terminal kinase in Cp G ODN-mediated NF-kappa B activation[J].J Neuroimmunol,2004,153(1-2):50-63.
[26]Caamao J,Hunter CA.NF-kappa B family of transcription factors:central regulators of innate and adaptive immune functions[J].Clin Microbiol Rev,2002,15(3):414-429.
[27]Furusu A,Nakayama K,Xu Q,et al.MAP kinase-dependent,NF-kappa B-independent regulation of inhibitor of apoptosis protein genes by TNF-alpha[J].J Cell Physiol,2007,210(3):703-710.
[28]Shen M,Zhang H,Yu C,et al.A review of experimental studies of hydrogen as a new therapeutic agent in emergency and critical care medicine[J].Med Gas Res,2014,4:17.
[2]翦新春,刘德裕,徐普.妊娠对牙及牙周组织的影响[J].中华口腔医学研究杂志(电子版),2011,5(6):563-567.
[3]杨丽君.牙周炎与全身性疾病相关性研究[J].中外医学研究,2010,8(17):152-153.
[4]Levy Y.Oxidative stress,antioxidants and periodontal disease[J].Arch Oral Biol,2015,60(9):1461-1462.
[5]Muniz FW,Nogueira SB,Mendes FL,et al.The impact of antioxidant agents complimentary to periodontal therapy on oxidative stress and periodontal outcomes:a systematic review[J].Arch Oral Biol,2015,60(9):1203-1214.
[6]Canakci V,Yildirim A,Canakci CF,et al.Total antioxidant capacity and antioxidant enzymes in serum,saliva,and gingival crevicular fluid of preeclamptic women with and without periodontal disease[J].J Periodontol,2007,78(8):1602-1611.
[7]Gümüs P,Emingil G,ztürk V,et al.Oxidative stress markers in saliva and periodontal disease status:modulation during pregnancy and postpartum[J].BMC Infect Dis,2015,15:261.
[8]Ohsawa I,Ishikawa M,Takahashi K,et al.Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals[J].Nat Med,2007,13(6):688-694.
[9]Huang CS,Kawamura T,Toyoda Y,et al.Recent advances in hydrogen research as a therapeutic medical gas[J].Free Radic Res,2010,44(9):971-982.
[10]Mano Y,Kotani T,Ito M,et al.Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion[J].Free Radic Biol Med,2014,69:324-330.
[11]Hattori Y,Kotani T,Tsuda H,et al.Maternal molecular hydrogen treatment attenuates lipopolysaccharide-induced rat fetal lung injury[J].Free Radic Res,2015,49(8):1026-1037.
[12]Ohta S.Recent progress toward hydrogen medicine:potential of molecular hydrogen for preventive and therapeutic applications[J].Curr Pharm Des,2011,17(22):2241-2252.
[13]史芳川,钟良军,王进涛,等.基质金属蛋白酶3在慢性牙周炎孕鼠模型中的表达[J].口腔医学,2010,30(2):70-72,92.
[14]Irie K,Tomofuji T,Tamaki N,et al.Effects of ethanol consumption on periodontal inflammation in rats[J].J Dent Res,2008,87(5):456-460.
[15]Ni J,Yang D,Song L,et al.Protective effects of paeoniflorin on alveolar bone resorption and soft-tissue breakdown in experimental periodontitis[J].J Periodontal Res,2016,51(2):257-264.
[16]Wei L,Ge L,Qin S,et al.Hydrogen-rich saline protects retina against glutamate-induced excitotoxic injury in guinea pig[J].Exp Eye Res,2012,94(1):117-127.
[17]Song G,Li M,Sang H,et al.Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome[J].J Lipid Res,2013,54(7):1884-1893.
[18]Kasuyama K,Tomofuji T,Ekuni D,et al.Hydrogen-rich water attenuates experimental periodontitis in a rat model[J].J Clin Periodontol,2011,38(12):1085-1090.
[19]孟焕新.牙周病学[M].北京:人民卫生出版社,2007:141.
[20]刘本静,杨美薷.性激素在牙周病中的致病作用[J].国外医学·口腔医学分册,1991,18(1):73-77.
[21]鄢明明,刘泓,冯占军,等.实验性牙周炎雌、孕激素受体免疫组织化学观察[J].哈尔滨医科大学学报,2002,36(1):45-47.
[22]谢云锋,束蓉.雌激素受体和孕激素受体在女性牙周炎患者牙龈组织中的表达[J].上海口腔医学,2003,12(5):366-369.
[23]Figuero E,Carrillo-de-Albornoz A,Herrera D,et al.Gingival changes during pregnancy:I.Influence of hormonal variations on clinical and immunological parameters[J].J Clin Periodontol,2010,37(3):220-229.
[24]Queisser N,Schupp N.Aldosterone,oxidativestress,and NF-k B activation in hypertension-related cardiovascular and renal diseases[J].Free Radic Biol Med,2012,53(2):314-327.
[25]Lee S,Hong J,Choi SY,et al.Cp G oligodeoxynucleotides induce expression of proinflammatory cytokines and chemokines in astrocytes:the role of c-Jun N-terminal kinase in Cp G ODN-mediated NF-kappa B activation[J].J Neuroimmunol,2004,153(1-2):50-63.
[26]Caamao J,Hunter CA.NF-kappa B family of transcription factors:central regulators of innate and adaptive immune functions[J].Clin Microbiol Rev,2002,15(3):414-429.
[27]Furusu A,Nakayama K,Xu Q,et al.MAP kinase-dependent,NF-kappa B-independent regulation of inhibitor of apoptosis protein genes by TNF-alpha[J].J Cell Physiol,2007,210(3):703-710.
[28]Shen M,Zhang H,Yu C,et al.A review of experimental studies of hydrogen as a new therapeutic agent in emergency and critical care medicine[J].Med Gas Res,2014,4:17.