植物非编码RNA及其在甜菜中的应用研究
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摘要
非编码RNA是在真核生物中发现的具有调控功能的RNA,能够以各种方式参与植物的生长发育、信号转导以及逆境胁迫等生命过程。甜菜是我国重要的糖料作物,最近研究发现非编码RNA中的miRNA能够以多种方式参与到甜菜应对盐胁迫、干旱胁迫及养分胁迫的应答中。另外,在病毒侵染甜菜过程中发现非编码RNA起到重要作用。因此研究甜菜非编码RNA对于甜菜的种植和品种改良具有重要意义。本文简要综述了目前研究较多的miRNA、lncRNA和circRNA的产生方式及调控机制,并介绍了miRNA参与甜菜逆境胁迫和lncRNA影响甜菜春化机制的一些研究,旨在对未来甜菜非编码RNA的进一步研究提供理论指导。
Non-coding RNA(ncRNA) is a regulatory RNA found in eukaryotes, which can participate in life processes such as plant growth and development, signal transduction, and stress in various ways. Sugar beet(Beta vulgaris L.) is one of the most important sugar crops in China. Recent studies have found that miRNAs in noncoding RNA could participate in the response to salt stress, drought stress and nutrient stress in a variety of ways in sugar beet. In addition, non-coding RNA is found to play an important role in the process of virus infection of sugar beet. Therefore, the study on non-coding RNA is of great significance for the cultivation and variety improvement in sugar beet. In this review, the production modes and regulatory mechanisms of miRNA, lncRNA and circRNA were briefly summarized, and some researches on the mechanism of miRNA involvement in beet stress and lncRNA affecting the vernalization mechanism of beet were introduced, aiming to provide theoretical guidance for further studies on non-coding RNA of sugar beet in the future.
Non-coding RNA(ncRNA) is a regulatory RNA found in eukaryotes, which can participate in life processes such as plant growth and development, signal transduction, and stress in various ways. Sugar beet(Beta vulgaris L.) is one of the most important sugar crops in China. Recent studies have found that miRNAs in noncoding RNA could participate in the response to salt stress, drought stress and nutrient stress in a variety of ways in sugar beet. In addition, non-coding RNA is found to play an important role in the process of virus infection of sugar beet. Therefore, the study on non-coding RNA is of great significance for the cultivation and variety improvement in sugar beet. In this review, the production modes and regulatory mechanisms of miRNA, lncRNA and circRNA were briefly summarized, and some researches on the mechanism of miRNA involvement in beet stress and lncRNA affecting the vernalization mechanism of beet were introduced, aiming to provide theoretical guidance for further studies on non-coding RNA of sugar beet in the future.
引文
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[21]刘娜,李红侠,张文彬,等.2015年国外甜菊研究回顾[J].中国糖料,2017,39(1):57-64.
[22]Wu M F,Tian Q,Reed J W.Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression,and regulates both female and male reproduction[J].Development,2006,133(21):4211-4218.
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[28]Zhang Y L,Huang Q X,Yin G H,et al.Identification of microRNAs by small RNA deep sequencing for synthetic microRNAmimics to control Spodoptera exigua[J].Gene,2015,557(2):215-221.
[29]Mittal D,Sharma N,Sharma V,et al.Role of microRNAs in rice plant under salt stress[J].Annals of Applied Biology,2016,168(1):2-18.
[30]Lin H H,Tian X,Li Y J,et al.Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana[J].RNA,2008,14(5):836-843.
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[36]Aung K,Lin S I,Wu C C,et al.pho2,a phosphate overaccumulator,is caused by a nonsense mutation in a microRNA399 target gene[J].Plant physiology,2006,141(3):1000-1011.
[37]Kawashima C G,Yoshimoto N,Maruyama-Nakashita,et al.Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types[J].The plant journal:for cell and molecular biology,2009,57(2):313-321.
[38]Yan B,Wang Z H,Guo J T.The research strategies for probing the function of long noncoding RNAs[J].Genomics,2012,99(2):76-80.
[39]Hung T,Chang H Y.Long noncoding RNA ingenome regulation:prospects and mechanisms[J].RNA Biology,2010,7(5):582-585.
[40]St Laurent G,Wahlestedt C,Kapranov P.The Landscape of long noncoding RNA classification[J].Trends in Genetics,2015,31(5):239-251.
[41]Ma H,Hao Y,Dong X,et al.Molecular mechanisms and function prediction of long noncoding RNA[J].Scientific World Journal,2012,2012:541786.
[42]Li S,Yamada M,Han X,et al.High-resolution expression map of the Arabidopsis root reveals alternative splicing and lincRNAregulation[J].Dev Cell,2016,39:508-522.
[43]Zou C,Wang Q,Lu C,et al.Transcriptome analysis reveals long noncoding RNAs involved in fiber development in cotton(Gossypium arboreum)[J].Sci China Life Sci,2016,59(2):164-171.
[44]Li L,Eichten S R,Shimizu R,et al.Genome-wide discovery and characterization of maize long non-coding RNAs[J].Genome Biol,2014,15:R40.
[45]Wen J,Parker B J,Weiller G F.In Silico identification and characterization of mRNA-like noncoding transcripts in Medicago truncatula[J].In Silico Biol,2007,7:485-505.
[46]Hao Z,Fan C,Cheng T,et al.Genome-wide identification,characterization and evolutionary analysis of long intergenic noncoding RNAs in cucumber[J].PLoS One,2015,10(3):e0121800.
[47]Flórez-Zapata N M,Reyes-Valdés M H,Martínez O.Long non-coding RNAs are major contributors to transcriptome changes in sunflower meiocytes with different recombination rates[J].BMC Genomics,2016,17:490.
[48]Csorba T,Uesta J,Sum Q,et al.Antisense COOLAIR mediates the coordinated switching of chromatinstates at FLC during vernalization[J].Proceedings of Naional Academy Science USA,2014,111(45):16160-16165.
[49]Yamaguchi A,Aba M.Regulation of reproductive development by non-coding RNA in Arabidopsis:to flower or not to flower[J].Journal of Plant Rsesarch,2012,125(6):693-704.
[50]Ding J,Lu Q,Ouyang Y,et al.A long noncoding RNA regulates photo period-sensitive male sterility,an essential component of hybrid rice[J].Proc.Natl.Acad.Sci.,USA,2012,109(7):2654-2659.
[51]Wang T Z,Liu M,Zhao M G,et al.Identification and characterization of long non-coding RNAs involved in osmotic and salt stress in Medicago truncatula using genome-wide high-throughput sequencing[J].BMC Plant Biology,2015,15(1):1-13.
[52]王玥,程大友,崔杰,等.植物长链非编码RNA研究进展[J].中国甜菜糖业,2018(3):41-48.
[53]Qin T,Zhao H,Cui P,et al.A nucleus-localized long non-coding RNA enhances drought and salt stress tolerance[J].Plant Physiol,2017,175(3):1321-1336.
[54]Zhang Y,Zhang X,Chen T,et al.Circular introniclong noncoding RNAs[J].Molecular cell,2013,51(16):792.
[55]Salzman J.Circular RNA expression:its potential regulation and function[J].Trends in Genetics,2016,32(5):309-316.
[56]Sanger H L,Klotz G,Riesner D,et al.Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures[J].Proceedings of the National Academy of Sciences,1976,73:3852-3856.
[57]Wang P L,Bao Y,Yee M C,et al.Circular RNA Is Expressed across the Eukaryotic Tree of Life[J].Plos One,2014,9:e90859.
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