中国东南沿海蝙蝠携带细小病毒的初步分子鉴定
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摘要
为了研究中国东南沿海地区蝙蝠作为媒介和宿主携带重要人兽共患病毒的种类及分布情况,分析蝙蝠传播病毒的可能性,从东南沿海地区多个采样点采集了一定数量的成年蝙蝠样本,取部分肠道组织样本进行处理后,用高通量测序和生物信息学对其携带病毒的核酸序列进行了提取和分析。结果显示,蝙蝠携带多种昆虫病毒、植物病毒和脊椎动物病毒,其中细小病毒读长占比最多,15 012个病毒读长中有12 894个是细小病毒序列。针对高通量测序结果,对蝙蝠携带的细小病毒进行了鉴定与分析,并获取了大部分编码区基因,同源比对发现与BtRf-PV病毒株和SX2013株同源性最高,为91%。在采集的268只蝙蝠中,检测到16只携带细小病毒,阳性率为5.9%。
Viruses carried by bats in the southeastern coastal areas of China were screened with next-generation sequencing analysis. The results showed that many viruses were involved, but parvoviruses were the top ones, with a total of 150 812 viral read lengths and 12 894 parvovirus reads. Accordingly, the parvovirus was identified and analyzed with series gene walking amplifications. The coding regions of parvovirus was amplified from bat intestinal tissue, and the homology alignment showed the highest homology with BtRf-PV/SX2013(91%). The preliminary investigations pave the way to uncover the myth of virus carried by bat populations in China.
Viruses carried by bats in the southeastern coastal areas of China were screened with next-generation sequencing analysis. The results showed that many viruses were involved, but parvoviruses were the top ones, with a total of 150 812 viral read lengths and 12 894 parvovirus reads. Accordingly, the parvovirus was identified and analyzed with series gene walking amplifications. The coding regions of parvovirus was amplified from bat intestinal tissue, and the homology alignment showed the highest homology with BtRf-PV/SX2013(91%). The preliminary investigations pave the way to uncover the myth of virus carried by bat populations in China.
引文
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Pereira CA,Monezi TA,Mehnert DU et al.Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay.Vet.Microbiol.,2000,75:127-133.
Phan TG,Vo NP,Bonkoungou IJ et al.Acute diarrhea in West African children:diverse enteric viruses and a novel parvovirus genus.J Virol.,2013,86:11 024-11 030.
Quan PL,Firth C,Conte JM,Williams SH et al.Bats are a major natural reservoir for hepaciviruses and pegiviruses.Proc Natl Acad Sci USA 2013,110:8 194-8 199.
Ryan PA,Martin L,Mackenzie JS,Kay BH.Investigation of gray -headed flying foxes (Pteropus poliocephalus) (Megachiroptera:Pteropodidae) and mosquitoes in the ecology of River virus in Australia.Am J Trop Med Hyg.,1997,57 (4):476-482.
Wu Z,Ren X,Yang L et al.Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces.J Virol.,2012,86:10 999-11 012.
Wu Z,Yang L,Ren X et al.Deciphering the bat virome catalog to better understand the ecological diversity of bat viruses and the bat origin of emerging infectious diseases.ISME Journal,2016,10:609-620.
Zhou T,Zhang HL.Research progress of bat as a host of arbovirus.International Journal of Virology,2009,16(1):1-7.[周涛,张海林.蝙蝠作为虫媒病毒宿主的研究进展.国际病毒学杂志,2009,16(1):1-7.]
Brown KE.The expanding range of parvoviruses which infect humans.Rev Med Virol.,2010,20:231-244.
Calisher CH,Childs JE,Field HE et al.Bats:important reservoir hosts of emerging viruses.Clin Microbiol Rev.,2006,19:531-545.
Canuti M,Eis-Huebinger AM,Deijs M et al.Two novel parvoviruses in frugivorous new and old world bats.PLoS One,2011,6:e29140.
Drosten C,Günther S,Preiser W et al.Identification of a novel coronavirus in patients with severe acute respiratory syndrome.N Engl J Med.,2003,348(20):1 967-1 976.
Guan Y,Zheng BJ,He YQ et al.Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China.Science,2003,302(5 643):276-278.
Hu D,Zhu CQ,Wang Y et al.Virome analysis for identification of novel mammalian viruses in bats from Southeast China.Scienctific Reports,2017,7:10 917.
Jones KE,Patel NG,Levy MA et al.Global trends in emerging infectious diseases.Nature,2008,451:990-993.
Jongenell CV,Sahli R,Mcmaster GK et al.A precise map of splice junctions in the mRNA’s of minute virus of mice,an autonomous parvovirus.J Virol.,1986,59:564-573.
Lau SK,Woo PC,Tse H et al.Identification of novel porcine and bovine parvoviruses closely related to human parvovirus4.J Gen Virol.,2008,89(8):1 840-1 848.
Lau SKP,Ahmed SS,Tsoi HW et al.Bats host diverse parvoviruses as possible origin of mammalian dependoparvoviruses and source for bat-swine interspecies transmission.J Gen Virol,2017,98:3 046-3 059.
Luis AD,Hayman DT,O'Shea TJ et al.A comparison of bats and rodents as reservoirs of zoonotic viruses:are bats special?Proc Biol Sci.,2013,280:20122753.
O'Shea TJ,Cryan PM,Cunningham AA et al.Bat flight and zoonotic viruses.Emerg Infect Dis.,2014,20:741-745.
Pereira CA,Monezi TA,Mehnert DU et al.Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay.Vet.Microbiol.,2000,75:127-133.
Phan TG,Vo NP,Bonkoungou IJ et al.Acute diarrhea in West African children:diverse enteric viruses and a novel parvovirus genus.J Virol.,2013,86:11 024-11 030.
Quan PL,Firth C,Conte JM,Williams SH et al.Bats are a major natural reservoir for hepaciviruses and pegiviruses.Proc Natl Acad Sci USA 2013,110:8 194-8 199.
Ryan PA,Martin L,Mackenzie JS,Kay BH.Investigation of gray -headed flying foxes (Pteropus poliocephalus) (Megachiroptera:Pteropodidae) and mosquitoes in the ecology of River virus in Australia.Am J Trop Med Hyg.,1997,57 (4):476-482.
Wu Z,Ren X,Yang L et al.Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces.J Virol.,2012,86:10 999-11 012.
Wu Z,Yang L,Ren X et al.Deciphering the bat virome catalog to better understand the ecological diversity of bat viruses and the bat origin of emerging infectious diseases.ISME Journal,2016,10:609-620.
Zhou T,Zhang HL.Research progress of bat as a host of arbovirus.International Journal of Virology,2009,16(1):1-7.[周涛,张海林.蝙蝠作为虫媒病毒宿主的研究进展.国际病毒学杂志,2009,16(1):1-7.]