酒精浓度对"饮食偏嗜、饮酒过量"模型大鼠血液黏度等指标的影响
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摘要
目的:观察5周内3种不同酒精浓度对"饮食偏嗜、饮酒过量"模型大鼠血液黏度、血脂、血压等指标的影响。方法:8周龄雄性SD大鼠随机分成5组:普通饲料+水饮组,高盐高脂饲料+水饮组,高盐高脂饲料+5%酒饮组,高盐高脂饲料+7.5%酒饮组,高盐高脂饲料+10%酒饮组。造模第2、5周观察大鼠的全血黏度、血浆黏度和血脂四项;造模第3、5周观察大鼠血压。结果:造模第2、5周,4个造模组大鼠血浆黏度显著升高,高盐高脂饲料复合5%、7.5%酒饮组大鼠全血黏度显著升高;4个复合酒饮造模组大鼠血清TC、LDL-c显著升高,HDL-c显著降低;造模第5周,高盐高脂饲料复合5%、7.5%酒饮组大鼠SBP显著升高。其中以高盐高脂饲料复合7.5%酒饮造模组上述指标变化最明显。结论:酒精浓度对"饮食偏嗜、饮酒过量"模型大鼠血液黏度、血脂、血压等指标有显著的影响,8周龄SD大鼠采用高盐高脂饲料复合7.5%酒饮进行造模,方法最优,可用于高黏血症、高脂血症、高血压等代谢性疾病模型的复制。
Objective: To investigate effects of 3 different concentrations of alcohol on the blood viscosity,the blood lipid,the blood pressure and other indicators in " diet partial preference,drinking excess" model rats within 5 weeks. Methods: 8 weeks old male SD rats were randomly divided into 5 groups: normal diet + water,high salt and high fat diet + water,high salt and high fat diet + 5% liquor,high salt and high fat diet + 7. 5% liquor,high salt and high fat diet + 10% liquor. The whole blood viscosity,plasma viscosity and blood lipids level were observed in the 2 nd and 5 th week. Blood pressures of 8 weeks old rats were observed in the 3 rd and 5 th weeks. Results: Plasma viscosities increased in 4 model rats groups and HDL-c level decreased in the 2 nd and 5 th weeks,whole blood viscosities were significantly elevated in high salt and high fat diet combined with 5% and 7. 5% liquor groups. In addition,levels of TC and LDL-c were significantly increased and HDL-c significantly decreased in 4 model rats groups. SBP of rats in high salt and high fat diet combined with 5% and 7. 5% liquor groups significantly increased in the 5 th week. Among them,there were most obvious changes in above indicators in the high salt and high fat diet combined with 7. 5% liquor group. Conclusion: Different concentrations of alcohol has significant effects on changes of blood viscosity,blood lipid and blood pressure in rats with " diet partial preference,drinking excess". It is most excellent for 8 weeks old rats to adopt the method of high salt and high fat diet combined with 7. 5% liquor,the method is also suitable for the replication of hyperviscosity,hyperlipidemia,hypertension and other metabolic disease models.
Objective: To investigate effects of 3 different concentrations of alcohol on the blood viscosity,the blood lipid,the blood pressure and other indicators in " diet partial preference,drinking excess" model rats within 5 weeks. Methods: 8 weeks old male SD rats were randomly divided into 5 groups: normal diet + water,high salt and high fat diet + water,high salt and high fat diet + 5% liquor,high salt and high fat diet + 7. 5% liquor,high salt and high fat diet + 10% liquor. The whole blood viscosity,plasma viscosity and blood lipids level were observed in the 2 nd and 5 th week. Blood pressures of 8 weeks old rats were observed in the 3 rd and 5 th weeks. Results: Plasma viscosities increased in 4 model rats groups and HDL-c level decreased in the 2 nd and 5 th weeks,whole blood viscosities were significantly elevated in high salt and high fat diet combined with 5% and 7. 5% liquor groups. In addition,levels of TC and LDL-c were significantly increased and HDL-c significantly decreased in 4 model rats groups. SBP of rats in high salt and high fat diet combined with 5% and 7. 5% liquor groups significantly increased in the 5 th week. Among them,there were most obvious changes in above indicators in the high salt and high fat diet combined with 7. 5% liquor group. Conclusion: Different concentrations of alcohol has significant effects on changes of blood viscosity,blood lipid and blood pressure in rats with " diet partial preference,drinking excess". It is most excellent for 8 weeks old rats to adopt the method of high salt and high fat diet combined with 7. 5% liquor,the method is also suitable for the replication of hyperviscosity,hyperlipidemia,hypertension and other metabolic disease models.
引文
1洪鑫芳,贺媛,李红,等.饮酒与血压、血糖、血尿酸及血脂的关系研究.实用预防医学,2016;23(3)∶261~263
2 潘丹丹,高建莉,陈素红,等.高盐高脂酒饮复合因素模拟"饮食不节"对大鼠血液黏度的影响.中国中药杂志,2015;40(8)∶1560~1564
3 郭丽娃,黄健.《诸病源候论》饮食致病说对中医病因学的贡献.北京中医药,2009;28(8)∶599~601
4 李小可,刘灿,赵丹丹,等.饮食偏嗜的成瘾性及病机规律探析.中医杂志,2015;56(10)∶816~820
5 覃光辉,王骁,杨亚魁,等.高黏滞血症从痰论治初探.中医药导报,2007;13(10)∶7~8
6 马冠生,朱丹红,胡小琪,等.中国居民饮酒行为现况.营养学报,2005;27(5)∶362~365
7 冯凤英,张晓燕,张俊丽.长期过量饮酒者戒酒前后血脂变化.包头医学院学报,2013;29(1)∶41~42
8 严家荣,王弋,刘盛来,等.2种高脂血症大鼠模型比较.中国药业,2017;26(8)∶5~7
9 倪兆成,梁慧,王楠楠,等.疏肝调脂方对高脂血症模型大鼠血脂、肝肾功能及微循环的影响.中国现代应用药学,2016;33(4)∶395~399
10 李婕,严士海,王欣彤,等.基于高盐诱导的SHR大鼠肾损伤探讨中医理论"盐胜血"的机理研究.南京中医药大学学报,2016;32(3)∶287 ~290
11 赵秀梅,刘育英,汪德清.高脂血症大鼠血液流变学及肝脏微循环的改变.中国微循环,2006;10(6)∶410~412
12 丁书文,史红霞,陈雨振,等.白酒对大鼠血栓形成影响的实验研究.中国预防医学杂志,2003;4(4)∶249~252
13 高俊凤,赵敏,吴祖舜.纤维蛋白原对血液流变学的影响.脑与神经疾病杂志,2003;11(3)∶183
14 李云霞,蔡久英.饮酒与心血管疾病.心血管病学进展,2008;29(3)∶462~465
15 郑立华,王秋萍,耿红艳,等.血液黏度与血球压积、血糖、血脂和高、低密度脂蛋白的相关性分析.中国实验诊断学,2010;14(9)∶1415~1417
16 Brun J F,Varlet-marie E,Fedou C,et al.Body composition and exercise performance as determinants of blood rheology in middle-aged patients exhibiting the metabolic syndrome.Clinical hemorheology and microcirculation,2011;49(1-4)∶215~223
17 庄华琴,吴琦珀,薛启云,等.酒精依赖患者血流变学指标观察与分析.检验医学与临床,2013;10(5)∶581
18 刘久波,陈林,何晓阔,等.血脂与血液流变性的关系及其对高血压的影响.微循环学杂志,2006;16(3)∶47~48
19 Sloop G D,Weidman J J,St Cyr J A.The systemic vascular resistance response:a cardiovascular response modulating blood viscosity with implications for primary hypertension and certain anemias.Therapeutic advances in cardiovascular disease,2015;9(6)∶403~411
2 潘丹丹,高建莉,陈素红,等.高盐高脂酒饮复合因素模拟"饮食不节"对大鼠血液黏度的影响.中国中药杂志,2015;40(8)∶1560~1564
3 郭丽娃,黄健.《诸病源候论》饮食致病说对中医病因学的贡献.北京中医药,2009;28(8)∶599~601
4 李小可,刘灿,赵丹丹,等.饮食偏嗜的成瘾性及病机规律探析.中医杂志,2015;56(10)∶816~820
5 覃光辉,王骁,杨亚魁,等.高黏滞血症从痰论治初探.中医药导报,2007;13(10)∶7~8
6 马冠生,朱丹红,胡小琪,等.中国居民饮酒行为现况.营养学报,2005;27(5)∶362~365
7 冯凤英,张晓燕,张俊丽.长期过量饮酒者戒酒前后血脂变化.包头医学院学报,2013;29(1)∶41~42
8 严家荣,王弋,刘盛来,等.2种高脂血症大鼠模型比较.中国药业,2017;26(8)∶5~7
9 倪兆成,梁慧,王楠楠,等.疏肝调脂方对高脂血症模型大鼠血脂、肝肾功能及微循环的影响.中国现代应用药学,2016;33(4)∶395~399
10 李婕,严士海,王欣彤,等.基于高盐诱导的SHR大鼠肾损伤探讨中医理论"盐胜血"的机理研究.南京中医药大学学报,2016;32(3)∶287 ~290
11 赵秀梅,刘育英,汪德清.高脂血症大鼠血液流变学及肝脏微循环的改变.中国微循环,2006;10(6)∶410~412
12 丁书文,史红霞,陈雨振,等.白酒对大鼠血栓形成影响的实验研究.中国预防医学杂志,2003;4(4)∶249~252
13 高俊凤,赵敏,吴祖舜.纤维蛋白原对血液流变学的影响.脑与神经疾病杂志,2003;11(3)∶183
14 李云霞,蔡久英.饮酒与心血管疾病.心血管病学进展,2008;29(3)∶462~465
15 郑立华,王秋萍,耿红艳,等.血液黏度与血球压积、血糖、血脂和高、低密度脂蛋白的相关性分析.中国实验诊断学,2010;14(9)∶1415~1417
16 Brun J F,Varlet-marie E,Fedou C,et al.Body composition and exercise performance as determinants of blood rheology in middle-aged patients exhibiting the metabolic syndrome.Clinical hemorheology and microcirculation,2011;49(1-4)∶215~223
17 庄华琴,吴琦珀,薛启云,等.酒精依赖患者血流变学指标观察与分析.检验医学与临床,2013;10(5)∶581
18 刘久波,陈林,何晓阔,等.血脂与血液流变性的关系及其对高血压的影响.微循环学杂志,2006;16(3)∶47~48
19 Sloop G D,Weidman J J,St Cyr J A.The systemic vascular resistance response:a cardiovascular response modulating blood viscosity with implications for primary hypertension and certain anemias.Therapeutic advances in cardiovascular disease,2015;9(6)∶403~411