自发性基因突变SCD1~(-/-)小鼠主要脏器参数、血液生理生化指标的观察
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摘要
目的探讨SCD1基因缺失对小鼠脏器参数,血液生理生化指标的影响。方法选取8周龄SCD1~(-/-)小鼠及其杂合子SCD1~(+/-)小鼠,称取体质量及主要脏器,观察其脏器系数。比较不同周龄SCD1~(-/-)小鼠及其杂合子SCD1~(+/-)小鼠的血液细胞学和生化指标,观察其变化特点。结果 8周龄SCD1~(-/-)小鼠与杂合子小鼠比较肝脏系数显著升高(P<0.05)。6～8周龄SCD1~(-/-)小鼠与杂合子小鼠的血液细胞学指标相比RBC、MPV、PCT显著降低(P<0.05),MCV、MCH、RDW、PDW、PLT、LYM显著增高(P<0.05);血清生化指标ALB、TC、TG极显著降低(P<0.01),ALP、ALT、UA极显著增高(P<0.01)。16～18周龄SCD1~(-/-)小鼠与杂合子小鼠的血液细胞学指标MCHC、PLT、PCT相比极显著降低(P<0.01),RDW、HCT、MCV都极显著增高(P<0.01);血清生化指标ALP、TC极显著降低(P<0.01)。结论 SCD1基因缺失对小鼠鼠脏器参数、血液生理生化指标都有一定的影响。
Objective To investigate the effect of SCD1 gene deletion on organ coefficients and hematology indexes of mice. Method Organ coefficients are observed in 8 weeks SCD1~(-/-) mice and heterozygous SCD1 mice as well as hematology indexes of different ages mice are compared between the two group. Result Liver coefficient of 6-8 weeks SCD1~(-/-)mice markedly increase compared with heterozygous mice. RBC,MPV,PCT of SCD1~(-/-)mice are significantly lower(P<0.05),while MCV,MCH,RDW,PDW,PLT,LYM are remarkbly higher(P<0.05) than those of the control group. ALB,TC,TG also obviously go down and ALP,ALT,UA clearly go up(P<0.01)in 6-8 weeks experiment group. MCHC, PLT, PCT,ALP, TC of 16-18 weeks significantly decrease compared with those of SCD1~(+/-)mice. At the same time,RDW、HCT and MCV significantly increases(P<0.01). Conclusion SCD1 gene deletion impairs the organ coefficients of mice. Moreover, gene SCD1 deletion has impact on some hematology indexes.
Objective To investigate the effect of SCD1 gene deletion on organ coefficients and hematology indexes of mice. Method Organ coefficients are observed in 8 weeks SCD1~(-/-) mice and heterozygous SCD1 mice as well as hematology indexes of different ages mice are compared between the two group. Result Liver coefficient of 6-8 weeks SCD1~(-/-)mice markedly increase compared with heterozygous mice. RBC,MPV,PCT of SCD1~(-/-)mice are significantly lower(P<0.05),while MCV,MCH,RDW,PDW,PLT,LYM are remarkbly higher(P<0.05) than those of the control group. ALB,TC,TG also obviously go down and ALP,ALT,UA clearly go up(P<0.01)in 6-8 weeks experiment group. MCHC, PLT, PCT,ALP, TC of 16-18 weeks significantly decrease compared with those of SCD1~(+/-)mice. At the same time,RDW、HCT and MCV significantly increases(P<0.01). Conclusion SCD1 gene deletion impairs the organ coefficients of mice. Moreover, gene SCD1 deletion has impact on some hematology indexes.
引文
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[2] 章金涛,王纯耀,康巧珍,等. 豫医无毛小鼠近交系建立及其生物学特性[J].上海实验动物科学,1999,19(1):64-65.
[3] 李昊,张西正.小鼠骨质疏松模型建立方法与评价指标选择概述[J].中国骨质疏松杂志,2012,18(7):663-666.
[4] 谭巧燕,钟海潮,刘盛来,等.SPF级与普通级Hartley豚鼠主要脏器参数的比较[J].实验动物科学,2013,30(6): 31-33.
[5] 侯岩岩,史深,麦丽开,等.普通级与清洁级灰仓鼠脏器重量与脏器系数的比较[J].实验动物与比较医学,2011,6(11): 450-452.
[6] 张晓玉,张修彦,陈沃勋,等.SPF级FMMU白化豚鼠基础生理指标的测定与比较[J].实验动物科学,2015,4(32):31-35.
[7] 施新猷,王四旺,顾为望,等.比较医学[M].西安: 陕西科学技术出版社,2003. 104-105.
[8] 尤金炜,张立波,方天,等.绿色荧光裸鼠血液生理生化指标监测分析[J].中国比较医学杂志,2013,(9):23-26.
[9] 孙静,杜蕾,丁玉春,等.无菌猪生长、血液以及脏器相关指标的测定[J].中国实验动物学报,2016,4(24):388-394.