犬细小病毒2型变异株对高母源抗体犬的致病性
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摘要
为了探究CPV-2变异株的致病性,用CPV-2a和CPV-2b野毒株,分别攻击犬细小病毒(CPV-2)高母源抗体(MDA)幼犬,根据犬临床症状,组织病理学,粪便排毒和血清抗体应答等指标,评价高母源抗体(HI滴度≥1∶160)对不同CPV病毒变异株的保护作用。结果表明, CPV-2a和CPV-2b攻毒后引起明显临床症状,攻毒后3～6 d粪便中CPV排毒。感染犬的组织样品经PCR检测表明,CPV-2a/2b病毒广泛分布。组织病理学分析表明,CPV-2a/2b感染引起肠道黏膜出血。研究表明,CPV-2高母源抗体对CPV-2a/2b变异株攻击不能提供有效保护,有必要开发CPV变异株的新型疫苗。
To investigate the pathogenicity of these CPV-2 variants,the puppies with high-titer maternally derived antibody(MDA) to CPV were challenged with CPV variants CPV-2 a and CPV-2 b,respectively.Clinical signs,histopathological changes,virus shedding and antibody responses were detected to evaluate the protection potency of the high-titer MDA(haemagglutination inhibiting(HI) titer≥ 1∶160)against different CPV variants.In comparison with the puppies challenged with CPV-2,the puppies challenged with CPV-2 a and CPV-2 b showed obvious clinical signs and shed viruses in feces from day 3 to day 6 post challenge.Moreover,CPV-2 a and CPV-2 b viruses could be detected by PCR with wide distribution in the tissues.The histopathological analysis further showed that the intestine mucosal hemorrhage could be found in puppies infected with CPV-2 a and CPV-2 b.This finding demonstrate that high-titer MDA to CPV-2 could not effectively protect puppies from CPV-2 a/2 b infection,calling for novel CPV vaccines against CPV-2 variants.
To investigate the pathogenicity of these CPV-2 variants,the puppies with high-titer maternally derived antibody(MDA) to CPV were challenged with CPV variants CPV-2 a and CPV-2 b,respectively.Clinical signs,histopathological changes,virus shedding and antibody responses were detected to evaluate the protection potency of the high-titer MDA(haemagglutination inhibiting(HI) titer≥ 1∶160)against different CPV variants.In comparison with the puppies challenged with CPV-2,the puppies challenged with CPV-2 a and CPV-2 b showed obvious clinical signs and shed viruses in feces from day 3 to day 6 post challenge.Moreover,CPV-2 a and CPV-2 b viruses could be detected by PCR with wide distribution in the tissues.The histopathological analysis further showed that the intestine mucosal hemorrhage could be found in puppies infected with CPV-2 a and CPV-2 b.This finding demonstrate that high-titer MDA to CPV-2 could not effectively protect puppies from CPV-2 a/2 b infection,calling for novel CPV vaccines against CPV-2 variants.
引文
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[5] MOON H S,LEE S A,LEE S G,et al.Comparison of the pathogenicity in three different Korean canine parvovirus 2 (CPV-2) isolates[J].Vet Microbiol,2008,131: 47-56.
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[7] WANG J,LIN P,ZHAO H,et al.Continuing evolution of canine parvovirus in China:Isolation of novel variants with an Ala5Gly mutation in the VP2 protein[J].Infect Genet Evol,2016,38:73-78.
[8] HERNANDEZ-BLANCO B,CATALA-LOPEZ F .Are licensed canine parvovirus (CPV2 and CPV2b) vaccines able to elicit protection against CPV2c subtype in puppies?:A systematic review of controlled clinical trials[J].Vet Microbiol,2015,180(1/2):1-9.
[9] VAN NGUYEN S,UMEDA K,YOKOYAMA H,et al.Passive protection of dogs against clinical disease due to Canine parvovirus-2 by specific antibody from chicken egg yolk[J].Can J Vet Res,2006,70:62-64.
[10] GLOVER S,ANDERSON C,PIONTKOWSKI M,et al.Canine parvovirus (CPV) type 2b vaccine protects puppies with maternal antibodies to CPV when challenged with virulent CPV-2c virus[J].Int J Appl Res Vet Med,2012,10(3):217-224.
[11] ELIA G,CAVALLI A,CIRONE F,et al.Antibody levels and protection to canine parvovirus type 2[J].J Vet Med,2005,52:320-322.
[12] BUONAVOGLIA C,MARTELLA V,PRATELLI A,et al.Evidence for evolution of canine parvovirus type 2 in Italy[J].J Gen Virol,2001,82:3021-3025.
[13] PRATELLI A,CAVALLI A,MARTELLA V,et al.Canine parvovirus (CPV) vaccination:comparison of neutralizing antibody responses in pups after inoculation with CPV2 or CPV2b modified live virus vaccine[J].Clin Diagn Lab Immun,2001,8(3):612-615.
[14] DECARO N,CAMPOLO M.,DESARIO C,et al.Maternally-derived antibodies in pups and protection from canine parvovirus infection[J].Biological,2005,33:261-267.
[15] MEUNIER P C,COOPER B J,APPEL M.J G,et al.Pathogenesis of canine parvovirus enteritis:the importance of viremia[J].Vet Pathol,1985,22:60-71.
[16] DECARO N,BUONAVOGLIA C.Canine parvovirus—A review of epidemiological and diagnostic aspects,with emphasis on type 2c[J].Vet Microbiol,2012,155:1-12.
[17] GODDARD A,LEISEWITZ A L,CHRISTOPHER M M,et al.Prognostic usefulness of blood leukocyte changes in canine parvoviral enteritis[J].J Vet Intern Med,2008,22:309-316
[18] YULE T D,ROTH M B,DREIER K,et al.Canine parvovirus vaccine elicits protection from the inflammatory and clinical consequences of the disease[J].Vaccine, 1997,15:720-729
[19] SPIBEY N,GREENWOOD N M,SUTTON D,et al.Canine parvovirus type 2 vaccine protects against virulent challenge with type 2c virus[J].Vet Microbiol,2008,128:48-55.