动物双歧杆菌AD011表面脂蛋白的全基因组预测和功能分析
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摘要
[目的]运用生物信息学方法预测动物双歧杆菌AD011定位于细胞膜的表面脂蛋白,为后续研究该菌与宿主相互作用的分子机制提供基础资料。[方法]用Signal P 4.0和Lipo P软件对动物双歧杆菌AD011全基因组编码蛋白中表面脂蛋白和信号肽进行预测,同时采用COG功能数据库对预测的脂蛋白进行功能注释和聚类分析。[结果]对动物双歧杆菌AD011基因组1 518个蛋白的表面脂蛋白预测,结果表明,19个蛋白具有脂蛋白信号肽。功能分析显示,该菌株19个脂蛋白中有3个蛋白没有功能注释,16个蛋白有功能注释,其中10个蛋白参与氨基酸代谢与转运、3个蛋白参与碳水化合物代谢与转运,3个蛋白参与无机盐离子代谢与转运。[结论]动物双歧杆菌AD011表面脂蛋白参与的功能主要与营养物质的转运和代谢有关。
[Objective] The surface lipoproteins of Bifidobacterium animalis AD011 were predicted by bioinformatics method,which provided the basis for interaction molecular mechanisms between bacteria and their host. [Method] Signal P 4. 0 and Lipo P softwares were used to analyze the lipoproteins and characteristic of signal peptides. Function of lipoproteins was also analyzed by COG database. [Result]There were 19 proteins which possessed signal peptide of lipoproteins from 1 518 proteins coded by B. animalis AD011 genome. Function analysis revealed that the strain contains 3 hypothetical proteins without function annonation. 16 proteins have function annonation which mainly involved in amino acid metabolism and transport( 10 proteins),transport of carbohydrate metabolism( 3 proteins),metabolism of inorganic ion transport( 3proteins). [Conclusion] Function of surface lipoproteins of B. animalis AD011 are mainly associated with the transport and metabolism of nutrients.
[Objective] The surface lipoproteins of Bifidobacterium animalis AD011 were predicted by bioinformatics method,which provided the basis for interaction molecular mechanisms between bacteria and their host. [Method] Signal P 4. 0 and Lipo P softwares were used to analyze the lipoproteins and characteristic of signal peptides. Function of lipoproteins was also analyzed by COG database. [Result]There were 19 proteins which possessed signal peptide of lipoproteins from 1 518 proteins coded by B. animalis AD011 genome. Function analysis revealed that the strain contains 3 hypothetical proteins without function annonation. 16 proteins have function annonation which mainly involved in amino acid metabolism and transport( 10 proteins),transport of carbohydrate metabolism( 3 proteins),metabolism of inorganic ion transport( 3proteins). [Conclusion] Function of surface lipoproteins of B. animalis AD011 are mainly associated with the transport and metabolism of nutrients.
引文
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[2]LEE J H,O’SULLIVAN D J.Genomic insights into bifidobacteria[J].Microbiology and molecular biology reviews,2010,74(3):378-416.
[3]GLEINSER M,GRIMM V,ZHURINA D,et al.Improved adhesive properties of recombinant bifidobacteria expressing the Bifidobacterium bifidumspecific lipoprotein Bop A[J].Microbial cell factories,2012,11:80.
[4]GUGLIELMETTI S,TAMAGNINI I,MORA D,et al.Implication of an outer surface lipoprotein in adhesion of Bifidobacterium bifidum to Caco-2 cells[J].Applied and environmental microbiology,2008,74(15):4695-4702.
[5]TOBA T,VIRKOLA R,WESTERLUND B,et al.A collagen-binding s-layer protein in Lactobacillus crispatus[J].Applied and environmental microbiology,1995,61(7):2467-2471.
[6]BABU M M,PRIYA M L,SELVAN A T,et al.A database of bacterial lipoproteins(DOLOP)with functional assignments to predicted lipoproteins[J].Journal of bacteriology,2006,188(8):2761-2773.
[7]BARINOV A,LOUX V,HAMMANI A,et al.Prediction of surface exposed proteins in Streptococcus pyogenes,with a potential application to other Gram-positive bacteria[J].Proteomics,2009,9(1):61-73.
[8]RUIZ L,DELGADO S,RUAS-MADIEDO P,et al.Proteinaceous molecules mediating Bifidobacterium-host interactions[J].Frontiers in microbiology,2016,7:1193.
[9]GILAD O,HJERNO K,OSTERLUND E C,et al.Insights into physiological traits of Bifidobacterium animalis subsp.lactis BB-12 through membrane proteome analysis[J].Journal of proteomics,2012,75(4):1190-1200.
[10]GILAD O,SVENSSON B,VIBORG A H,et al.The extracellular proteome of Bifidobacterium animalis subsp.lactis BB-12 reveals proteins with putative roles in probiotic effects[J].Proteomics,2011,11(12):2503-2514.
[11]MILANI C,DURANTI S,LUGLI G A,et al.Comparative genomics of Bifidobacterium animalis subsp.lactis reveals a strict monophyletic bifidobacterial taxon[J].Applied and environmental microbiology,2013,79(14):4304-4315.