UPLC法研究甘草京大戟反药对配伍后京大戟对甘草中有效成分含量的影响
|
摘要
目的采用UPLC法,建立甘草中甘草苷、芹糖甘草苷、刺芒柄花苷、甘草素、甘草酸的含量测定方法,研究甘草单煎液与甘草京大戟不同配伍比例合煎液甘草中5种成分的变化规律,从化学物质基础层面,初步阐述甘草京大戟的配伍禁忌机制。方法制备甘草单煎液与甘草京大戟不同配伍比例合煎液,采用Kromat Universil XB C_8(150 mm×2.1 mm,1.8μm)色谱柱,体积分数为0.1%的甲酸水溶液为流动相A,甲醇为流动相B,梯度洗脱。检测波长:263 nm,进样量:2μL,柱温:35℃,流速:0.2 mL·min~(-1),依次进样分析。结果甘草苷、芹糖甘草苷、刺芒柄花苷、甘草素和甘草酸质量浓度分别在14.50～464.00、27.40～508.30、1.50～49.20、0.98～31.20、30.20～965.20 mg·L~(-1)内与峰面积呈良好的线性关系,相关系数分别为0.999 4、0.999 0、0.999 4、0.999 5、0.999 8;甘草京大戟(质量比为1∶1)合煎液中,5种成分的平均回收率分别为98.7%、97.9%、96.4%、97.0%和99.8%;RSD分别为1.1%、1.6%、1.7%、1.8%和1.5%(n=9)。结论所建立的方法适用于研究十八反中甘草京大戟反药对的配伍变化规律。
Objective To establish a method for the determination of the contents of Liquiritin,Liquiritin apioside,Ononin,Liquiritigenin and Glycyrrhizic Acid in Glycyrrhiza uralensis by UPLC.To describe the incompatibility mechanism in the combination of Glycyrrhiza uralensis Fisch. and Euphorbia pekinensis Rupr. from the basic chemical substances.Methods Glycyrrhiza uralensis Fisch. and combinations of Glycyrrhiza uralensis Fisch. with Euphorbia pekinensis Rupr. at different compatibility ratios were prepared.The separation was performed on a Kromat Universe XB C8(150 mm × 2.1 mm,1.8 μm) column,0.1% formic acid as the mobile phase A,methanol as the mobile phase B,Gradient elution.Detection wavelength:263 nm;injection volume:2 μL;column temperature:35 ℃;flow rate:0.2 mL·min~(-1).In this case,samples were analyzed sequentially.Results The calibration curve was linear within the range of 14.50-464.00 mg·L~(-1)(r=0.999 4),27.40-508.30 mg·L~(-1)(r=0.999 0),1.50-49.20 mg·L~(-1)(r=0.999 4),0.98-31.20 mg·L~(-1)(r=0.999 5),30.20-965.20 mg·L~(-1)(r=0.999 8) for Liquiritin,Liquiritin apioside,Ononin,Liquiritigenin and Glycyrrhizic acid,respectively.In combination of Glycyrrhiza uralensis Fisch. with Euphorbia pekinensis Rupr.(m∶m=1∶1),the average recoveries were 94.3%(RSD=1.4%),94.2%(RSD=1.3%),92.0%(RSD=0.77%),90.7%(RSD=1.7%) and 94.9%(RSD=1.0%) for liquiritin,liquiritin apioside,ononin,liquiritigenin and glycyrrhizic acid,respectively.Conclusion The established method is reliable,simple and reproducibility,which is suitable for study the variation of the combination of Glycyrrhiza uralensis Fisch. with Euphorbia pekinensis Rupr. at different compatibility ratios in eighteen incompatible pairs.
Objective To establish a method for the determination of the contents of Liquiritin,Liquiritin apioside,Ononin,Liquiritigenin and Glycyrrhizic Acid in Glycyrrhiza uralensis by UPLC.To describe the incompatibility mechanism in the combination of Glycyrrhiza uralensis Fisch. and Euphorbia pekinensis Rupr. from the basic chemical substances.Methods Glycyrrhiza uralensis Fisch. and combinations of Glycyrrhiza uralensis Fisch. with Euphorbia pekinensis Rupr. at different compatibility ratios were prepared.The separation was performed on a Kromat Universe XB C8(150 mm × 2.1 mm,1.8 μm) column,0.1% formic acid as the mobile phase A,methanol as the mobile phase B,Gradient elution.Detection wavelength:263 nm;injection volume:2 μL;column temperature:35 ℃;flow rate:0.2 mL·min~(-1).In this case,samples were analyzed sequentially.Results The calibration curve was linear within the range of 14.50-464.00 mg·L~(-1)(r=0.999 4),27.40-508.30 mg·L~(-1)(r=0.999 0),1.50-49.20 mg·L~(-1)(r=0.999 4),0.98-31.20 mg·L~(-1)(r=0.999 5),30.20-965.20 mg·L~(-1)(r=0.999 8) for Liquiritin,Liquiritin apioside,Ononin,Liquiritigenin and Glycyrrhizic acid,respectively.In combination of Glycyrrhiza uralensis Fisch. with Euphorbia pekinensis Rupr.(m∶m=1∶1),the average recoveries were 94.3%(RSD=1.4%),94.2%(RSD=1.3%),92.0%(RSD=0.77%),90.7%(RSD=1.7%) and 94.9%(RSD=1.0%) for liquiritin,liquiritin apioside,ononin,liquiritigenin and glycyrrhizic acid,respectively.Conclusion The established method is reliable,simple and reproducibility,which is suitable for study the variation of the combination of Glycyrrhiza uralensis Fisch. with Euphorbia pekinensis Rupr. at different compatibility ratios in eighteen incompatible pairs.
引文
[1] 陈夏娟,徐立,余果.海藻、芫花反甘草的研究进展[J].中草药,2012,43(5):1024-1027.
[2] 赵桐,钟赣生,张建美,等.含甘遂-甘草反药组合的甘遂半夏汤临床用药分析[J].中国临床医生杂志,2014(12):89-91.
[3] 高晓山,陈馥馨,刘林祥,等.中药十八反的新涵义-妨害治疗[J].中国中药杂志,1992,17(12):754-756,761.
[4] 项妤,刘春生,刘勇,等.甘草中主要活性成分和内源激素含量的动态变化规律研究[J].北京中医药大学学报,2015,38(5):339-343.
[5] 乔湜,李清艳,孙瑞芳,等.复方甘草口服液中7种成分的UPLC-MS /MS分段离子切换法测定[J].中国医药工业杂志,2014,45(5):470-473.
[6] 于辉,李春香,宫凌涛,等.甘草的药理作用概述[J].现代生物医学进展,2006,6(4):77-79.
[7] 刘卉,陈乐天,恽菲,等.甘草吸收代谢特征与机制及其配伍用药相互影响研究与思考[J].中草药,2012,43(7):1443-1447.
[8] 吴宗耀,牛李义,梁喜爱.甘草化学成分及药理作用分析 [J].河南中医,2010,30(12):1235-1236.
[9] 王文萍,王垂杰,谷松,等.芍药甘草汤配伍意义的药动学研究[J].世界科学技术:中医药现代化,2009,11(3):382-387.
[10] 肇鑫宇,金希儒,陈雁飞,等.京大戟醋制前后UPLC指纹图谱及含量测定的比较[J].沈阳药科大学学报,2016,33(11):861-869.
[11] 李向荣.近10年来对中药十八反毒理及其物质基础的研究进展[J].环球中医药,2011,04(4):317-320.
[12] 刘佳,费宇彤,钟赣生,等.十八反中甘草大戟反药组合临床同用文献的文献特征分析[J].中华中医药杂志,2014(5):1691-1695.
[13] 张腾,梁策,佟继铭,等.大戟反甘草的研究进展[J].承德医学院学报,2016,33(3):241-243.
[2] 赵桐,钟赣生,张建美,等.含甘遂-甘草反药组合的甘遂半夏汤临床用药分析[J].中国临床医生杂志,2014(12):89-91.
[3] 高晓山,陈馥馨,刘林祥,等.中药十八反的新涵义-妨害治疗[J].中国中药杂志,1992,17(12):754-756,761.
[4] 项妤,刘春生,刘勇,等.甘草中主要活性成分和内源激素含量的动态变化规律研究[J].北京中医药大学学报,2015,38(5):339-343.
[5] 乔湜,李清艳,孙瑞芳,等.复方甘草口服液中7种成分的UPLC-MS /MS分段离子切换法测定[J].中国医药工业杂志,2014,45(5):470-473.
[6] 于辉,李春香,宫凌涛,等.甘草的药理作用概述[J].现代生物医学进展,2006,6(4):77-79.
[7] 刘卉,陈乐天,恽菲,等.甘草吸收代谢特征与机制及其配伍用药相互影响研究与思考[J].中草药,2012,43(7):1443-1447.
[8] 吴宗耀,牛李义,梁喜爱.甘草化学成分及药理作用分析 [J].河南中医,2010,30(12):1235-1236.
[9] 王文萍,王垂杰,谷松,等.芍药甘草汤配伍意义的药动学研究[J].世界科学技术:中医药现代化,2009,11(3):382-387.
[10] 肇鑫宇,金希儒,陈雁飞,等.京大戟醋制前后UPLC指纹图谱及含量测定的比较[J].沈阳药科大学学报,2016,33(11):861-869.
[11] 李向荣.近10年来对中药十八反毒理及其物质基础的研究进展[J].环球中医药,2011,04(4):317-320.
[12] 刘佳,费宇彤,钟赣生,等.十八反中甘草大戟反药组合临床同用文献的文献特征分析[J].中华中医药杂志,2014(5):1691-1695.
[13] 张腾,梁策,佟继铭,等.大戟反甘草的研究进展[J].承德医学院学报,2016,33(3):241-243.