钩虫卵长期保存方法的研究
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摘要
目的探讨一种简便并可长期保存薄壳寄生虫卵标本的制作方法。方法盐腌含钩虫卵粪便,以5%分级浓度粗筛,每3个月观察虫卵变化,连续3年;取保存效果最好的浓度,再以1%分级浓度配制保存并定期观察保存效果,找到最适宜的浓度。结果第一轮粗筛,35%的浓度保存的效果好。第二轮按增减1%梯度保存,结果是36%浓度保存的虫卵效果最好,3年存放后4个浅灰色分裂的卵细胞清晰明显,壳完整光滑清晰,卵壳与卵细胞之间的空隙稍大,未发现继续发育,无皱缩变形,有利于标本长期保存。结论盐腌固定钩虫卵的保存方法简便易行,值得推广应用。
Objective To develop a long-term preservation method for thin shell parasite eggs. Methods Feces with hookworm eggs were preserved at different concentration of salt(5% grading),observed the changes in eggs every three months for three consecutive years. The best salt concentration was chosen for fine tuning of the salt concentration.Hookworm eggs were preserved with different salt concentration at 1% grading in this range. Results By first round of screening,35% salt was a good concentration for preserving the hookworm eggs. The second round fine tuned study indicated the optimal salt concentration was 36%. After three years,the preserved egg could be hatched and split into four;the egg shell was intact;the lacuna between egg shell and egg was a little larger;the egg stopped development and had no distortion. Conclusion Hookworm eggs could be preserved with salt and still maintained their variability for long time.
Objective To develop a long-term preservation method for thin shell parasite eggs. Methods Feces with hookworm eggs were preserved at different concentration of salt(5% grading),observed the changes in eggs every three months for three consecutive years. The best salt concentration was chosen for fine tuning of the salt concentration.Hookworm eggs were preserved with different salt concentration at 1% grading in this range. Results By first round of screening,35% salt was a good concentration for preserving the hookworm eggs. The second round fine tuned study indicated the optimal salt concentration was 36%. After three years,the preserved egg could be hatched and split into four;the egg shell was intact;the lacuna between egg shell and egg was a little larger;the egg stopped development and had no distortion. Conclusion Hookworm eggs could be preserved with salt and still maintained their variability for long time.
引文
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[11]兰炜明,谢曙英,王沁,等.日本血吸虫活虫卵的保存[J].中国血吸虫病防治杂志,2015,27(5):523-528.Lan WM,Xie SY,Wang Q,et al.Preservation of live eggs of Schistosoma japonicum[J].Chin J Schisto Control,2015,27(5):523-528.
[2]陈颖丹,臧伟.我国土源性线虫病监测现状及今后监测工作重点[J].中国血吸虫病防治杂志,2015,27(2):111-114.Chen YD,Zang W.Current situation of soil transmitted nematodiasis monitoring in China andworking keys in future[J].Chin J Schisto Control,2015,27(2):111-114.
[3]卢作超,胡缨,刘晓泉,等.南宁12 313例医院就诊者肠道寄生虫感染状况分析[J].热带医学杂志,2015,15(5):677-680.Lu ZC,Hu Y,Liu XQ,et al.Analysis intestinal parasitic infection status of 12 313 cases of hospital patients in Nanning city,Guangxi[J].J Trop Med,2015,15(5):677-680.
[4]周晓农.寄生虫病检测技术[M].第1版.北京:人民卫生出版社,2011.Zhou XN.Diagnostic techniques of parasitic diseases[M].1th ed.Beijing:People′s Medical Publishing House,2011.
[5]缪峰,刘永春,赵长磊.现场调查寄生虫标本的收集、固定和保存方法[J].中国寄生虫学与寄生虫病杂志,2002,20(6):379.Miao F,Liu YL,Zhao CL.Collection fixation and preservation technique of parasite specimens for field survey[J].Chin J Parasitol Parasit Dis,2002,20(6):379.
[6]常正山.寄生虫标本的采集和保存[J].中国寄生虫学与寄生虫病杂志,2006,24(增刊):s76-s81.Chang ZS.Collection of Parasite Samples and Preservation of Specimens[J].Chin J Parasitol Parasit Dis,2006,24(Suppl):s76-s81.
[7]李朝品.人体寄生虫学实验研究技术[M].第1版.北京:人民卫生出版社,2008.Li CP.Experimental techniques in human parasitology[M].1th ed.Beijing:People's Medical Publishing House,2008.
[8]李辉.甘油蛋白等渗混封液封制钩虫卵永久标本初探[J].广西中医学院学报,2000,17(1):82.Li H.Study on make the permanent sample of hookworm egg by Glycerin and other osmotic sealant[J].Journal of Guangxi Traditional Chinese Medical University,2000,17(1):82.
[9]谢庆敢,陆运龙,杜传,等.酒精溶液保存肠道蠕虫卵形态效果观察[J].中国寄生虫病防治杂志,2001,14(3):X.Xie QG,Lu YL,DU Z,et al.Observe the morphological effect of helminth eggs preserved by alcohol solution[J].Chin J Parasit Dis Con,2001,14(3):X.
[10]朱明磊,郭鄂平.制作寄生虫虫卵长时间保存标本的研究[J].医学动物仿制,2010,26(9):873.Zhu ML,Guo EP.Study on preserving specimens of parasite eggs for a long time[J].J Med Pest Control,2010,26(9):873.
[11]兰炜明,谢曙英,王沁,等.日本血吸虫活虫卵的保存[J].中国血吸虫病防治杂志,2015,27(5):523-528.Lan WM,Xie SY,Wang Q,et al.Preservation of live eggs of Schistosoma japonicum[J].Chin J Schisto Control,2015,27(5):523-528.