非洲新稻与籼稻杂交后代育性及主要农艺性状的观察鉴定
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摘要
【目的】探明非洲新稻与籼稻育性及主要农艺性状表现,为在水稻育种中利用非洲新稻资源提供理论依据和材料基础。【方法】利用非洲新稻Z3与籼稻龙特浦B杂交获得F1群体,再自交获得F2群体,在水稻成熟期调查亲本和各群体的结实率及主要农艺性状。【结果】Z3与龙特浦B杂交F1的自交结实率为70%,F2群体的自交结实率为24%~80%;F2群体中各性状都存在变异,可从中筛选到综合农艺性状优良的单株。相关分析结果表明,杂交F2群体单株产量与株实粒数、株穗数、结实率和穗长之间存在极显著正相关,与株高之间存在显著正相关。【结论】非洲新稻Z3与籼稻龙特浦B杂交后代之间不存在自交不育问题,可在水稻育种中直接利用。
【Objective】The present research was conducted to evaluate the fertility and important agronomic traits in the offspring derived from cross between new rice for Africa (NERICA) and Indica rice (Oryza sativa L.), in order to provide theoretical references and materials for NERICA utilization in rice breeding. 【Method】NERICA cultivar Z3 was crossed with Indica rice cultivar Longtepu B to produce F1population and selfing F2generation. The fertility and important agronomic traits of each generation were evaluated at the maturing stage of rice. 【Result】The seed setting rate of F1generation was 70%, and the seed setting rate of F2population ranged from 24% to 80%. Variation was found in F2generation for all traits,and individual plant with excellent agronomic comprehensive traits could be screened out from F2population. Grain yield per plant exhibited extremely significantly positive correlation with filled grains per plant, panicles per plant, seed setting rate and panicle length, as well as significant positive correlation with plant height. 【Conclusion】There was no self-sterility in filial generations between NERICA and Indica rice. It is feasible to directly apply NERICA in rice breeding.
【Objective】The present research was conducted to evaluate the fertility and important agronomic traits in the offspring derived from cross between new rice for Africa (NERICA) and Indica rice (Oryza sativa L.), in order to provide theoretical references and materials for NERICA utilization in rice breeding. 【Method】NERICA cultivar Z3 was crossed with Indica rice cultivar Longtepu B to produce F1population and selfing F2generation. The fertility and important agronomic traits of each generation were evaluated at the maturing stage of rice. 【Result】The seed setting rate of F1generation was 70%, and the seed setting rate of F2population ranged from 24% to 80%. Variation was found in F2generation for all traits,and individual plant with excellent agronomic comprehensive traits could be screened out from F2population. Grain yield per plant exhibited extremely significantly positive correlation with filled grains per plant, panicles per plant, seed setting rate and panicle length, as well as significant positive correlation with plant height. 【Conclusion】There was no self-sterility in filial generations between NERICA and Indica rice. It is feasible to directly apply NERICA in rice breeding.
引文
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徐鹏,陶大云,胡凤益,周家武,李静,邓先能.2005.栽培稻种间杂交改良云南粳稻品种研究[J].中国水稻科学,19(1):41-46.Xu P,Tao D Y,Hu F Y,Zhou J W,Li J,Deng X N.2005.Interspecific hybridization of cultivated rice for breedingjaponica rice in Yunnan Province[J].Chinese Journal ofRice Science,19(1):41-46.
Africa Rice Center(WARDA)/FAO/SAA.2008.NERICA:theNew Rice for Africa-a Compendium[C]//Somado E A,Guei R G,Keya S O.Cotonou,Benin:Africa Rice Center(WARDA);Rome,Italy:FAO;Tokyo,Japan:SasakawaAfrica Association:62-193.
Ghesquiere A,Sequier J,Second G,Lorieux M.1997.Firststeps towards a rational use of African rice,Oryza glaberri-ma,in rice breeding through a‘contigline’concep[tJ].E-uphytica,96:31-39.
Khush G S.1997.Origin,dispersal,cultivation and variation ofrice[J].Plant Molecular Biology,35:25-34.
Lorieux M,Ndjiondjop M N,Ghesquiere A.2000.A first in-terspecific Oryza sativa×Oryza glaberrima microsatellite-based genetic linkage map[J].Theoretical and Applied Ge-netics,100:593-601.
Li Z M,Zheng X M,Ge S.2011.Genetic diversity and domes-tication history of African rice(Oryza glaberrima)as inferredfrom multiple gene sequences[J].Theoretical and AppliedGenetics,123:21-31.
Monty J.2004.NERICA fighting Africa’s war against povertyand hunger[C].Des Moines,Iowa,USA:InternationalYear of Rice&World Food Prize Celebration,October14-15,2004.
Pental D,Barnes S R.1985.Interrelationship of cultivated ricesOryza sativa and O.glaberrima with wild O.perennis com-plex[J].Theoretical and Applied Genetics,70:185-191.
Reversat G,Destombes D.1995.Resistance to Heteroderasac-chari in rice[J].Nematologica,41:333-334.
Second G.1982.Origin of the genic diversity of cultivated rice(Oryza spp.):Study of the polymorphism scored at40isozyme loc[iJ].Japanese Journal of Genetics,57:25-57.
Silue D,Notteghem J.1991.Resistance of99Oryza glaberrimaSteud varieties to blas[tJ].International Rice Research Notes,16:13-14.
栾鸭红,周家武,胡凤益,李静,徐鹏,邓先能,谭学林,陶大云.2009.栽培稻种间育性S1基因桥梁亲本培育及分子验证研究[J].西南大学学报:自然科学版,31(12):21-29.Luan Y H,Zhou J W,Hu F Y,Li J,Xu P,Deng X N,Tan XL,Tao D Y.2009.Study on development of S1near iso-genic line and molecular confirmation in rice(Oryza sativaL.)[J].Journal of Southwest University:Natural ScienceEdition,31(12):21-29.
徐鹏,陶大云,胡凤益,周家武,李静,邓先能.2005.栽培稻种间杂交改良云南粳稻品种研究[J].中国水稻科学,19(1):41-46.Xu P,Tao D Y,Hu F Y,Zhou J W,Li J,Deng X N.2005.Interspecific hybridization of cultivated rice for breedingjaponica rice in Yunnan Province[J].Chinese Journal ofRice Science,19(1):41-46.
Africa Rice Center(WARDA)/FAO/SAA.2008.NERICA:theNew Rice for Africa-a Compendium[C]//Somado E A,Guei R G,Keya S O.Cotonou,Benin:Africa Rice Center(WARDA);Rome,Italy:FAO;Tokyo,Japan:SasakawaAfrica Association:62-193.
Ghesquiere A,Sequier J,Second G,Lorieux M.1997.Firststeps towards a rational use of African rice,Oryza glaberri-ma,in rice breeding through a‘contigline’concep[tJ].E-uphytica,96:31-39.
Khush G S.1997.Origin,dispersal,cultivation and variation ofrice[J].Plant Molecular Biology,35:25-34.
Lorieux M,Ndjiondjop M N,Ghesquiere A.2000.A first in-terspecific Oryza sativa×Oryza glaberrima microsatellite-based genetic linkage map[J].Theoretical and Applied Ge-netics,100:593-601.
Li Z M,Zheng X M,Ge S.2011.Genetic diversity and domes-tication history of African rice(Oryza glaberrima)as inferredfrom multiple gene sequences[J].Theoretical and AppliedGenetics,123:21-31.
Monty J.2004.NERICA fighting Africa’s war against povertyand hunger[C].Des Moines,Iowa,USA:InternationalYear of Rice&World Food Prize Celebration,October14-15,2004.
Pental D,Barnes S R.1985.Interrelationship of cultivated ricesOryza sativa and O.glaberrima with wild O.perennis com-plex[J].Theoretical and Applied Genetics,70:185-191.
Reversat G,Destombes D.1995.Resistance to Heteroderasac-chari in rice[J].Nematologica,41:333-334.
Second G.1982.Origin of the genic diversity of cultivated rice(Oryza spp.):Study of the polymorphism scored at40isozyme loc[iJ].Japanese Journal of Genetics,57:25-57.
Silue D,Notteghem J.1991.Resistance of99Oryza glaberrimaSteud varieties to blas[tJ].International Rice Research Notes,16:13-14.