健脾益智胶囊对大脑中动脉栓塞大鼠海马Hes1表达的影响
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摘要
目的:探讨健脾益智胶囊对大脑中动脉栓塞(MCAO)大鼠转录因子发状分裂相关增强子1(hairy and enhancer of s Plit,Hes1)表达的影响。方法:建立MCAO大鼠模型,随机分为正常对照组、模型对照组、假手术组、西药组(予尼莫地平)和中药组(予健脾益智胶囊)。采用Western Blotting(蛋白免疫印迹)、RT-PCR(实时荧光定量)方法于灌胃后7 d、14 d、28 d观察各组大鼠海马Hes1的蛋白及mRNA的表达情况。结果:MCAO大鼠造模后Hes1m RNA的含量和Hes1蛋白含量均明显降低,与正常对照组比较有显著性差异(P<0.05);经健脾益智胶囊和尼莫地平干预后,不同时间点中药组和西药组的Hes1蛋白和mRNA含量均明显高于模型对照组(P<0.05),其中28 d时中药组的Hes1蛋白和mRNA含量均高于西药组(P<0.05)。结论:健脾益智胶囊能够提高MCAO大鼠海马Hes1蛋白及基因的表达,提示健脾益智胶囊可能通过调控Hes1在大鼠海马中的含量以促进缺血后神经干细胞的增殖分化。
Objective:To investigate the effects of Jian Pi Yizhi capsule on expressions of hairy and enhancer of s Plit(Hes1)in hippocampus of rats with middle cerebral artery occlusion(MCAO). Methods:The model of MCAO rats was established and ra ndomly divided into normal control group,model control group,sham operation group,western medicine group(nimodipine) and Chinese medicine(CM) group(Jian Pi Yizhi capsule). The protein and mRNA expression of Hes1 in hippocampus of rats in each group were observed at 7 d,14 d and 28 d after intragastric administration by Western Blotting and RT-PCR. Results:The Hes1 mRNA content and Hes1 protein content in MCAO rats were significantly lower than that of the normal control group(P <0.05). After the intervention of Jianpi Yizhi capsule and nimodipine,the protein and mRNA contents of Hes1 in the CM and western medicine groups at different time points were significantly higher than those in the model control group(P <0.05). And the protein and mRNA contents of Hes1 in Chinese medicine group at 28 d was higher than that in the western medicine group(P <0.05).Conclusion:JianPi Yizhi capsule can improve the expression of Hes1 protein and gene in hippocampus of MCAO rats.It is suggested that Jian Pi Yizhi capsule may promote the proliferation and differentiation of neural stem cells after ischemia by regulating the content of Hes1 in rat hippocampus.
Objective:To investigate the effects of Jian Pi Yizhi capsule on expressions of hairy and enhancer of s Plit(Hes1)in hippocampus of rats with middle cerebral artery occlusion(MCAO). Methods:The model of MCAO rats was established and ra ndomly divided into normal control group,model control group,sham operation group,western medicine group(nimodipine) and Chinese medicine(CM) group(Jian Pi Yizhi capsule). The protein and mRNA expression of Hes1 in hippocampus of rats in each group were observed at 7 d,14 d and 28 d after intragastric administration by Western Blotting and RT-PCR. Results:The Hes1 mRNA content and Hes1 protein content in MCAO rats were significantly lower than that of the normal control group(P <0.05). After the intervention of Jianpi Yizhi capsule and nimodipine,the protein and mRNA contents of Hes1 in the CM and western medicine groups at different time points were significantly higher than those in the model control group(P <0.05). And the protein and mRNA contents of Hes1 in Chinese medicine group at 28 d was higher than that in the western medicine group(P <0.05).Conclusion:JianPi Yizhi capsule can improve the expression of Hes1 protein and gene in hippocampus of MCAO rats.It is suggested that Jian Pi Yizhi capsule may promote the proliferation and differentiation of neural stem cells after ischemia by regulating the content of Hes1 in rat hippocampus.
引文
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[2]Wall D S,Mears A J,Mc Neill B,et al.Progenitor cell proliferation in the retina is dependent on Notch-indepen dent sonic hedgehog/Hesl activity[J].J Cell Biol,2009,184(1):101-112.
[3]Longa E Z,Weinstein P R,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
[4]Bederson J B,Pitts L H,Tsuji M,et al.Rat middle cerebral artery occlusion:evaluation of the model and de velopment of a neurologic examination[J].Stroke,1986,17(3):472-476.
[5]Li Y,Hiroi Y,Liao J K.Notch signaling as an important mediator of cardiac repair and regeneration after myocardial infarction[J].Trends in Cardiovascular Medicine,2010,20(7):228.
[6]刘柏炎,蔡光先.补阳还五汤对大鼠局灶性脑缺血后血管内皮生长因子及其受体F1k1的影响[J].中草药,2007,38(3):394-397.
[7]田兆华,刘柏炎.补阳还五汤对局灶性脑缺血大鼠血管新生的影响[J].中国动脉硬化杂志,2010,18(3):193-198.
[8]张均克,邱幸凡,张六通.论祛瘀生新促血管新生的理论基础[J].湖北中医学院学报,2010,12(5):47-48.
[9]冯珂,纪立金.健脾益智胶囊对大脑中动脉栓塞大鼠海马神经干细胞增殖及Notch1,Jagged1表达的影响[J].中华中医药杂志,2013,28(4):921-924.
[10]谢越,李斌,林山,等.健脾益智胶囊对大脑中动脉栓塞大鼠脑组织自由基代谢的影响[J].广西中医药,2018,41(1):57-60.