洋葱霍尔德氏菌对玫瑰香葡萄采后灰霉病的抑制活性
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摘要
为研究生防菌洋葱霍尔德氏菌B-1的生防潜力,并为其生产性应用奠定基础,研究洋葱霍尔德氏菌B-1的生物量最大值的培养时间、浓度和发酵时间对玫瑰香葡萄采后病害的抑制效果。结果表明,在接种后24 h,拮抗菌B-1的抑菌效果最好,抑菌圈直径达2.81 cm。在液体培养基中,生防菌可有效抑制灰葡萄孢霉孢子萌发和芽管伸长,稀释5倍发酵液和发酵原液可完全抑制灰葡萄孢霉孢子萌发,稀释40倍发酵液孢子萌发率下降60.39%。在0,16℃和26℃温度条件下,拮抗菌均可显著降低葡萄采后自然腐烂率。菌株B-1不论是在离体条件还是在活体条件下,均有抑制灰葡萄孢霉的作用,能有效防治葡萄采后灰霉病的发生,是一株生防潜力较好的菌株。
To study the antagonistic Burkholderia bacteria onion Burkholderia contaminans B-1 biocontrol effect and lay the foundation for production applications. Culture time for maximum biomass, activity under different concentration and culture time inhibitory effect of Burkholderia contaminans B-1 against disease of postharvest grape investigated. Inhibition effect was studied using the methods of Confrontation culture, Oxford Cup and the spore germination against Botrytis cinerea. The results showed that after inoculation 24 h, the inhibitory activity of antagonistic bacteria B. contaminans reached highest with inhibition zone diameter of up to 2.81 cm. In the liquid medium, the bacteria can effectively suppress spore germination and germ tube elongation of Botrytis mold. Diluted 5-fold fermentation and fermentation liquid can be completely suppressed spore germination of B. cinerea. The pore germination rate of Botrytis mold decreased60.39% in diluted 40-fold broth. At 0, 16 ℃ and 26 ℃ the B. contaminans could significantly reduce the rate of natural decay after the grape harvest. B. contaminans strain either in vitro or in vivo can effectively prevent the occurrence of gray mold after grape harvest, and is a strain with good potential biocontrol.
To study the antagonistic Burkholderia bacteria onion Burkholderia contaminans B-1 biocontrol effect and lay the foundation for production applications. Culture time for maximum biomass, activity under different concentration and culture time inhibitory effect of Burkholderia contaminans B-1 against disease of postharvest grape investigated. Inhibition effect was studied using the methods of Confrontation culture, Oxford Cup and the spore germination against Botrytis cinerea. The results showed that after inoculation 24 h, the inhibitory activity of antagonistic bacteria B. contaminans reached highest with inhibition zone diameter of up to 2.81 cm. In the liquid medium, the bacteria can effectively suppress spore germination and germ tube elongation of Botrytis mold. Diluted 5-fold fermentation and fermentation liquid can be completely suppressed spore germination of B. cinerea. The pore germination rate of Botrytis mold decreased60.39% in diluted 40-fold broth. At 0, 16 ℃ and 26 ℃ the B. contaminans could significantly reduce the rate of natural decay after the grape harvest. B. contaminans strain either in vitro or in vivo can effectively prevent the occurrence of gray mold after grape harvest, and is a strain with good potential biocontrol.
引文
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[11]侯毅平,章四平,王建新,等.枯草芽孢杆菌NJ-18对油菜菌核病的防治效果及定殖动态[J].植物病理学报,2013,43(4):411-417.
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[13]施俊凤,薛梦林,张晓宇,等.梅奇酵母XY201对冬枣采后病原真菌的抑制效果[J].中国生物防治,2010,26(3):287-292.
[14]汪茜,胡春锦,柯仿钢,等.生防菌株1404的鉴定及其对采后柑橘炭疽病的防治效果[J].微生物学报,2010,50(9):1208-1217.
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[21]于昕,邓婷婷,雷高鹏,等.草莓灰霉孢子萌发条件研究[J].应用与环境生物学报,2006,12(1):9-12.
[22]王彩霞,张清明,李桂舫,等.苹果树腐烂病拮抗细菌菌株BJI的鉴定及其抑菌作用[J].植物保护学报,2012,39(5):431-437.
[23]权春善,郑维,曹治明,等.洋葱伯克霍尔德菌CF-66抗菌物质的分离纯化及性质的研究[J].微生物学报,2005,45(5):707-710.
[24]江木兰,赵瑞,胡小加,等.油菜内生生防菌BY-2在油菜体内的定殖与对油菜菌核病的防治作用[J].植物病理学报,2007,37(2):192-196.
[25]陈士云,杨宝玉,高梅影,等.一株抑制油菜核盘菌菌核形成的解淀粉芽孢杆菌[J].应用与环境生物学报,2005,11(3):373-376.
[2]陈宇飞,文景芝,李立军.葡萄灰霉病研究进展[J].东北农业大学学报,2006,37(5):693-699.
[3]孔秋莲,修德仁,胡文玉,等.葡萄贮藏中SO2伤害与膜脂过氧化的关系[J].果树学报,2008,25(3):322-326.
[4]REIST M,JENN P,HALLIVELL B.Sulphite enhances peroxynitrite-dependent-antiproteinase inactivation.A mechanism of lung injury sulphur dioxide?[J].Febs letters,1998,423(2):231-234.
[5]吴忠红,马晓芬,张婷,等.二氧化硫保鲜纸对无核白葡萄果实采后品质的影响新疆农业科学[J].2014,51(8):1512-1518.
[6]鹿连明,杜丹超,程保平,等.海洋放线菌A3202的分离鉴定及其对柑橘采后病害的防效[J].植物保护学报,2014,6(41):327-334.
[7]李强,张红印,杨其亚,等.一株生防酵母菌的筛选鉴定及对葡萄采后病害的生防效果[J].食品工业科技,2014,35(14):183-185.
[8]ZAHAVI T,COHEN L,WEISS B,et al.Biological control of Botrytis,Aspergillus and Rhizopus rots on table and wine grapes in Israel[J].Plant Dis,2000,20(2):115-124.
[9]方中达.植病研究方法[M].北京:中国农业出版社,1998:182-183.
[10]NIEMANN H,LOSEKANN T,BEER D D,et al.Novel microbial communities of the Haakon Mosby mud volcano and their role as a methane sink[J].Nature,2006,443(5227):854-858.
[11]侯毅平,章四平,王建新,等.枯草芽孢杆菌NJ-18对油菜菌核病的防治效果及定殖动态[J].植物病理学报,2013,43(4):411-417.
[12]王小兵,骆永明,刘五星,等.花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效[J].中国生物防治学报,2011,27(1):88-92.
[13]施俊凤,薛梦林,张晓宇,等.梅奇酵母XY201对冬枣采后病原真菌的抑制效果[J].中国生物防治,2010,26(3):287-292.
[14]汪茜,胡春锦,柯仿钢,等.生防菌株1404的鉴定及其对采后柑橘炭疽病的防治效果[J].微生物学报,2010,50(9):1208-1217.
[15]COENYE T,VANDAMME P,GOVAN J R W,et al.Taxonomy and identification of the Burkholderia cepacia complex[J].Journal of Clinical Microbiology,2001,39(10):3427-3436.
[16]COENYE T,VANDAMME P.Diversity and significance of Burkholderia species occupying diverse ecological niches[J].Environmental Microbiology,2003,5(9):719-729.
[17]VANLAERE E,BALDWIN A,GEVERS D,et al.Taxon K,a complex within the Burkholderia cepacia complex,comprises at least two novel species,Burkholderia contaminans sp.nov.and Burkholderia lata sp.nov.[J]International Journal of Systematic and Evolutionary Microbiology,2009,59(1):102-111.
[18]PARKE J L,GURIAN-SCHERMA D.Diversity of the Burkholderia cepacia complex and implications for risk assessment of biological control strains[J].Annual Review of Phytopathology,2001,39(9):225-258.
[19]范青,田世平,姜爱丽,等.采摘后果实病害生物防治拮抗菌的筛选和分离[J].中国环境科学,2001,21(4):313-316.
[20]谢关林,金扬秀,徐传雨,等.我国水稻纹枯病拮抗细菌种类研究[J].中国生物防治,2003,19(4):166-170.
[21]于昕,邓婷婷,雷高鹏,等.草莓灰霉孢子萌发条件研究[J].应用与环境生物学报,2006,12(1):9-12.
[22]王彩霞,张清明,李桂舫,等.苹果树腐烂病拮抗细菌菌株BJI的鉴定及其抑菌作用[J].植物保护学报,2012,39(5):431-437.
[23]权春善,郑维,曹治明,等.洋葱伯克霍尔德菌CF-66抗菌物质的分离纯化及性质的研究[J].微生物学报,2005,45(5):707-710.
[24]江木兰,赵瑞,胡小加,等.油菜内生生防菌BY-2在油菜体内的定殖与对油菜菌核病的防治作用[J].植物病理学报,2007,37(2):192-196.
[25]陈士云,杨宝玉,高梅影,等.一株抑制油菜核盘菌菌核形成的解淀粉芽孢杆菌[J].应用与环境生物学报,2005,11(3):373-376.