TNIP1基因沉默对系统性红斑狼疮患者B细胞上BCR、CD40基因表达的影响
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摘要
目的探讨TNIP1基因沉默对系统性红斑狼疮(SLE)患者B细胞上BCR和CD40基因表达的影响。方法收集2018年3月武汉大学医院接收的20例SLE患者和20例健康体检者的血液标本,以慢病毒介导TNIP1基因沉默为切入点,采用实时荧光定量PCR方法检测细胞中的TNIP1基因中mRNA的表达水平以及BCR、CD40的表达水平。结果 TNIP1基因在SLE患者中的表达量较高,干扰B细胞中TNIP1基因可导致B细胞中BCR和CD40表达下调。结论 TNIP1、BCR和CD40基因在B细胞中的表达具有一致的表达趋势,提示TNIP1、BCR、CD40有一定的调控关系。
Objective To explore the effect of TNIP1 gene silence on the expression of BCR and CD40 gene in B cells of patients with systemic lupus erythematosus(SLE). Methods Blood samples from 20 SLE patients and 20 healthy subjects received in Wuhan University Hospital during March 2018 were collected. Lentiviral-mediated TNIP1 gene silence was used as an entry point. Real-time quantitative PCR was used to detect mRNA expression level as well as expression levels of BCR and CD40 in TNIP1 gene in cells. Results The expression level of TNIP1 gene was higher in SLE patients, and interference with TNIP1 gene in B cells could lead to down-regulation of BCR and CD40 expression in B cells. Conclusion The expression of TNIP1, BCR and CD40 genes in B cells show a consistent trend, suggesting that TNIP1, BCR and CD40 have a certain regulatory relationship.
Objective To explore the effect of TNIP1 gene silence on the expression of BCR and CD40 gene in B cells of patients with systemic lupus erythematosus(SLE). Methods Blood samples from 20 SLE patients and 20 healthy subjects received in Wuhan University Hospital during March 2018 were collected. Lentiviral-mediated TNIP1 gene silence was used as an entry point. Real-time quantitative PCR was used to detect mRNA expression level as well as expression levels of BCR and CD40 in TNIP1 gene in cells. Results The expression level of TNIP1 gene was higher in SLE patients, and interference with TNIP1 gene in B cells could lead to down-regulation of BCR and CD40 expression in B cells. Conclusion The expression of TNIP1, BCR and CD40 genes in B cells show a consistent trend, suggesting that TNIP1, BCR and CD40 have a certain regulatory relationship.
引文
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[2]吴元胜,范瑞强,吴晓霞,等.系统性红斑狼疮临证研究现状评析[J].中医药学刊,2004,22(5):889-891.
[3]蒋季杰.现代红斑狼疮病学[M].北京:人民军医出版社,2005:110-111.
[4]朱琴芳,丁倩倩,俞钱,等.CD40-1C/T基因多态性与系统性红斑狼疮的相关性研究[J].现代检验医学杂志,2015,30(6):46-48.
[5]Yan YW,Fan J,Bai SL,et al.Zinc prevents abdominal aortic aneurysm formation by induction of A20-mediated suppression of NF-κB pathway[J].PLoS One,2016,11(2):e0148536.
[6]吴成将,袁秋然,莫益姣,等.CD40基因多态性及血清水平与系统性红斑狼疮发病机制的相关研究[J].中华皮肤科杂志,2016,49(1):12-16.
[7]弓娟琴.CD40/CD40配体的免疫调节与系统性红斑狼疮[J]国外医学:皮肤性病学册,2000,26(6):336-339.
[8]卫斐然.系统性红斑狼疮(SLE)与TNIP1基因相关性的Meta分析[J].中华疾病控制杂志,2015,19(11):1152-1157.
[9]Del Pino-Sedeno T,Trujillo-Martin MM,Ruiz-Irastorza G,et al.Effectiveness of non-pharmacological interventions for decreasing fatigue in adults with systemic lupus erythematosus:a systematic review[J].Arthritis Care Res,2015,11(6):125-131.
[10]陈荣威.TNIP1基因与SLE相关性研究进展[J].皮肤病与性病,2011,33(3):156-158.
[11]叶霜,陈小青.系统性红斑狼疮发病机制的研究现状和趋势[J].内科理论与实践,2008,3(3):162-167.
[12]董忠生,李素娟,佘红纯,等.河南汉族系统性红斑狼疮患者HS1基因EPEP插入多态性检测[J].郑州大学学报(医学版),2010,45(5):838-839.
[13]Gateva V,Sanding JK,Horn G,et al.A large-scale replication study identifies TNIP1,PRDM1,JAZF1,UHRF1BP1and IL10 as risk loci for systemic lupus erythematosus[J].Nat Genet,2009,41(11):1228-1233.
[14]邓迎苏.系统性红斑狼疮多系统损害与中医辨证关系[J].上海中医药大学学报,2000,14(4):27-28.
[15]Rothwarf DM,Karin M.The NF-kappa B activation pathway:a paradigm in information transfer from membrane to nucleus[J].Sci STKE,1999,(5):RE1.
[16]Meroni PL,Penatti AE.Epigenetics and systemic lupus erythema-tosus:unmet needs[J].Clin Rev Allergy Immunol,2015,50(3):367-376.
[17]蒋季杰.现代红斑狼疮病学[M].北京:人民军医出版社,2015:112.
[18]Spada R,Rojas JM,Barber DF.Recent findings on the role of natural killer cells in the pathogenesis of systemic lupus erythema-tosus[J].J Leukoc Biol,2015,98(4):479-487.
[19]G′Sell RT,Gaffney PM,Powell DW.A20-binding inhibitor of NF-κB activation 1 is a physiologic inhibitor of NF-κB:a molecular switch for inflammation and autoimmunity[J].Arthritis Rheumatol,2015,67(9):2292-2302.
[20]Wang F,Zhang X,Xia P,et al.Upregulation of tumor necrosis factor alpha-induced protein 3 interacting protein1 m RNA in psoriasis vulgaris[J].J Dtsch Dermatol Ges,2015,13(2):159-161.
[21]de Jesus GR,Mendoza-Pinto C,de Jesus NR,et al.Understanding and managing pregnancy in patients with lupus[J].Autoimmune Dis,2015,2015:943 490.