纳米金辅助介质阻挡放电离子化质谱分析法在兽药饲料快检中的应用
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摘要
介质阻挡放电离子化质谱分析法具有无需复杂样品前处理、分析速度快等优点,可实现样品原位、高效检测,成为质谱分析领域的热点技术。本研究提出了一种基于纳米金辅助纸基进样方式的介质阻挡放电离子源-质谱(DBDI-MS)联用技术,实现了氟甲喹、二甲氧苄啶、替硝唑、地美硝唑共4种兽药的快速检测。结果表明,在氦气流量为2.5 L/min、离子源温度为200℃等最优实验条件下,4种兽药检测信号强度均比无纳米金辅助时提高了8~31倍。4种兽药样品在各自的浓度范围内有较好的线性关系,相关系数(r)为0.952~0.996,且在饲料基质中的加标回收率为79.4%~96.5%。本方法能快速检测兽药饲料,具有灵敏度高、检测费用低、实用性好等优点,所得结果基本满足农业部限定标准要求,适合现场检测,在饲料质量控制方面具有较好的应用前景。
Dielectric barrier discharge ion source-mass spectrometry(DBDI-MS) can achieve in-situ and efficient detection of samples without tedious pretreatment,and has become a popular analytical method. To improve the detection sensitivity,a novel mass spectrometric method based on gold nanoparticles(Au NPs)-assisted paper-based injection mode and DBDI-MS technology was developed for the fast determination of four veterinary drugs. The detection signal strength was improved by 8-31 times compared to the veterinary drugs which were tested without Au NPs-assisted paper based injection. Under the optimal conditions, the experimental data of four veterinary drugs showed a good linear relationship between the signal and the concentration in the respective range,with correlation coefficient(r) of 0. 951-0. 996. Moreover,the recoveries were between 79.4% and 96.5% at three spiked levels. This method has many distinct advantages,such as low detected limitation,low cost and high efficiency,and is also very suitable for fast on-site detecting,showing great potential in feed quality control.
Dielectric barrier discharge ion source-mass spectrometry(DBDI-MS) can achieve in-situ and efficient detection of samples without tedious pretreatment,and has become a popular analytical method. To improve the detection sensitivity,a novel mass spectrometric method based on gold nanoparticles(Au NPs)-assisted paper-based injection mode and DBDI-MS technology was developed for the fast determination of four veterinary drugs. The detection signal strength was improved by 8-31 times compared to the veterinary drugs which were tested without Au NPs-assisted paper based injection. Under the optimal conditions, the experimental data of four veterinary drugs showed a good linear relationship between the signal and the concentration in the respective range,with correlation coefficient(r) of 0. 951-0. 996. Moreover,the recoveries were between 79.4% and 96.5% at three spiked levels. This method has many distinct advantages,such as low detected limitation,low cost and high efficiency,and is also very suitable for fast on-site detecting,showing great potential in feed quality control.
引文
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2 YANG Bing-Yi,ZHAI Hai-Yun,CHEN Gui-Xian,LUO Na,MO Jin-Yuan.Journal of Analytical Science,2009,25(6):657-660杨冰仪,翟海云,陈桂贤,罗娜,莫金垣.分析科学学报,2009,25(6):657-660
3 CHENG Lin-Li,ZHANG Su-Xia,SHEN Jian-Zhong,WANG Zhan-Hui,LI Yan-Shen,FENG Pei-Sheng.Chinese J.Anal.Chem.,2009,37(5):718-720程林丽,张素霞,沈建忠,王战辉,李彦伸,冯培生.分析化学,2009,37(5):718-720
4 LIN Xiao-Li,LI Ning,HUO Feng,DONG Yan-Feng,PENG Wei.Journal of Instrumental Analysis,2016,35(3):322-326林小莉,李宁,霍峰,董艳峰,彭玮.分析测试学报,2016,35(3):322-326
5 SUO De-Cheng,ZHAO Gen-Long,LI Lan,SU Xiao-Ou.Chinese J.Anal.Chem.,2010,38(7):1023-1026索德成,赵根龙,李兰,苏晓鸥.分析化学,2010,38(7):1023-1026
6 NING Lu-Sheng,XU Ming,GUO Cheng-An,ZHAO Peng,WEN Lu-Hong,ZHANG Xin-Rong.Chinese J.Anal.Chem.,2016,44(2):252-257宁录胜,徐明,郭成安,赵鹏,闻路红,张新荣.分析化学,2016,44(2):252-257
7 Na N,Zhang C,Zhao M,Zhang S,Yang C,Fang X.J.Mass Spectrom.,2007,42(8):1079-1085
8 Na N,Zhao M,Zhang S,Yang C,Zhang X.J.Mass Spectrom.,2007,18(10):1859-1862
9 Gong X,Xiong X,Peng Y,Yang C,Zhang S,Fang X.Talanta,2011,85(5):2458-2462
10 Huang G,Xu W,Visbal-Onufrak M A,Ouyang Z,Cooks R G.Analyst,2010,135(4):705
11 HONG Huan-Huan,ZHAO Peng,NING Lu-Sheng,WEN Lu-Hong,XU Tie-Feng.Chinese J.Anal.Chem.,2017,45(10):1529-1534洪欢欢,赵鹏,宁录胜,闻路红,徐铁峰.分析化学,2017,45(10):1529-1534
12 Glavan A C,Christodouleas D C,Mosadegh B,Yu H D,Smith B S,Lessing J.Anal.Chem.,2014,86(24):11999-12007
13 Martinez A W,Phillips S T,Whitesides G M,Carrilho E,Chem A.Anal.Chem.,2010,82(1):3-10
14 Scida K,Cunningham JC,Renault C,Richards I,Crooks R M.Anal.Chem.,2014,86(13):6501-6507
15 Li X,Ballerini D R,Shen W.Biomicrofluidics,2012,6(1):011301-13
16 Liu H,Crooks R M.J.Am.Chem.Soc.,2011,133(44):17564-17566
17 Kim J Y,Yeo M K.Mol.Cell.Toxicol.,2016,12(1):101-109
18 Bai Y,Feng F,Zhao L,Wang C,Wang H,Tian M.Biosens.Bioelectron.,2013,47(47C):265-270
19 WANG Hong-Ya,YIN Bin-Cheng,YE Bang-Ce.Chinese J.Anal.Chem.,2017,45(12):2018-2025王红亚,尹斌成,叶邦策.分析化学,2017,45(12):2018-2025
20 Darbha G K,Ray A,Ray P C.ACS Nano,2007,1(3):208-214
21 Yun C S,Javier A,Jennings T.J.Am.Chem.Soc.,2005,127(9):3115-3119
22 Gao M X,Zou H Y,Li Y F.Anal.Chem.,2017,89(3):1808-1814
23 Cody R B,Laramee J A,Nilles J M,Durst H D.Anal.Chem.,2005,77(8):2297-2302
24 Ministey of Agriculture.No.2349,Determination of Sulfonamides and Quinolones in Feeds-Liquid Chromatography Tandem Mass Spectrometry.Bulletion of Ministry of Agriculture of the People's Republic of China饲料中磺胺类和喹诺酮类药物的测定液相色谱—串联质谱法.中华人民共和国国家标准.农业部2349号公告-5-2015和-6-2015