桑叶褐斑病病原菌的生物学特性研究
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摘要
通过对桑叶褐斑病病原菌在不同种类培养基培养、不同氮源与碳源、不同温度等6种条件下培养6天后,测定菌落直径,观察菌丝颜色和菌落形态,分析了桑褐斑病病原菌的生物学特性。结果表明,在室内培养条件下,不同的营养成分、pH值、温度、光照都对菌丝的生长有影响。病原菌菌丝生长的最适宜培养基是燕麦片培养基,适宜生长温度范围是20~30℃,最适生长温度为25℃,光照对菌丝生长无显著影响,最适pH值为9~10,最适碳源为α-乳糖,最适氮源为尿素,菌丝在55℃水浴10 min后则不能生长。
Through cultivating pathogenic fungi of mulberry brown rot disease under 6 conditions such as different media,temperature,carbon source and nitrogen source after 6 days,research on the biological characteristics of pathogenic fungus causing brown spot on mulberry leaves was conducted. The results showed that nutrition,temperature and pH had significant influence on mycelial growth. It was found that the best medium for mycelial growth was oatmeal medium,the proper temperature was 20 ~ 30℃,the optimal temperature for mycelial growth was 25℃,the light had no obvious promotion effect on mycelial growth. It was also found that the optimal pH was9 to 10,the best carbon source and nitrogen source for mycelial grow are α-Galoctaose and Carbamide,and the mycelial could not survive above 55℃ more than 10 min.
Through cultivating pathogenic fungi of mulberry brown rot disease under 6 conditions such as different media,temperature,carbon source and nitrogen source after 6 days,research on the biological characteristics of pathogenic fungus causing brown spot on mulberry leaves was conducted. The results showed that nutrition,temperature and pH had significant influence on mycelial growth. It was found that the best medium for mycelial growth was oatmeal medium,the proper temperature was 20 ~ 30℃,the optimal temperature for mycelial growth was 25℃,the light had no obvious promotion effect on mycelial growth. It was also found that the optimal pH was9 to 10,the best carbon source and nitrogen source for mycelial grow are α-Galoctaose and Carbamide,and the mycelial could not survive above 55℃ more than 10 min.
引文
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[2]堵鹤鸣,方瑾芬.桑褐斑病菌生物学特性研究[J].蚕业科学,1996,22(4):214-218.
[3]Burgess L W,Summerell B A,Bullock A,et al.Laboratory Manual for Fusarium Research[M].3rd ed.Sydney:The University of Sydney,1994.
[4]孙俊,刘志恒,黄欣阳,等.辣椒褐斑病菌生物学特性研究[J].植物保护,2009,35(5):109-113.
[5]方中达.植病研究方法[M].北京:中国农业出版社,1996.
[6]黄桂辉.桑褐斑病的防治[J].植物医生,1999(6):15.
[7]王泽林.桑树褐斑病的药剂防治效果初报[J].四川蚕业,1999,27(1):25-26.
[8]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[9]管斌,徐超,张红岩,等.红叶石楠叶斑病病原菌分离鉴定及致病性测定研究[J].西部林业科学,2013,42(2):56-61.