外源水杨酸和脱落酸复合处理提高杜鹃花叶片耐热性
|
摘要
以生长一致2年生的"胭脂蜜"盆栽杜鹃花为试材,以喷施蒸馏水为对照(CK),以水杨酸(SA)、脱落酸(ABA)及SA+ABA复合处理喷施2次叶片为处理,研究了SA和ABA处理对39℃/29℃高温胁迫下叶片抗氧化和耐热性的影响,以期通过喷施SA和ABA来提高杜鹃花耐热能力。结果表明:与对照相比,经SA、ABA和SA+ABA复合处理均可提高叶片可溶性总糖、脯氨酸含量、相对含水量和叶绿素含量,降低叶片的相对电导率;促进叶片超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性增加,降低叶片H_2O_2和细胞膜脂过氧化产物丙二醛(MDA)含量。但以SA+ABA复合处理对提高杜鹃花的耐热性效果最好,ABA次之,然后是SA。因此,SA+ABA复合处理更能促进渗透物质合成,提高抗氧化能力和细胞完整性,从而提高对高温胁迫抗性。
To explore the effects of exogenous salicylic acid(SA)and abscisic acid(ABA)treatment on heat resistance of Rhododendron hybridum.2-year-old Rhododendron hybridum ‘Yanzhimi'were sprayed with 0(CK),10.0μmol·L~(-1) SA,30.0μmol·L~(-1) ABA and 10.0 μmol·L~(-1) SA+30.0μmol·L~(-1) ABA solution on the leaves and roots for two days,respectively.And then treated with heat stress(39 ℃/29 ℃,day/night)for 8 days by using plant growth chamber.The total chlorophyll content,osmotic regulation substances,membrane lipid peroxidation,and antioxidant enzymes activity of leaves were measured after different treatment times.The results showed that,compared with the control,treated with SA,ABA and SA+ ABA could markedly increased total soluble sugar,proline,relative water and chlorophyll contents in leaves,and decreased relative electrical conductivity,malondialdehyde(MDA)and H_2O_2 content in leaves.Meanwhile,activities of SOD and CAT in leaves were improved.However,compared with the control,treated SA coupled with ABA had the best effect improving heat resistance for Rhododendron hybridum,and the next were treated with ABA and SA.These results showed that treated with SA+ABA could improve osmotic regulation substances content,inhibite water loss,chlorophyll degradation and membrane oxidation,enhance the resistance to high temperature stress and alleviate the negative injuries.
To explore the effects of exogenous salicylic acid(SA)and abscisic acid(ABA)treatment on heat resistance of Rhododendron hybridum.2-year-old Rhododendron hybridum ‘Yanzhimi'were sprayed with 0(CK),10.0μmol·L~(-1) SA,30.0μmol·L~(-1) ABA and 10.0 μmol·L~(-1) SA+30.0μmol·L~(-1) ABA solution on the leaves and roots for two days,respectively.And then treated with heat stress(39 ℃/29 ℃,day/night)for 8 days by using plant growth chamber.The total chlorophyll content,osmotic regulation substances,membrane lipid peroxidation,and antioxidant enzymes activity of leaves were measured after different treatment times.The results showed that,compared with the control,treated with SA,ABA and SA+ ABA could markedly increased total soluble sugar,proline,relative water and chlorophyll contents in leaves,and decreased relative electrical conductivity,malondialdehyde(MDA)and H_2O_2 content in leaves.Meanwhile,activities of SOD and CAT in leaves were improved.However,compared with the control,treated SA coupled with ABA had the best effect improving heat resistance for Rhododendron hybridum,and the next were treated with ABA and SA.These results showed that treated with SA+ABA could improve osmotic regulation substances content,inhibite water loss,chlorophyll degradation and membrane oxidation,enhance the resistance to high temperature stress and alleviate the negative injuries.
引文
[1]何丽斯,苏家乐,刘晓青,等.不同杜鹃品种越夏能力的比较[J].江苏农业科学,2014,42(12):228-229.
[2]廖金,周泓,刘冰,等.夏季城市绿地中不同遮阳条件下杜鹃叶温变化及其生理响应[J].北方园艺,2013(7):74-77.
[3]庞新华,罗清,池昭锦.杜鹃耐热生理研究进展[J].北方园艺,2016(13):192-195.
[4]梁雯,赵冰,黄文梅.热锻炼对高温胁迫下2个杜鹃花品种耐热性的影响[J].东北林业大学学报,2017,45(9):24-30.
[5]吴伦忠,韩瑞红,莫亿伟,等.水杨酸提高水稻幼苗对镉胁迫的抗性[J].华北农学报,2008,23(增刊):135-139.
[6]王艳,李建龙,姜涛,等.SA,H2O2,6-BA预处理对沟叶结缕草耐寒性的影响[J].草业学报,2010,19(2):76-81.
[7]SHEN H F,ZHAO B,XU J J,et al.Effects of salicylic acid and calcium chloride on heat tolerance in Rhododendron‘Fen Zhenzhu’[J].Journal of the American Soceity Horticultural Science,2016,141(4):383-372.
[8]李畅,苏家乐,肖政,等.旱涝交替胁迫下杜鹃花叶片内源激素含量的变化[J].江苏农业科学,2016,44(7):233-235.
[9]耿兴敏,杨秋玉,郑福超,等.4种杜鹃幼苗高温胁迫下蛋白表达差异[J].分子植物育种,2016,14(6):1574-1581.
[10]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:110-165.
[11]罗萍,贺军军,姚艳丽,等.低温对不同耐寒性橡胶树叶片抗氧化能力的影响[J].西北植物学报,2014,34(2):311-317.
[12]郑小林,董任瑞.水稻热激反应的研究Ⅰ幼苗叶片的膜透性和游离脯氨酸含量的变化[J].湖南农业大学学报,1997,23(2):109-113.
[13]刘磊,陈立波,李志勇,等.晚秋温度对苜蓿地上部游离脯氨酸、可溶性糖和POD活性的影响[J].草业科学,2009,26(10):89-93.
[14]孙哲,范维娟,刘桂玲,等.干旱胁迫下外源ABA对甘薯苗期叶片光合特性及相关生理指标的影响[J].植物生理学报,2017,53(5):873-880.
[15]洪文君,王盼,刘强,等.毛棉杜鹃接菌苗对干旱胁迫的生理响应研究[J].西南农业学报,2016,29(4):805-809.
[16]申惠翡,赵冰,徐静静,等.外源水杨酸对西洋杜鹃耐热性的影响[J].东北林业大学学报,2016,44(7):44-45.
[17]NAYYAR H,BAINS T S,KUMAR S.Chilling stressed chickpea seedlings:Effect of cold acclimation,calcium and abscisic acid on cryoprotective solutes and oxidative damage[J].Environmental and Experimental Botany,2005,54:275-285.
[18]姜寒玉,王旺田,雷天翔,等.外源ABA和Ca2+对低温及低温恢复下赤霞珠幼苗蔗糖代谢的影响[J].干旱地区农业研究,2017,35(3):127-133.
[19]莫小锋,贲柳玲,邱莉维,等.外源ABA处理提高结缕草抗寒性试验[J].南方园艺,2014,25(3):6-10.
[20]隆春艳,古洪辉,汪正香,等.外源脱落酸对高温胁迫下菠菜光合与叶绿素荧光参数的影响[J].四川农业大学学报,2017,35(1):25-30.
[21]曹婧,兰海燕.植物激素脱落酸受体及其信号转导途径研究进展[J].生物技术通报,2014(4):22-28.
[22]SUN D,HUSSAIN H,YI Z,et al.Delivery of abscisic acid to plants using glutathione responsive mesoporous silica nanoparticles[J].Journal of Nanoscience and Nanotechnology,2017,17(1):1-11.
[23]MO Y,GONG D,LIANG G,et al.Enhanced preservation effects of sugar apple fruits by salicylic acid treatment during post-harvest storage[J].Journal of the Science of Food and Agriculture,2008,88(15):2693-2699.
[24]张宪政.水分亏缺下乙酰水杨酸清除小麦幼苗中O·2的研究[J].植物生理学通讯,2000,36(1):33-35.
[25]常云霞,徐克东,杨同文,等.外源水杨酸对野生龙葵幼苗干旱胁迫的缓解效应[J].干旱地区农业研究,2014,32(4):43-46.
[26]YI Z,HUSSAIN H,FENG C,et al.Functionalized mesoporous silica nanoparticles with redox-responsive short-chain gatekeepers for agrochemical delivery[J].ACS Appl Mater Interfaces,2015(7):9937-9946.
[27]肖强,王刚,衣艳君,等.外源脱落酸增强甘薯幼苗耐盐性的作用[J].植物营养与肥料学报,2016,22(1):201-208.
[2]廖金,周泓,刘冰,等.夏季城市绿地中不同遮阳条件下杜鹃叶温变化及其生理响应[J].北方园艺,2013(7):74-77.
[3]庞新华,罗清,池昭锦.杜鹃耐热生理研究进展[J].北方园艺,2016(13):192-195.
[4]梁雯,赵冰,黄文梅.热锻炼对高温胁迫下2个杜鹃花品种耐热性的影响[J].东北林业大学学报,2017,45(9):24-30.
[5]吴伦忠,韩瑞红,莫亿伟,等.水杨酸提高水稻幼苗对镉胁迫的抗性[J].华北农学报,2008,23(增刊):135-139.
[6]王艳,李建龙,姜涛,等.SA,H2O2,6-BA预处理对沟叶结缕草耐寒性的影响[J].草业学报,2010,19(2):76-81.
[7]SHEN H F,ZHAO B,XU J J,et al.Effects of salicylic acid and calcium chloride on heat tolerance in Rhododendron‘Fen Zhenzhu’[J].Journal of the American Soceity Horticultural Science,2016,141(4):383-372.
[8]李畅,苏家乐,肖政,等.旱涝交替胁迫下杜鹃花叶片内源激素含量的变化[J].江苏农业科学,2016,44(7):233-235.
[9]耿兴敏,杨秋玉,郑福超,等.4种杜鹃幼苗高温胁迫下蛋白表达差异[J].分子植物育种,2016,14(6):1574-1581.
[10]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:110-165.
[11]罗萍,贺军军,姚艳丽,等.低温对不同耐寒性橡胶树叶片抗氧化能力的影响[J].西北植物学报,2014,34(2):311-317.
[12]郑小林,董任瑞.水稻热激反应的研究Ⅰ幼苗叶片的膜透性和游离脯氨酸含量的变化[J].湖南农业大学学报,1997,23(2):109-113.
[13]刘磊,陈立波,李志勇,等.晚秋温度对苜蓿地上部游离脯氨酸、可溶性糖和POD活性的影响[J].草业科学,2009,26(10):89-93.
[14]孙哲,范维娟,刘桂玲,等.干旱胁迫下外源ABA对甘薯苗期叶片光合特性及相关生理指标的影响[J].植物生理学报,2017,53(5):873-880.
[15]洪文君,王盼,刘强,等.毛棉杜鹃接菌苗对干旱胁迫的生理响应研究[J].西南农业学报,2016,29(4):805-809.
[16]申惠翡,赵冰,徐静静,等.外源水杨酸对西洋杜鹃耐热性的影响[J].东北林业大学学报,2016,44(7):44-45.
[17]NAYYAR H,BAINS T S,KUMAR S.Chilling stressed chickpea seedlings:Effect of cold acclimation,calcium and abscisic acid on cryoprotective solutes and oxidative damage[J].Environmental and Experimental Botany,2005,54:275-285.
[18]姜寒玉,王旺田,雷天翔,等.外源ABA和Ca2+对低温及低温恢复下赤霞珠幼苗蔗糖代谢的影响[J].干旱地区农业研究,2017,35(3):127-133.
[19]莫小锋,贲柳玲,邱莉维,等.外源ABA处理提高结缕草抗寒性试验[J].南方园艺,2014,25(3):6-10.
[20]隆春艳,古洪辉,汪正香,等.外源脱落酸对高温胁迫下菠菜光合与叶绿素荧光参数的影响[J].四川农业大学学报,2017,35(1):25-30.
[21]曹婧,兰海燕.植物激素脱落酸受体及其信号转导途径研究进展[J].生物技术通报,2014(4):22-28.
[22]SUN D,HUSSAIN H,YI Z,et al.Delivery of abscisic acid to plants using glutathione responsive mesoporous silica nanoparticles[J].Journal of Nanoscience and Nanotechnology,2017,17(1):1-11.
[23]MO Y,GONG D,LIANG G,et al.Enhanced preservation effects of sugar apple fruits by salicylic acid treatment during post-harvest storage[J].Journal of the Science of Food and Agriculture,2008,88(15):2693-2699.
[24]张宪政.水分亏缺下乙酰水杨酸清除小麦幼苗中O·2的研究[J].植物生理学通讯,2000,36(1):33-35.
[25]常云霞,徐克东,杨同文,等.外源水杨酸对野生龙葵幼苗干旱胁迫的缓解效应[J].干旱地区农业研究,2014,32(4):43-46.
[26]YI Z,HUSSAIN H,FENG C,et al.Functionalized mesoporous silica nanoparticles with redox-responsive short-chain gatekeepers for agrochemical delivery[J].ACS Appl Mater Interfaces,2015(7):9937-9946.
[27]肖强,王刚,衣艳君,等.外源脱落酸增强甘薯幼苗耐盐性的作用[J].植物营养与肥料学报,2016,22(1):201-208.