泻肝补肾汤对实验性前列腺增生大鼠前列腺重量与上皮细胞高度及组织细胞核增殖抗原表达影响
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摘要
目的:分析泻肝补肾汤对良性前列腺增生(BPH)大鼠前列腺重量与上皮细胞高度及组织细胞核增殖抗原表达影响。方法:选取由本院实验动物中心提供的SPF级Wistar雄性大鼠30只,随机数字表法将大鼠分成三组,观察组(10只)、模型组(10只)和正常组(10只),模型组与观察组大鼠制备BPH模型,成功造模后观察组大鼠每天灌胃1ml/100g泻肝补肾汤,模型组和正常组灌胃等剂量生理盐水,连续灌胃30天,末次给药后检测各组大鼠体重、前列腺湿重及前列腺指数状况,腺体上皮细胞高度在光镜下检测,PCNA表达采用免疫组化检测。结果:模型组大鼠前列腺重量及前列腺指数较正常组显著升高,观察组大鼠前列腺重量及前列腺指数较模型组显著降低,差异均有统计学意义(P<0. 05);模型组大鼠上皮细胞高度及PCNA阳性率较正常组显著升高,观察组大鼠上皮细胞高度及PCNA阳性率较模型组显著降低,差异均有统计学意义(P<0. 05)。结论:泻肝补肾汤可降低BPH大鼠前列腺重量与上皮细胞高度,调节组织内PCNA表达对前列腺增生起抑制作用。
Objective: To analyze the effect of Xiegan Bushen Decoction on prostate weight,epithelial cell height,and expression of proliferating cell nuclear antigen in tissue of benign prostatic hyperplasia( BPH) rats. Methods: Thirty male Wistar rats of SPF grade were provided by our laboratory animal center. The rats were divided into three groups by random number table method. The observation group( 10),the model group( 10) and the normal group,10 rats in the model group and the observation group were used to prepare the BPH model. After the successful modeling,the rats in the observation group were given 1 ml/100 g Xiebu Bushen Decoction on a daily basis,and the model group and the normal group were given an equal dose of normal saline by gavage for 30 days. After the last administration,the body weight,prostate wet weight,and prostate index were measured. The height of glandular epithelial cells was detected under light microscope. The expression of PCNA was detected by immunohistochemistry. Results: Prostate weight and prostate index were significantly higher in the model group than in the normal group. The prostate weight and prostate index were significantly lower in the observation group than in the model group( P < 0.05),the model group had rat epithelium. The height of cells and the positive rate of PCNA were significantly higher than those in the normal group. The epithelial cell height and PCNA positive rate in the observation group were significantly lower than those in the model group,and the differences were statistically significant( P < 0. 05). Conclusion: Xiegan Bushen Decoction can reduce the weight of prostate and the height of epithelial cells in BPH rats,and regulate the expression of PCNA in the tissues of rats with inhibition of benign prostatic hyperplasia.
Objective: To analyze the effect of Xiegan Bushen Decoction on prostate weight,epithelial cell height,and expression of proliferating cell nuclear antigen in tissue of benign prostatic hyperplasia( BPH) rats. Methods: Thirty male Wistar rats of SPF grade were provided by our laboratory animal center. The rats were divided into three groups by random number table method. The observation group( 10),the model group( 10) and the normal group,10 rats in the model group and the observation group were used to prepare the BPH model. After the successful modeling,the rats in the observation group were given 1 ml/100 g Xiebu Bushen Decoction on a daily basis,and the model group and the normal group were given an equal dose of normal saline by gavage for 30 days. After the last administration,the body weight,prostate wet weight,and prostate index were measured. The height of glandular epithelial cells was detected under light microscope. The expression of PCNA was detected by immunohistochemistry. Results: Prostate weight and prostate index were significantly higher in the model group than in the normal group. The prostate weight and prostate index were significantly lower in the observation group than in the model group( P < 0.05),the model group had rat epithelium. The height of cells and the positive rate of PCNA were significantly higher than those in the normal group. The epithelial cell height and PCNA positive rate in the observation group were significantly lower than those in the model group,and the differences were statistically significant( P < 0. 05). Conclusion: Xiegan Bushen Decoction can reduce the weight of prostate and the height of epithelial cells in BPH rats,and regulate the expression of PCNA in the tissues of rats with inhibition of benign prostatic hyperplasia.
引文
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[12]马丁,尚吉文,王靖宇,等.前列腺癌与良性前列腺增生组织中细胞凋亡与增殖因子表达的组织芯片检测[J].临床泌尿外科杂志,2018,5(3):201~205
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[16]刘兵,彭志锋.α1a肾上腺素受体拮抗剂西洛多辛对大鼠良性前列腺增生的影响[J].中国医师杂志,2016,18(2):199~207
[2]李峰,郑仿,闫家文,等.加味桂枝茯苓颗粒对大鼠前列腺增生组织血管内皮生长因子和碱性成纤维细胞生长因子表达水平的影响[J].中国全科医学,2016,9(6):693~697
[3]杨涛,吴柏霖,周辉,等.沙芭特在非细菌性前列腺炎症中的抗炎、抗纤维化及促进上皮细胞凋亡作用的研究[J].现代泌尿生殖肿瘤杂志,2017,9(4):221~226
[4] Wijerathne C U,Park H S,Jeong H Y,et al. Quisqualis indica improves benign prostatic hyperplasia by regulating prostate cell proliferation and apoptosis[J]. Biological Pharmaceutical Bulletin, 2017, 40(12):104~108
[5]王毅,邵继春,张蜀武.雄激素致去势大鼠前列腺增生的组织形态学研究[J].中华男科学杂志,2002,8(3):190~193
[6]杜丽芬,陈镜楼.黄酮哌酯对前列腺增生模型大鼠的保护作用机制研究[J].中国药房,2016,27(31):4357~4359
[7]马莉,曾慧红,饶瑜红,等.大黄酸对受损肠黏膜上皮增殖细胞核抗原及PARP降解产物表达的影响[J].解剖学杂志,2016,39(6):664~666
[8] Maryam S,Mohaddeseh K,Saeedeh S,et al. Antiproliferative and Antioxidant Effects of Withania coagulans Extract on Benign Prostatic Hyperplasia in Rats[J]. Nephro-urology Monthly,2016,8(1):63~66
[9]任行飞,吴春磊,余沁楠,等.良性前列腺增生合并前列腺炎症患者前列腺液IL-8、IL-6与血清前列腺特异抗原的相关性[J].南方医科大学学报,2016,36(1):135~139
[10]崔栋,尚永刚,韩广玮,等.大鼠前列腺上皮细胞体外培养及其屏障功能的初步研究[J].中华男科学杂志,2016,22(2):133~137
[11]李峰,郑仿,谢江平,等.加味桂枝茯苓颗粒对实验性良性前列腺增生大鼠VEGF/Bcl-2表达及前列腺超微结构的影响[J].中华中医药学刊,2017,11(8):1977~1980
[12]马丁,尚吉文,王靖宇,等.前列腺癌与良性前列腺增生组织中细胞凋亡与增殖因子表达的组织芯片检测[J].临床泌尿外科杂志,2018,5(3):201~205
[13] Jeon W Y,Kim O S,Seo C S,et al. Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats[J]. Bmc Complementary&Alternative Medicine,2017,17(1):384~388
[14]裴昕奇,吴开杰,陈兴发,等.前列腺特异性抗原异常的前列腺增生患者穿刺及术后病理特征分析[J].现代泌尿外科杂志,2017,22(9):678~681
[15]徐晓林,赵瑞宁,聂黎虹,等.丙酸睾丸酮诱导去势大鼠前列腺增生模型的建立[J].宁夏医科大学学报,2017,7(3):256~259
[16]刘兵,彭志锋.α1a肾上腺素受体拮抗剂西洛多辛对大鼠良性前列腺增生的影响[J].中国医师杂志,2016,18(2):199~207