4株鸡传染性贫血病毒的分离鉴定及其遗传进化分析
|
摘要
为了解广东地区鸡传染性贫血病毒(CAV)的流行和变异情况,本研究于2014年从广东省大型鸡场采集了12份可疑样品,经PCR鉴定、全基因组测序分析和动物回归试验,共分离得到4株CAV(GD-101、GD-102、GD-103和GD-104)。动物实验结果显示:4株CAV能够引起鸡严重的骨髓黄化和胸腺萎缩,具有典型的高致病性病毒株特征。遗传进化分析结果表明,4株分离株处于同一进化分支(II分支),GD-101和GD-102株与中国GD-B-12株进化关系最近,GD-103及GD-104株与中国SC-SM株进化关系最近,表明本研究所分离到的4株CAV均为高致病性的II分支病毒株,本研究为深入研究广东地区CAV的流行情况提供了参考依据。
To investigate the prevalence of chicken anemia virus(CAV) in Guangdong region, four CAVs(GD-101, GD-102,GD-103 and GD-104, respectively) were isolated and identified from infected chicken in different areas in 2014. The pathogenic tests indicated that all the isolates were able to cause aplastic anemia and atrophy of the lymphoid tissue. Viral genomes were sequenced, and phylogenetic analysis showed that all the four CAVs belong to the II cluster, GD-101 and GD-102 were closely identical to the GD-B-12, GD-103 and GD-104 were closely identical to the SC-SM strain. All these data showed that the current isolates were highly pathogenic CAV.
To investigate the prevalence of chicken anemia virus(CAV) in Guangdong region, four CAVs(GD-101, GD-102,GD-103 and GD-104, respectively) were isolated and identified from infected chicken in different areas in 2014. The pathogenic tests indicated that all the isolates were able to cause aplastic anemia and atrophy of the lymphoid tissue. Viral genomes were sequenced, and phylogenetic analysis showed that all the four CAVs belong to the II cluster, GD-101 and GD-102 were closely identical to the GD-B-12, GD-103 and GD-104 were closely identical to the SC-SM strain. All these data showed that the current isolates were highly pathogenic CAV.
引文
[1]Schat K A.Chicken anemia virus[J].Curr top microbiol immunol,2009,331:151-183.
[2]Yuasa N,Taniguchi T,Yoshida I.Isolation and some characteristics of an agent inducing anemia in chicks[J].Avian Dis,1979,23(2):336-385.
[3]Zhou Wen-ping,Shen Bing,Yang Bing,et al.Isolation and identification of chicken infectious anemia virus in China[J].Avian Dis,1997,41(2):361-364.
[4]Kim H R,Kwon Y K,Bae Y C,et al.Molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in South Korea[J].Poultry Science,2010,89(11):2426-2431.
[5]Brown H K,Browning G F,Scott P C,et al.Full-length infectious clone of a pathogenic Australian isolate of chicken anaemia virus[J].Aust Vet J,2000,78(9):637-640.
[6]Sauvage V,Cheval J,Foulongne V,et al.Identification of the first human gyrovirus,a virus related to chicken anemia virus[J].J Virol,2011,85(15):7948-7950.
[7]Phan T G,Li L,O'Ryan M G,et al.A third gyrovirus species in human faeces[J].J Gen Virol,2012,93(6):1356-1361.
[8]Chu D K,Poon L L,Chiu S S,et al.Characterization of a novel gyrovirus in human stool and chicken meat[J].J lin Virol,2012,55,209-213.
[9]Maggi F,Macera L,Focosi D,et al.Human gyrovirus DNA in human blood,Italy[J].Emerg Infect Dis,2012,18(6):956-959.
[10]Yamaguchi S,Imada T,Kaji N,et al.Identification of a genetic determinant of pathogenicity in chicken anaemia virus[J].J Gen Virol,2001,82:1233-1238.
[11]Renshaw R W,Soine C,Weinkle T,et al.A hypervariable region in VP1 of chicken infectious anemia virus mediates rate of spread and cell tropism in tissue culture[J].J Virol,1996,70(12):8872-8878.
[2]Yuasa N,Taniguchi T,Yoshida I.Isolation and some characteristics of an agent inducing anemia in chicks[J].Avian Dis,1979,23(2):336-385.
[3]Zhou Wen-ping,Shen Bing,Yang Bing,et al.Isolation and identification of chicken infectious anemia virus in China[J].Avian Dis,1997,41(2):361-364.
[4]Kim H R,Kwon Y K,Bae Y C,et al.Molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in South Korea[J].Poultry Science,2010,89(11):2426-2431.
[5]Brown H K,Browning G F,Scott P C,et al.Full-length infectious clone of a pathogenic Australian isolate of chicken anaemia virus[J].Aust Vet J,2000,78(9):637-640.
[6]Sauvage V,Cheval J,Foulongne V,et al.Identification of the first human gyrovirus,a virus related to chicken anemia virus[J].J Virol,2011,85(15):7948-7950.
[7]Phan T G,Li L,O'Ryan M G,et al.A third gyrovirus species in human faeces[J].J Gen Virol,2012,93(6):1356-1361.
[8]Chu D K,Poon L L,Chiu S S,et al.Characterization of a novel gyrovirus in human stool and chicken meat[J].J lin Virol,2012,55,209-213.
[9]Maggi F,Macera L,Focosi D,et al.Human gyrovirus DNA in human blood,Italy[J].Emerg Infect Dis,2012,18(6):956-959.
[10]Yamaguchi S,Imada T,Kaji N,et al.Identification of a genetic determinant of pathogenicity in chicken anaemia virus[J].J Gen Virol,2001,82:1233-1238.
[11]Renshaw R W,Soine C,Weinkle T,et al.A hypervariable region in VP1 of chicken infectious anemia virus mediates rate of spread and cell tropism in tissue culture[J].J Virol,1996,70(12):8872-8878.