鸡传染性贫血病毒广东分离株GD-1-12致病性研究
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摘要
从广东省某商品代肉鸡场12日龄生长迟缓的小鸡体内分离到1株鸡传染性贫血病毒(CAV),暂定名为GD-1-12株。分离病毒用MDCC-MSB1细胞进行培养,通过肌肉注射方式感染1日龄SPF鸡,于感染后第7天、第14天、第21天分别检测CAV对SPF鸡体质量、胸腺、脾脏、肝脏、法氏囊及胸腺和外周血液中CD3+T淋巴细胞指标的影响,同时观察鸡的临床症状。结果表明,GD-1-12分离株导致SPF鸡30%的死亡率。感染后第14天,胸腺极度萎缩,肝脏肿大,法氏囊萎缩,脾脏萎缩。感染后第7~14天之间,胸腺和外周血中CD3+T淋巴细胞呈下降趋势。结果表明1日龄SPF雏鸡在人工感染CAV后第7~14天是损伤上述器官的关键时期。
A field strain GD-1-12of chicken anemia virus(CAV)was isolated from a 12-day-old broiler flock with growth runting syndromes from Gongdong province.MDCC-MSB1cells were used to proliferate the virus of GD-1-12isolate.SPF chickens at 1day were challenged with the virus by intramuscular injection. Ratios of thymuses,spleens,livers,the bursas to body weight,and the CD3+T cells in thymuses and peripheral blood were analyzed after infection at 7,14,21-day.Observation of clinical symptoms at 7,14, 21-day after infections with CAV.Results indicated that the GD-1-12isolate caused high mortality about 30%,belonging to highly pathogenic.After infection at 14day,resulting in severe thymic atrophy,hepatomegaly,bursa atrophy,spleen atrophy.After challenging at 7-14day,the CD3+T cells in thymuses and peripheral blood were significantly decreased,it indicated that T lymphocytes were the target cells. The research further illustrated that after infection at 7-14day was the critical period of the immune function with the serious obstacle.
A field strain GD-1-12of chicken anemia virus(CAV)was isolated from a 12-day-old broiler flock with growth runting syndromes from Gongdong province.MDCC-MSB1cells were used to proliferate the virus of GD-1-12isolate.SPF chickens at 1day were challenged with the virus by intramuscular injection. Ratios of thymuses,spleens,livers,the bursas to body weight,and the CD3+T cells in thymuses and peripheral blood were analyzed after infection at 7,14,21-day.Observation of clinical symptoms at 7,14, 21-day after infections with CAV.Results indicated that the GD-1-12isolate caused high mortality about 30%,belonging to highly pathogenic.After infection at 14day,resulting in severe thymic atrophy,hepatomegaly,bursa atrophy,spleen atrophy.After challenging at 7-14day,the CD3+T cells in thymuses and peripheral blood were significantly decreased,it indicated that T lymphocytes were the target cells. The research further illustrated that after infection at 7-14day was the critical period of the immune function with the serious obstacle.
引文
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[15]刘忠贵,郑世民,徐彦波,等.鸡贫血因子病的免疫病理学变化[J].中国农业科学,1997,30(4):74-82.
[16]Cardona C,Lucio B,O′Connell P,et al.Humoral immune response to chicken infectious anemia virus in three strains of chickens in a closed flock[J].Avian Dis,2000,44(3):661-667.
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[19]Otaaki Y,Nunoya T,Tajima M,et al.Depression of vaccinal immunity to Marek's disease by infection with chicken anaemia agent[J].Avian Pathol,1998,17(2):333-347.
[20]蒋玲艳,韦平,李莉萍,等.鸡传染性贫血病毒与其他禽免疫抑制性疾病病毒的混合感染[J].中国兽医学报,2006,26(6):591-593.
[2]崔现兰,辛桂香,呈东来,等.鸡传染性贫血病毒的鉴定[J].中国畜禽传染病,1992(6):3-5.
[3]Zhou W,Shen B,Yang B,et al.Isolation and identification of chicken infectious anemia virus in China[J].Avian Dis,1997,41(2):361-364.
[4]Natesan S,Kataria J M,Dhama K,et al.Biological and molecular characterization of chicken anaemia virus isolates of Indian origin[J].Virus Res,2006,118(1-2):78-86.
[5]Kim H R,Kwon Y K,Bae Y C,et al.Molecular characterization of chicken infectious anemia viruses detected from breeder and broiler chickens in South Korea[J].Poult Sci,2010,89(11):2 426-2 431.
[6]Brown H K,Browning G F,Scott P C,et al.Full-length infectious clone of a pathogenic Australian isolate of chicken anaemia virus[J].Aust Vet J,2000,78(9):637-640.
[7]Schat K A.Chicken infectious anemia[C]//Saif YM(Ed),Diseases of Poultry,2003,11:182-202.Ames:Iowa State Press.
[8]McNulty M S,Mackie D P,Pollock D A,et al.Production and preliminary characterization of monoclonal antibodies to chicken anemia agent[J].Avian Dis,1991,34(2):352-358.
[9]卡尔尼克B W.禽病学[M].高福,苏敬良,译.10版.北京:中国农业大学出版社,1999:938-962.
[10]李延鹏,崔治中.一株鸡传染性贫血病毒野毒株致病性及其全基因组序列比较[J].微生物学报,2007,47(5):894-898.
[11]Zhang X H,Xie Q M,Ji J,et al.Complete genome sequence analysis of a recent chicken anaemia virus isolate and comparison with a chicken anemia virus isolatefrom human fecal samples in China[J].J Virol,2012,86(19):10896-10897.
[12]邵红霞,王平平,武敏,等.鸡传染性贫血病毒海安分离株感染雏鸡的病原动态分析[J].中国动物传染病学报,2012,20(5):1-7.
[13]曲鸿飞,李慧姣.鸡传染性贫血病毒研究进展[J].中国兽药杂志,2010,44(3):52-56.
[14]Adair B M,McNeilly F,McConnell C D,et al.Characterisation of surface markers present on cellsinfected by chicken anemia virus[J].Avian Dis,1993,37(4):943-950.
[15]刘忠贵,郑世民,徐彦波,等.鸡贫血因子病的免疫病理学变化[J].中国农业科学,1997,30(4):74-82.
[16]Cardona C,Lucio B,O′Connell P,et al.Humoral immune response to chicken infectious anemia virus in three strains of chickens in a closed flock[J].Avian Dis,2000,44(3):661-667.
[17]徐彦波,刘忠贵.鸡传染性贫血对雏鸡新城疫免疫的抑制机理(Ⅰ)[J].中国畜禽传染病,1994(5):38-40.
[18]周志勇,刘忠贵.鸡传染性贫血病毒感染鸡血清对骨髓造血祖细胞增殖功能的影响[J].中国畜禽传染病,1994(5):15-17.
[19]Otaaki Y,Nunoya T,Tajima M,et al.Depression of vaccinal immunity to Marek's disease by infection with chicken anaemia agent[J].Avian Pathol,1998,17(2):333-347.
[20]蒋玲艳,韦平,李莉萍,等.鸡传染性贫血病毒与其他禽免疫抑制性疾病病毒的混合感染[J].中国兽医学报,2006,26(6):591-593.