甜樱桃嫁接苗与组培苗枝条抗寒生理差异分析
|
摘要
为探明自然休眠期间甜樱桃枝条内抗寒相关的生理生化指标变化规律,筛选樱桃抗寒性的主要鉴定指标,并比较嫁接苗和组培苗休眠期间抗寒性的差异,以甜樱桃嫁接苗(早红宝石/大窝娄叶)和组培苗(早红宝石)为研究对象,对自然休眠过程中的一年生枝条中可溶性糖、淀粉、可溶性蛋白、游离脯氨酸、花青素、丙二醛(MDA)等含量,过氧化物歧化酶(SOD)活性等进行分析测定。结果表明,自然休眠过程中,枝条韧皮部中可溶性糖、淀粉、可溶性蛋白及MDA含量呈现先增加后降低,且体内保护酶SOD活性先增强再减弱,枝条韧皮部厚度与枝条电导率呈负相关,这些均可以作为樱桃抗寒性鉴定的指标,休眠期嫁接苗和组培苗抗寒生理指标多数取样点差异不显著。自然休眠过程中嫁接苗和组培苗枝条抗寒生理指标在休眠过程中出现相似的规律,嫁接苗与组培苗的抗寒性差异不显著。因此,考虑组培苗可以在嫁接苗栽培区试用推广。
The purpose of this study is to find out the difference of different kinds of sweet cherry branches ofhardiness and compare the difference of cold resistance between grafted seedlings and tissue culture seedlings duringdormancy. The sweet cherry biennial grafted seedlings potted(Zaohongbaoshi/Dawoluoye) and tissue cultureseedlings(Zaohongbaoshi) were used as the research objects. The content of soluble sugar, starch, soluble protein,proline and anthocyanin in 1-year old branches of sweet cherry grafted seedlings and tissue culture seedlings werestudied. The difference between superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in shootswere compared. The results indicated that soluble sugar, starch, soluble protein, MDA content and SOD activity in thebranches during natural dormancy present increased and then decreased, and branch phloem thickness and negativelycorrelated with conductivity branches.These indexes could be used as a cherry cold resistance identification criteria.Hibernating most grafting sampling time points and the somaclone cold resistance index difference were not significant.The cold resistance physiological indexes of grafted seedlings and tissue culture seedlings showed a similar trendduring dormancy. Cold resistance physiology indicators in grafting and tissue culturing seedlings of sweet cherry hadcertain differences, but no significant difference, therefore consider the tissue culturing seedlings spread in use throughthe grafting cultivation area.
The purpose of this study is to find out the difference of different kinds of sweet cherry branches ofhardiness and compare the difference of cold resistance between grafted seedlings and tissue culture seedlings duringdormancy. The sweet cherry biennial grafted seedlings potted(Zaohongbaoshi/Dawoluoye) and tissue cultureseedlings(Zaohongbaoshi) were used as the research objects. The content of soluble sugar, starch, soluble protein,proline and anthocyanin in 1-year old branches of sweet cherry grafted seedlings and tissue culture seedlings werestudied. The difference between superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in shootswere compared. The results indicated that soluble sugar, starch, soluble protein, MDA content and SOD activity in thebranches during natural dormancy present increased and then decreased, and branch phloem thickness and negativelycorrelated with conductivity branches.These indexes could be used as a cherry cold resistance identification criteria.Hibernating most grafting sampling time points and the somaclone cold resistance index difference were not significant.The cold resistance physiological indexes of grafted seedlings and tissue culture seedlings showed a similar trendduring dormancy. Cold resistance physiology indicators in grafting and tissue culturing seedlings of sweet cherry hadcertain differences, but no significant difference, therefore consider the tissue culturing seedlings spread in use throughthe grafting cultivation area.
引文
[1]董建军,冯文涛.栽培大樱桃应注意的技术问题.西北园艺(果树),2012(4):26-27.
[2]李勃,刘成连,杨瑞红,等.樱桃砧木抗寒性鉴定.果树学报,2006,23(2):196-199.
[3]陈秋芳,陈冲,马碧虎,等. 4种甜樱桃砧木的抗寒生理特性研究.山西果树,2013(6):3-5.
[4]Canli F A,Tian L. In vitro shoot regeneration from stored maturecotyledons of sweet cherry(Prunus avium L.)cultivars. ScientiaHorticulturae,2008,116(1):34-40.
[5]Godoy S,Tapia E,Seit P,et al. Temporary immersion systems for themass propagation of sweet cherry cultivars and cherry rootstocks:development of a micropropagation procedure and effect of cultureconditions on plant quality. In Vitro Cellular&DevelopmentalBiology-Plant,2017,53(5):494-504.
[6]吴瑕.樱桃抗寒生理与抗寒性鉴定指标研究.沈阳:沈阳农业大学,2004.
[7]吴禄平,吕德国,刘国成.甜樱桃无公害生产技术.北京:中国农业出版社,2002:24-26.
[8]沈洪波.杏品种抗寒性研究.泰安:山东农业大学,2002.
[9]罗小妹,王耀辉,丁春元,等.甘肃天水甜樱桃花期低温冻害情况调查.中国果树,2017(3):92-95.
[10]Salazar-Gutiérrez M R,Chaves B,Anothai J,et al. Variation in coldhardiness of sweet cherry flower buds through different phenologicalstages. Scientia Horticulturae,2014,172:161-167.
[11]陈新华,郭宝林,赵静,等.休眠期内甜樱桃不同品种枝条的抗寒性.河北农业大学学报,2009,32(6):37-40.
[12]罗玉兰,陆亮,王泰哲.本地和荷兰三色堇抗寒性的比较(简报).植物生理学通讯,2001,37(1):27-28.
[13]邹琦.植物生理学实验指导.北京:中国农业出版社,2000.
[14]郝建军,康宗利,于洋.植物生理学实验技术.北京:化学工业出版社,2007.
[15]张宪政,陈凤玉,王荣福.植物生理学实验技术.沈阳:辽宁科学技术出版社,1994,9(1):17-20.
[16]赵媛媛,刘明国,赵伟浩. 3种外来树种抗寒性生理指标的比较.安徽农业科学,2007,35(5):1298-1299.
[17]陈新华,郭宝林,赵静,等.甜樱桃休眠期枝条SOD·POD酶活性变化研究.安徽农业科学,2009,37(11):4958-4959.
[18]刘珅坤,刘庆忠,陈锋,等.甜樱桃砧木吉塞拉5抗寒性鉴定.落叶果树,2005,37(4):1-3.
[19]许祥明,叶和春,李国凤.脯氨酸代谢与植物抗渗透胁迫的研究进展.植物学报,2000,17(6):536-542.
[20]张基德,李玉梅,陈艳秋,等.梨品种枝条可溶性糖、脯氨酸含量变化规律与抗寒性的关系.延边大学农学学报,2004,26(4):281-285.
[21]Leyva A,Jarillo J A,Salinas J,et al. Low temperature inducesthe accumulation of phenylalanine ammonia-lyase and chalconesynthase mRNAs of Arabidopsis thaliana in a light-dependentmanner. Plant Physiology,1995,108(1):39-46.
[22]宁露云,包满珠,张蔚.低温胁迫对矮牵牛H株系花青素、游离脯氨酸及可溶性糖含量的影响.湖北农业科学,2016,55(6):1500-1503.
[23]Bhowmik P K,Tamura K,Sanada Y,et al. Sucrose metabolism ofperennial ryegrass in relation to cold acclimation. Zeitschrift FürNaturforschung C,2006,61(2):99-104.
[24]董胜豪,张钢,卻书鹏,等.抗寒锻炼期间白皮松电阻抗图谱参数对可溶性糖与淀粉含量变化的响应.河北农业大学学报,2009,32(3):53-58.
[25]范少然,崔睿航,武东霞,等.抗寒锻炼期间丰花月季茎的抗寒性及与可溶性糖淀粉的关系//中国园艺学会.中国观赏园艺研究进展.北京:中国林业出版社,2015:522-526.
[26]刘天明,张振文,李华,等.桃品种耐寒性研究.果树学报,1998(2):107-111.
[27]Lichev V,Papachatzis A. Influence of ten rootstocks on coldhardiness of flowers of cherry cultivar‘Bigarreau burlat’.Sodininkyste Ir Darininkyse,2006,25(3):296-301.
[28]郭学民,刘建珍,肖啸,等.桃、杏和樱桃树抗寒性研究进展.河北科技师范学院学报,2016,30(1):1-9.
[29]吴瑕,吕德国,刘坤.甜樱桃嫁接苗与组培苗抗寒性比较.沈阳农业大学学报,2005,36(1):93-95.
[2]李勃,刘成连,杨瑞红,等.樱桃砧木抗寒性鉴定.果树学报,2006,23(2):196-199.
[3]陈秋芳,陈冲,马碧虎,等. 4种甜樱桃砧木的抗寒生理特性研究.山西果树,2013(6):3-5.
[4]Canli F A,Tian L. In vitro shoot regeneration from stored maturecotyledons of sweet cherry(Prunus avium L.)cultivars. ScientiaHorticulturae,2008,116(1):34-40.
[5]Godoy S,Tapia E,Seit P,et al. Temporary immersion systems for themass propagation of sweet cherry cultivars and cherry rootstocks:development of a micropropagation procedure and effect of cultureconditions on plant quality. In Vitro Cellular&DevelopmentalBiology-Plant,2017,53(5):494-504.
[6]吴瑕.樱桃抗寒生理与抗寒性鉴定指标研究.沈阳:沈阳农业大学,2004.
[7]吴禄平,吕德国,刘国成.甜樱桃无公害生产技术.北京:中国农业出版社,2002:24-26.
[8]沈洪波.杏品种抗寒性研究.泰安:山东农业大学,2002.
[9]罗小妹,王耀辉,丁春元,等.甘肃天水甜樱桃花期低温冻害情况调查.中国果树,2017(3):92-95.
[10]Salazar-Gutiérrez M R,Chaves B,Anothai J,et al. Variation in coldhardiness of sweet cherry flower buds through different phenologicalstages. Scientia Horticulturae,2014,172:161-167.
[11]陈新华,郭宝林,赵静,等.休眠期内甜樱桃不同品种枝条的抗寒性.河北农业大学学报,2009,32(6):37-40.
[12]罗玉兰,陆亮,王泰哲.本地和荷兰三色堇抗寒性的比较(简报).植物生理学通讯,2001,37(1):27-28.
[13]邹琦.植物生理学实验指导.北京:中国农业出版社,2000.
[14]郝建军,康宗利,于洋.植物生理学实验技术.北京:化学工业出版社,2007.
[15]张宪政,陈凤玉,王荣福.植物生理学实验技术.沈阳:辽宁科学技术出版社,1994,9(1):17-20.
[16]赵媛媛,刘明国,赵伟浩. 3种外来树种抗寒性生理指标的比较.安徽农业科学,2007,35(5):1298-1299.
[17]陈新华,郭宝林,赵静,等.甜樱桃休眠期枝条SOD·POD酶活性变化研究.安徽农业科学,2009,37(11):4958-4959.
[18]刘珅坤,刘庆忠,陈锋,等.甜樱桃砧木吉塞拉5抗寒性鉴定.落叶果树,2005,37(4):1-3.
[19]许祥明,叶和春,李国凤.脯氨酸代谢与植物抗渗透胁迫的研究进展.植物学报,2000,17(6):536-542.
[20]张基德,李玉梅,陈艳秋,等.梨品种枝条可溶性糖、脯氨酸含量变化规律与抗寒性的关系.延边大学农学学报,2004,26(4):281-285.
[21]Leyva A,Jarillo J A,Salinas J,et al. Low temperature inducesthe accumulation of phenylalanine ammonia-lyase and chalconesynthase mRNAs of Arabidopsis thaliana in a light-dependentmanner. Plant Physiology,1995,108(1):39-46.
[22]宁露云,包满珠,张蔚.低温胁迫对矮牵牛H株系花青素、游离脯氨酸及可溶性糖含量的影响.湖北农业科学,2016,55(6):1500-1503.
[23]Bhowmik P K,Tamura K,Sanada Y,et al. Sucrose metabolism ofperennial ryegrass in relation to cold acclimation. Zeitschrift FürNaturforschung C,2006,61(2):99-104.
[24]董胜豪,张钢,卻书鹏,等.抗寒锻炼期间白皮松电阻抗图谱参数对可溶性糖与淀粉含量变化的响应.河北农业大学学报,2009,32(3):53-58.
[25]范少然,崔睿航,武东霞,等.抗寒锻炼期间丰花月季茎的抗寒性及与可溶性糖淀粉的关系//中国园艺学会.中国观赏园艺研究进展.北京:中国林业出版社,2015:522-526.
[26]刘天明,张振文,李华,等.桃品种耐寒性研究.果树学报,1998(2):107-111.
[27]Lichev V,Papachatzis A. Influence of ten rootstocks on coldhardiness of flowers of cherry cultivar‘Bigarreau burlat’.Sodininkyste Ir Darininkyse,2006,25(3):296-301.
[28]郭学民,刘建珍,肖啸,等.桃、杏和樱桃树抗寒性研究进展.河北科技师范学院学报,2016,30(1):1-9.
[29]吴瑕,吕德国,刘坤.甜樱桃嫁接苗与组培苗抗寒性比较.沈阳农业大学学报,2005,36(1):93-95.