中药复方及拆方的抗炎、镇痛、抑菌活性评价
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摘要
为了研究中药复方及其拆方的抗炎、镇痛、抑菌活性,本研究采用二甲苯致小鼠耳肿胀模型及冰醋酸致小鼠毛细血管渗透方法观察其对炎症的影响;采用醋酸致小鼠扭体试验及热板法观察其对疼痛的影响;采用二倍稀释法检测其对大肠杆菌、金黄色葡萄球菌、沙门氏菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。抗炎结果表明中药复方及拆方对二甲苯致炎小鼠的耳肿胀度和小鼠腹腔毛细血管通透性降低差异不显著(P>0.05);镇痛结果显示中药复方组、拆方都能极显著的减少小鼠扭体次数(P<0.01),但对热刺激引发的小鼠痛反应时间无明显的延长(P>0.05)作用;抑菌实验表明中药复方在0.63 mg/mL浓度以上时对大肠杆菌、金黄色葡萄球菌表现出很好的抑菌效果,对沙门氏菌呈中度敏感,其MIC为9.375 mg/mL,但拆方抑菌效果不明显。研究表明中药复方及其拆方组合具有一定的镇痛及抗菌活性。
In order to study the anti-inflammatory,analgesic and bacteriostatic effects of Chinese herbal compound and its disassemble prescription,it is used the acute inflammatory models such as xylene-induced ear edema and blood capillary leakage of abdominal cavity on mice to study inflammatory effect,and used acetic acid writhing method and hot plate method to study the analgesic effect on mice,and used trace double dilution method to measure the minimum inhibitory concentration( MIC) and minimal bactericidal concentration( MBC) of Escherichia coli,Staphylococcus aureus and Salmonella.The anti-inflammatory results showed Chinese herbal compound and its disassemble prescription could not extremely significantly reduce the degree of ear swelling of mice induced by xylene and the permeability of abdominal capillary( P>0.05).The analgesic results showed both Chinese herbal compound and its disassemble prescription could significantly reduce the number of writhing times( P<0.01),but there was no significant increase in the response time of mice induced by thermal stimulation( P>0.05).Bacteriostatic results showed Chinese herbal compound had better antibacterial effect on Escherichia coli and Staphylococcus aureus above the concentration of 0.63 mg/m L,and it was moderately sensitive to Salmonella with its MIC 9.375 mg/m L.However,its disassemble prescription inhibitory effect was not obvious.It is conclused Chinese herbal compound and its disassemble prescription has certain analgesic and antibacterial activity.
In order to study the anti-inflammatory,analgesic and bacteriostatic effects of Chinese herbal compound and its disassemble prescription,it is used the acute inflammatory models such as xylene-induced ear edema and blood capillary leakage of abdominal cavity on mice to study inflammatory effect,and used acetic acid writhing method and hot plate method to study the analgesic effect on mice,and used trace double dilution method to measure the minimum inhibitory concentration( MIC) and minimal bactericidal concentration( MBC) of Escherichia coli,Staphylococcus aureus and Salmonella.The anti-inflammatory results showed Chinese herbal compound and its disassemble prescription could not extremely significantly reduce the degree of ear swelling of mice induced by xylene and the permeability of abdominal capillary( P>0.05).The analgesic results showed both Chinese herbal compound and its disassemble prescription could significantly reduce the number of writhing times( P<0.01),but there was no significant increase in the response time of mice induced by thermal stimulation( P>0.05).Bacteriostatic results showed Chinese herbal compound had better antibacterial effect on Escherichia coli and Staphylococcus aureus above the concentration of 0.63 mg/m L,and it was moderately sensitive to Salmonella with its MIC 9.375 mg/m L.However,its disassemble prescription inhibitory effect was not obvious.It is conclused Chinese herbal compound and its disassemble prescription has certain analgesic and antibacterial activity.
引文
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[15]李娟,张学顺,傅春升.中药抗菌作用的研究进展[J].中国药业,2014,23(2):90-93.Li J,Zhang X S,Fu C S.Research progress of antibacterial action of Chinese herbal medicine[J].Chin Pharm,2014,23(2):90-93.
[16]张赛奇,王米,雷娜,等.中药对猪源大肠杆菌O8体外抑菌试验研究[J].家畜生态学报,2015,36(8):62-65.Zhang S Q,Wang M,Lei N,et al.Antibacterial Sensitivity of Chinese Herbal Medicine against Swine Escherichia coli O8 in Vitro[J].Journal of Domestic Animal Ecology,2015,36(8):62-65.
[17]Gundidza M,Sibanda M.Antimicrobial activities of ziziphus abyssinica and berchemia discolor[J].Central African Journal of Medicine,1991,37(3):80-83.
[2]陈立,董俊兴.勾儿茶属植物化学成分及其生物活性研究进展[J].中草药,2006,37(4):627-630.Chen L,Dong J X.The progress of chemical constituents from plants of Berchemia neck and Research on its biological activity[J].Chinese traditional and Herbal drugs,2006,37(4):627-630.
[3]代良敏,熊永爱,范奎,等.地榆化学成分与药理作用研究进展[J].中国实验方剂学杂志,2016,22(20):189-195.Dai L M,Xiong Y A,Fan K,et al.Chemical Composition and Pharmacological Effect of Sanguisorbae Radix[J].Chinese Journal of Experimental Traditional Medical Formulae.2016,22(20):189-195.
[4]赵崧,郑子春,沈洪.地榆、白芷、白蔹在溃疡性结肠炎大鼠中的作用及机制探讨[J].实用临床医药杂志,2011,15(7):1-4.Zhao S,Zhen Z C,Shen H.effects of radix sanguisorbae,radix angelicae dahuricae and cortex dictammni on the ulcerative colitis rats[J].Journal of Clinical Medicine in Practice|J Clin Med Prac.2011,15(7):1-4.
[5]王军伟,阮冰.乌药的植化及药理研究概况[J].浙江中医杂志,2006,41(11):675-677.Wang J W,Ruan B.The pharmacological study on the vegetation and plant of overview[J].Zhejiang Journal of Traditional Chinese Medicine,2006,41(11):675-677.
[6]王玉坤,李欣,冯将,等.家畜止痢散抗试验性腹泻及对胃肠运动的影响[J].黑龙江畜牧兽医,2016(20):163-165.Wang Y K,Li X,Feng J,et al.Effects of new domestic compound Zhilisan on experimental diarrhea and gastrointestinal motility in mice[J].Heilongjiang Animal Science and Veterinary Medicine,2016(20):163-165.
[7]王玉坤,冯将,魏玉好,等.止痢散及其拆方对胃肠运动的影响[J].中国兽药杂志,2016,50(7):57-61.Wang Y K,Feng J,Wei Y H,et al.Experiment Research in Anti-diarrhoea effects of Jiachu Zhilisan and its ingredients[J].Chinese Journal of Veterinary Drug,2016,50(7):57-61.
[8]史宁,郭宏举,王欢,等.金丝桃苷对急性炎症疼痛模型小鼠的抗炎镇痛作用研究[J].药物评价研究,2016,39(5):771-774.Shi N,Guo H J,Wang H,et al.Effects of hyperin on mice with acute inflammatory pain[J].Drug Evaluation Research,2016,39(5):771-774.
[9]张均田,杜冠华.现代药理实验方法[M].中国协和医科大学出版社,2012:2115-2117.Zhang J T,Du G H.Modern pharmacological experimental methods[M].Peking Union Medical College press,2012:2115-2117.
[10]李冀,于雪,马育轩,等.中药及其有效成分抗炎机制的研究进展[J].中医药学报,2010,38(2):134-137.Li J,Yu X,Ma Y X,et al.Research progress of anti-inflammatory mechanism of Chinese herbal medicine and its active ingredients[J].Acta Chinese Medicine and Pharmacology,2010,38(2):134-137.
[11]陆怡,朱元章,朱国福,等.中药镇痛机理研究概述[J].世界中医药,2015(4):629-632.Lu Y,Zhu Y Z,Zhu G F,et al.Research on Analgesia Mechanism of Traditional Chinese Medicine[J].World Chinese Medicine,2015,(4):629-632.
[12]俞浩,方艳夕,毛斌斌,等.地榆炮制前后水提物抗炎效果研究[J].中药材,2014,37(1):34-37.Yu H,Fang Y X,Mao B B,et al.Study on the water extract of Sanguisorba officinalis before and after processing the anti-inflammatory effect[J].Journal of Chinese Medicinal Materials,2014,37(1):34-37.
[13]汪群红,胡敏,汪官富.乌药生物碱镇痛和抗炎作用研究[J].中华中医药学刊,2015(4):910-912.Wang Q H,Hu M,Wang G F.Study on the analgesic and antiinflammatory effects of alkaloids of Radix Linderae[J].Chin Arch Tradit Chin Med,2015(4):910-912.
[14]陈希,穆祥,许剑琴.抗菌中药筛选思路的解析及实践[J].中国兽药杂志,2016,50(2):1-6.Chen X,Mu X,Xu J Q.Screening approach and practice of antimicrobial Chinese traditional medicine[J].Chinese Journal of Veterinary Drug,2016,50(2),1-6.
[15]李娟,张学顺,傅春升.中药抗菌作用的研究进展[J].中国药业,2014,23(2):90-93.Li J,Zhang X S,Fu C S.Research progress of antibacterial action of Chinese herbal medicine[J].Chin Pharm,2014,23(2):90-93.
[16]张赛奇,王米,雷娜,等.中药对猪源大肠杆菌O8体外抑菌试验研究[J].家畜生态学报,2015,36(8):62-65.Zhang S Q,Wang M,Lei N,et al.Antibacterial Sensitivity of Chinese Herbal Medicine against Swine Escherichia coli O8 in Vitro[J].Journal of Domestic Animal Ecology,2015,36(8):62-65.
[17]Gundidza M,Sibanda M.Antimicrobial activities of ziziphus abyssinica and berchemia discolor[J].Central African Journal of Medicine,1991,37(3):80-83.