戒毒一号对小鼠镇静催眠作用的实验研究
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摘要
目的:观察戒毒一号对C57BL/6小鼠的镇静催眠作用,为该药镇静催眠的临床研究提供实验依据。方法:连续15 d灌胃不同剂量的戒毒一号(14.12、56.48 g/kg),以生理盐水组为空白对照,艾司唑仑组为阳性对照。采用小鼠自主活动实验,观察戒毒一号对小鼠自发活动的中枢镇静作用;采用小鼠直接睡眠实验和协同阈上、阈下剂量戊巴比妥钠的睡眠实验,观察戒毒一号催眠作用。结果:高剂量戒毒一号可显著抑制小鼠自主活动(P<0.01),且没有直接睡眠作用(P>0.05),能够明显增加阈下剂量戊巴比妥钠诱导小鼠的入睡数及提高入睡率(P<0.01),对阈上剂量戊巴比妥钠诱导小鼠的睡眠潜伏期及睡眠时间有协同的趋势,但差异无统计学意义(P>0.05)。结论:戒毒一号有一定的镇静催眠作用,且随剂量的升高其镇静和催眠作用均有增强趋势。
Objective: To assess the sedative and hypnotic effects of JIEDUYIHAO on C57 BL/6 mice. Methods: The mice were randomly divided into four groups and orally administered with normal saline, estazolam and JIEDUYIHAO at daily doses of 14.12 and 56.48 g/kg respectively. After 15 days of consecutive administration,the autonomic activities test, sleep induced directly test, superthreshold and subthreshold doses of pentobarbital sodium test were used to evaluate the effect of JIEDUYIHAO on sleep behavior in mice. Results: JIEDUYIHAO(56.48 g/kg) could decrease the activities of mice significantly(P<0.01), and increase the number of mice which were made to sleep by subthreshold dose of pentobarbital sodium(P<0.01), while there was no directly sleep induced function of JIEDUYIHAO(P >0.05) and its effect on sleeping time of mice with superthreshold dose of pentobarbital sodium had no significant difference(P >0.05). Conclusion: JIEDUYIHAO shows dose-dependent sedative and hypnotic effects on mice.
Objective: To assess the sedative and hypnotic effects of JIEDUYIHAO on C57 BL/6 mice. Methods: The mice were randomly divided into four groups and orally administered with normal saline, estazolam and JIEDUYIHAO at daily doses of 14.12 and 56.48 g/kg respectively. After 15 days of consecutive administration,the autonomic activities test, sleep induced directly test, superthreshold and subthreshold doses of pentobarbital sodium test were used to evaluate the effect of JIEDUYIHAO on sleep behavior in mice. Results: JIEDUYIHAO(56.48 g/kg) could decrease the activities of mice significantly(P<0.01), and increase the number of mice which were made to sleep by subthreshold dose of pentobarbital sodium(P<0.01), while there was no directly sleep induced function of JIEDUYIHAO(P >0.05) and its effect on sleeping time of mice with superthreshold dose of pentobarbital sodium had no significant difference(P >0.05). Conclusion: JIEDUYIHAO shows dose-dependent sedative and hypnotic effects on mice.
引文
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[24]姜雪,孙森凤,王悦,等.甘草药理作用研究进展[J].化工时刊,2017,31(7):25-28.
[2] Roth T. Insomnia:definition, prevalence, etiology, and consequences[J].J Clin Sleep Med,2007,3(5 Suppl):S7-10.
[3] Herrmann E S, Johnson P S, Bruner N R, et al.Morning administration of oral methamphetamine dosedependently disrupts nighttime sleep in recreational stimulant users[J].Drug Alcohol Depend,2017(178):291-295.
[4] Atkin T,Comai S,Gobbi G. Drugs for Insomnia beyond Benzodiazepines:Pharmacology, Clinical Applications,and Discovery[J].Pharmacol Rev,2018,70(2):197-245.
[5]薛智慧,李洪亮,王欢欢,等.失眠临床治疗的研究进展[J].中医药导报,2014,20(16):55-57.
[6] Michelini S, Cassano G B, Frare F, et al. Long-term use of benzodiazepines:tolerance,dependence and clinical problems in anxiety and mood disorders[J].Pharmacopsychiatry,1996,29(4):127-134.
[7] Xu S Y,Bian R L,Chen X. Methodology of Pharmacological Experiment(药理实验方法学)[M].3th ed. Beijing:People’s Medical Publishing House,2002:802-806.
[8]李海静,高月,刘萍,等.失眠动物模型研究进展[J].中国药理学通报,2007,23(4):437-440.
[9]中华人民共和国卫生部.新药(西药)临床前研究指导原则汇编[M].北京:中华人民共和国卫生部药政局,1993.
[10]国家药典委员会.中国药典(一部)[M].北京:中国医药科技出版社,2015:92.
[11]唐希灿,金国章,胥彬.延胡索的药理研究Ⅶ.延胡索乙素对狗胃液分泌的影响及其催眠作用[J].药学学报,1962,9(3):145-150.
[12]金国章,郑秀凤,胥彬.延胡索的药理研究—Ⅻ.延胡索乙素旋光异构体的中枢作用[J].生理学报,1964,27(1):47-58.
[13]金国章,鄒岡,唐希燦,等.延胡索的药理研究Ⅵ.延胡索素乙对中枢神经系统的作用[J].生理学报,1960,24(2):110-120.
[14]杨英珍.延胡索素乙硫酸盐镇痛作用的初步临床观察(72例报告)[J].复旦学报(医学版),1959(6):577-581.
[15]黎发根,李绍旦,杨明会.中医药治疗失眠症作用机理研究进展[J].环球中医药,2012,5(7):543-546.
[16]刁远明,陈群.安寐汤对失眠大鼠脑组织多巴胺含量的影响[J].中国医药导报,2011,8(17):105-107.
[17]金国章.脑内多巴胺神经系统与四氢原小檗碱同类物药理作用的关系[J].生理科学进展,1993,24(2):182-185.
[18] Buda C, Sallanon M, Jin G Z. Effect of l-tetrahydropalmatine on sleep-waking cycle of cats[J].Zhongguo Yao Li Xue Bao,1984,5(1):5-8.
[19]侯崧,李文娟,冯光凌.黄芪与白术临床配伍机制及应用效果评价[J].亚太传统医药,2015,11(15):119-120.
[20]曾宇,张三印,胡冠英.当归补血汤的研究进展[J].时珍国医国药,2016,27(2):422-424.
[21]李明玉,徐煜彬,徐志立,等.茯苓改善学习记忆及镇静催眠作用研究[J].辽宁中医药大学学报,2014,16(5):25-26.
[22]覃甘梅,覃骊兰,蓝毓营.治疗失眠中药药对的应用原则[J].中医药导报,2018,24(5):23-26.
[23]张建军,胡春玲.中药党参研究的现代进展[J].甘肃高师学报,2017,22(3):39-43.
[24]姜雪,孙森凤,王悦,等.甘草药理作用研究进展[J].化工时刊,2017,31(7):25-28.