Effect of terahertz pulse on gene expression in human eye cells
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摘要
In recent years, the advances in terahertz applications have stimulated interest in the biological effects associated with this frequency range. We study the gene expression profile in three types of cells exposed to terahertz radiation,i.e., human ARPE-19 retinal pigment epithelial cells, simian virus 40-transformed human corneal epithelial cells, and human MIO-M1 Müller cells. We find that the gene expression in response to heat shock is unaffected, indicating that the minimum temperature increases under controlled environment. The transcriptome sequencing survey demonstrates that 6-hour irradiation with a broadband terahertz source results in specific change in gene expression and also the biological functions that are closely related to these genes. Our results imply that the effect of terahertz radiation on gene expression can last over 15 hours and depends on the type of cell.
In recent years, the advances in terahertz applications have stimulated interest in the biological effects associated with this frequency range. We study the gene expression profile in three types of cells exposed to terahertz radiation,i.e., human ARPE-19 retinal pigment epithelial cells, simian virus 40-transformed human corneal epithelial cells, and human MIO-M1 Müller cells. We find that the gene expression in response to heat shock is unaffected, indicating that the minimum temperature increases under controlled environment. The transcriptome sequencing survey demonstrates that 6-hour irradiation with a broadband terahertz source results in specific change in gene expression and also the biological functions that are closely related to these genes. Our results imply that the effect of terahertz radiation on gene expression can last over 15 hours and depends on the type of cell.
In recent years, the advances in terahertz applications have stimulated interest in the biological effects associated with this frequency range. We study the gene expression profile in three types of cells exposed to terahertz radiation,i.e., human ARPE-19 retinal pigment epithelial cells, simian virus 40-transformed human corneal epithelial cells, and human MIO-M1 Müller cells. We find that the gene expression in response to heat shock is unaffected, indicating that the minimum temperature increases under controlled environment. The transcriptome sequencing survey demonstrates that 6-hour irradiation with a broadband terahertz source results in specific change in gene expression and also the biological functions that are closely related to these genes. Our results imply that the effect of terahertz radiation on gene expression can last over 15 hours and depends on the type of cell.
引文
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[2]Lewis R A 2014 J.Phys.D:Appl.Phys.47 374001
[3]Wilmink G J and Grundt J E 2011 J.Infrared Millim.Terahertz Waves32 1074
[4]Wang Y,Ren Y,Chen L,Song C,Li C,Zhang C,Xu D and Yao J 2018Chin.Phys.B 27 114204
[5]Liu C,Wang C,Cao J 2016 Chin.Phys.B 25 028702
[6]Whitmire S E,Wolpert D,Markelz A G,Hillebrecht J R,Galan J and Birge R R 2003 Biophys.J.85 1269
[7]Fischer B M,Walther M and Uhd Jepsen P 2002 Phys.Med.Biol.473807
[8]Clothier R H and Bourne N 2003 J.Biol.Phys.29 179
[9]Hintzsche H,Jastrow C,Heinen B,Baaske K,Kleine-Ostmann T,Schwerdtfeger M,Shakfa M K,K¨arst U,Koch M,Schrader T and Stopper H 2013 Radiat.Res.179 38
[10]Amicis A D,Sanctis S D,Cristofaro S D,Franchini V,Lista F,Regalbuto E,Giovenale E,Gallerano G P,Nenzi P,Bei R,Fantini M,Benvenuto M,Masuelli L,Coluzzi E,Cicia C and Sgura A 2015 Mutat.Res.Genet.Toxicol.Environ.Mutagen.793 150
[11]Borovkova M,Serebriakova M,Fedorov V,Sedykh E,Vaks V,Lichutin A,Salnikova A and Khodzitsky M 2017 Biomed.Opt.Express 8 273
[12]Wilmink G J,Rivest B D,Roth C C,Ibey B L,Payne J A,Cundin L X,Grundt J E,Peralta X,Mixon D G and Roach W P 2011 Lasers Surg.Med.43 152
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[15]Alexandrov B S,Phipps M L,Alexandrov L B,Booshehri L G,Erat A,Zabolotny J,Mielke C H,Chen H,Rodriguez G,Rasmussen K?Martinez J S,Bishop A R and Usheva A 2013 Sci.Rep.3 1184
[16]Titova L V,Ayesheshim A K,Golubov A,Rodriguez-Juarez R,Woycicki R,Hegmann F A and Kovalchuk O 2013 Sci.Rep.3 2363
[17]Titova L V,Ayesheshim A K,Golubov A,Fogen D,Rodriguez-Juarez R,Hegmann F A and Kovalchuk O 2013 Biomed.Opt.Express 4 559
[18]Bogomazova A N,Vassina E M,Goryachkovskaya T N,Popik V M,Sokolov A S,Kolchanov N A,Lagarkova M A,Kiselev S L and Peltek S E 2015 Sci.Rep.5 7749
[19]Iomdina E N,Goltsman G N,Seliverstov S V,Sianosyan A A,Teplyakova K O and Rusova A A 2016 J.Biomed.Opt.21 97002
[20]Liu W,Lu Y,Jiao G,Chen X,Zhou Z,She R,Li J,Chen S,Dong Yand L¨u J 2016 Chin.Phys.B 25 060702
[21]Hebling J,Almasi G,Kozma I and Kuhl J 2002 Opt.Express 10 1161
[22]Kunitski M,Richter M,Thomson M D,Vredenborg A,Wu J,Jahnke T,Sch¨offler M,Schmidt-B¨ocking H,Roskos H G and D¨orner R 2013Opt.Express 21 6826
[23]Kristensen T T,Withayachumnankul W,Jepsen P U and Abbott D 2010Opt.Express 18 4727
[24]Zheng X,Cui H,Yin Y,Zhang Y,Zong R,Bao X,Ma J,Liu Z and Zhou Y 2017 Invest.Ophth.Vis.Sci.58 2874
[25]Wang Z,Gerstein M and Snyder M 2009 Nat.Rev.Genet.10 57
[26]Mortazavi A,Williams B A,McCue K,Schaeffer L and Wold B 2008Nat.Methods 5 621
[27]Robinson M D,McCarthy D J and Smyth G K 2010 Bioinformatics 26139
[28]Benjamini Y and Yekutieli Y 2001 Ann.Stat.29 1165
[29]Robinson D R,Wu Y M and Lin S F 2000 Oncogene 19 5548
[30]Zwick E,Bange J and Ullrich A 2001 Endocr.Relat.Cancer 8 161
[31]Belov A A and Mohammadi M 2013 Cold Spring Harb.Perspect.Biol.5 a015958
[32]Franchini V,Sanctis S D,Marinaccio J,Amicis A D,Coluzzi E,Cristofaro S D,Lista F,Regalbuto E,Doria A,Giovenale E,Gallerano G P,Bei R,Benvenuto M,Masuelli L,Udroiu I and Sgura A 2018 Environ.Mol.Mutagen.57 476
[33]Matlin A J,Clark F and Smith C W J 2005 Nat.Rev.Mol.Cell Bio.6386
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[35]Kulipanov G N,Gavrilov N G,Knyazev B A,Kolobanov E I,Kotenkov V V,Kubarev V V,Matveenko A N,Medvedev L E,Miginsky S V,Mironenko L A,Ovchar V K,Popik V M,Salikova T V,Scheglov MA,Serednyakov S S,Shevchenko O A,Skrinsky A N,Tcheskidov V Gand Vinokurov N A 2008 Terahertz Sci.Technol.1 107
[36]Homenko A,Kapilevich B,Kornstein R and Firer M A 2009 Bioelectromagnetics 30 167
[37]Frohlich H 1968 Int.J.Quantum Chem.2 641
[38]Alexandrov B S,Gelev V,Yoo S W,Alexandrov L B,Fukuyo Y,Bishop A R,?K and Usheva A 2010 Nucleic Acids Res.38 1790