同期发情处理的乏情奶牛血液病理学变化及其妊检阴性的风险预警
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摘要
为了探究乏情奶牛同期发情处理前血浆生化指标的变化及对其输精后28 d妊检阴性(-)的风险预警意义,本试验在黑龙江省某集约化牛场选择了产后实施同期发情的奶牛共48头,根据试验奶牛产后40 d内是否发情分为发情组(E)23头和乏情组(A)25头,并对同期发情前试验牛进行血浆生化指标分析和输精后28 d妊检。结果显示:与发情奶牛相比,乏情奶牛血浆中孕酮(P_4)含量显著升高;雌二醇(E_2)、葡萄糖(Glu)、硒(Se)、牛组胺(HIS)、脑源性神经营养因子(BDNF)、牛组胺(HIS)含量显著降低。应用Pearson相关分析和二元Logistic回归模型确定了应用三针法前当奶牛血浆HIS含量> 26.12μg/L,输精后28 d妊检为阴性(-)的风险增大;应用五针前当奶牛血浆中E_2含量<46.45 pmol/L时,输精后28 d妊检为阴性(-)的风险增大。
To explore the changes of plasma biochemical indicators before administration of timed artificial insemination(TAI) and the risk early warning of negative pregnancy at 28 d after insemination in anestrus cows.In this experiment,48 dairy cows from an intensive cattle farm in Heilongjiang province were selected to implement simultaneous estrus after calving.The experimental cattles were assigned into the estrus group(E,n=23) and the anestrus group(A,n=25) according to estrus behavior within postpartum 40 d.Plasma biochemical indicators were analyzed before administration of TAI and pregnancy test results were tracked at 28 d after insemination for test cows.The results showed that,compared with estrus cows,plasma concentration of P_4 was significantly increased in anestrus cows,while plasma estradiol(E_2),glucose(Glu),selenium(Se),cerebrogenic neurotrophic factors(BDNF)and cattle histamine(HIS)were significantly reduced before administration of TAI.Pearson correlation analysis and binary logistic regression model were used to determine when plasma HIS level was higher than 26.12 μg/L,the cows would get an increased risk of negative pregnancy at 28 days after Ovsynch and insemination.Similarly,when plasma E_(2 )content was less than 46.45 pmol/L,the cows would get an increased risk of negative pregnancy at 28 d after presynch-Ovsynch and insemination.
To explore the changes of plasma biochemical indicators before administration of timed artificial insemination(TAI) and the risk early warning of negative pregnancy at 28 d after insemination in anestrus cows.In this experiment,48 dairy cows from an intensive cattle farm in Heilongjiang province were selected to implement simultaneous estrus after calving.The experimental cattles were assigned into the estrus group(E,n=23) and the anestrus group(A,n=25) according to estrus behavior within postpartum 40 d.Plasma biochemical indicators were analyzed before administration of TAI and pregnancy test results were tracked at 28 d after insemination for test cows.The results showed that,compared with estrus cows,plasma concentration of P_4 was significantly increased in anestrus cows,while plasma estradiol(E_2),glucose(Glu),selenium(Se),cerebrogenic neurotrophic factors(BDNF)and cattle histamine(HIS)were significantly reduced before administration of TAI.Pearson correlation analysis and binary logistic regression model were used to determine when plasma HIS level was higher than 26.12 μg/L,the cows would get an increased risk of negative pregnancy at 28 days after Ovsynch and insemination.Similarly,when plasma E_(2 )content was less than 46.45 pmol/L,the cows would get an increased risk of negative pregnancy at 28 d after presynch-Ovsynch and insemination.
引文
[1] 李昌盛.奶牛产后乏情的主要致病因素调查及其血液临床病理学的研究[D].黑龙江大庆:黑龙江八一农垦大学,2015.
[2] 吴晨晨,夏成,张静,等.散栏式集约化牛场奶牛繁殖性能状况的调查[J].黑龙江畜牧兽医,2007(10):38-39.
[3] 朱玉哲.泌乳量与日粮能量水平对泌乳奶牛生殖机能的影响[D].黑龙江大庆:黑龙江八一农垦大学,2009.
[4] WILTBANK M C,PURSLEY J R.The cow as an induced ovulator:timed AI after synchronization of ovulation[J].Theriogenology,2014,81(1):170-185.
[5] KHATTI A,MEHROTRA S,PATEL P K,et al.Supplementation of vitamin E,selenium and increased energy allowance mitigates the transition stress and improves postpartum reproductive performance in the crossbred cow[J].Theriogenology,2017,104:142.
[6] KAFILZADEH F,KHEIRMANESH H,KARAMI S H,et al.Comparing the effect of oral supplementation of vitamin E,injective vitamin E and selenium or both during late pregnancy on production and reproductive performance and immune function of dairy cows and calves[J].Sci World J,2014(2):165841.
[7] 杜凤娇.脑源性神经营养因子对水牛卵母细胞体外成熟的影响及其作用机制的初步研究[D].广西南宁:广西大学,2015.
[8] SEIFER D B,FENG B O,SHELDEN R M,et al.Brain-derived neurotrophic factor:a novel human ovarian follicular protein[J].J Clin Endocrinol Met,2002,87(2):655-659.
[9] HONG S G,JANG G,OH H J,et al.The effects of brain-derived neurotrophic factor and metformin on in vitro developmental competence of bovine oocytes[J].Zygote,2009,17(3):187-193.
[10] 常晶,胡敏,张跃辉,等.肥大细胞及其介质在生殖系统中的作用[J].吉林医学,2015,36(7):1442-1443.
[11] 刘谚威,刘娜.肥大细胞及其介质组胺的生殖功能[J].上海畜牧兽医通讯,1992(5):36-37.
[12] 修云芳,王全溪,邵良平,等.小熊猫卵巢肥大细胞与雌性生殖干细胞的初步观察[J].2015,36(6):96-99.
[13] 周虚.哺乳对母牛产后发情排卵的调节作用[J].中国奶牛,1992(3):35-36.
[14] 张志胜,桑润滋.母牛产后乏情机理研究进展[J].黄牛杂志,1999(25):45-47.
[15] 吴晨晨,夏成,宋成,等.乏情奶牛日粮营养水平和血浆生殖激素的变化[J].中国奶牛,2008(5):34-36.
[16] WATHES D C,FENWICK M,CHENG Z,et al.Influence of negative energy balance on cyclicity and fertility in the high producing dairy cow[J].Theriogenology,2007,68(Suppl 1):S232-S241.
[17] LEROY J L,OPSOMER G,VAN S A,et al.Reduced fertility in high-yielding dairy cows:are the oocyte and embryo in danger?Part I.The importance of negative energy balance and altered corpus luteum function to the reduction of oocyte and embryo quality in high-yielding dairy cows[J].Reprod Domest Anim,2008,43(5):612-622.
[18] FURMAN M,WADE G N.Animal models in the study of nutritional infertility[J].Curr Opin Endocrinol,2007,14(6):475-481.
[19] KATZ M G,VOLLENHOVEN B.The reproductive endocrine consequences of anorexia nervosa[J].Bjog-Int J Obstet Gy,2000,107(6):707.
[20] 钱伟东.高产奶牛产后主要卵巢疾病的调查和血液临床病理学变化及其预警评估[D].黑龙江大庆:黑龙江八一农垦大学,2016.
[2] 吴晨晨,夏成,张静,等.散栏式集约化牛场奶牛繁殖性能状况的调查[J].黑龙江畜牧兽医,2007(10):38-39.
[3] 朱玉哲.泌乳量与日粮能量水平对泌乳奶牛生殖机能的影响[D].黑龙江大庆:黑龙江八一农垦大学,2009.
[4] WILTBANK M C,PURSLEY J R.The cow as an induced ovulator:timed AI after synchronization of ovulation[J].Theriogenology,2014,81(1):170-185.
[5] KHATTI A,MEHROTRA S,PATEL P K,et al.Supplementation of vitamin E,selenium and increased energy allowance mitigates the transition stress and improves postpartum reproductive performance in the crossbred cow[J].Theriogenology,2017,104:142.
[6] KAFILZADEH F,KHEIRMANESH H,KARAMI S H,et al.Comparing the effect of oral supplementation of vitamin E,injective vitamin E and selenium or both during late pregnancy on production and reproductive performance and immune function of dairy cows and calves[J].Sci World J,2014(2):165841.
[7] 杜凤娇.脑源性神经营养因子对水牛卵母细胞体外成熟的影响及其作用机制的初步研究[D].广西南宁:广西大学,2015.
[8] SEIFER D B,FENG B O,SHELDEN R M,et al.Brain-derived neurotrophic factor:a novel human ovarian follicular protein[J].J Clin Endocrinol Met,2002,87(2):655-659.
[9] HONG S G,JANG G,OH H J,et al.The effects of brain-derived neurotrophic factor and metformin on in vitro developmental competence of bovine oocytes[J].Zygote,2009,17(3):187-193.
[10] 常晶,胡敏,张跃辉,等.肥大细胞及其介质在生殖系统中的作用[J].吉林医学,2015,36(7):1442-1443.
[11] 刘谚威,刘娜.肥大细胞及其介质组胺的生殖功能[J].上海畜牧兽医通讯,1992(5):36-37.
[12] 修云芳,王全溪,邵良平,等.小熊猫卵巢肥大细胞与雌性生殖干细胞的初步观察[J].2015,36(6):96-99.
[13] 周虚.哺乳对母牛产后发情排卵的调节作用[J].中国奶牛,1992(3):35-36.
[14] 张志胜,桑润滋.母牛产后乏情机理研究进展[J].黄牛杂志,1999(25):45-47.
[15] 吴晨晨,夏成,宋成,等.乏情奶牛日粮营养水平和血浆生殖激素的变化[J].中国奶牛,2008(5):34-36.
[16] WATHES D C,FENWICK M,CHENG Z,et al.Influence of negative energy balance on cyclicity and fertility in the high producing dairy cow[J].Theriogenology,2007,68(Suppl 1):S232-S241.
[17] LEROY J L,OPSOMER G,VAN S A,et al.Reduced fertility in high-yielding dairy cows:are the oocyte and embryo in danger?Part I.The importance of negative energy balance and altered corpus luteum function to the reduction of oocyte and embryo quality in high-yielding dairy cows[J].Reprod Domest Anim,2008,43(5):612-622.
[18] FURMAN M,WADE G N.Animal models in the study of nutritional infertility[J].Curr Opin Endocrinol,2007,14(6):475-481.
[19] KATZ M G,VOLLENHOVEN B.The reproductive endocrine consequences of anorexia nervosa[J].Bjog-Int J Obstet Gy,2000,107(6):707.
[20] 钱伟东.高产奶牛产后主要卵巢疾病的调查和血液临床病理学变化及其预警评估[D].黑龙江大庆:黑龙江八一农垦大学,2016.