不同色光对蛇足石杉离体叶状体产HupA及内源激素水平的影响
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摘要
为探究不同色光对蛇足石杉离体叶状体生长和石杉碱甲(Hup A)累积的作用,设置了二极管白光(W)、二极管蓝光(B)、二极管红光(R) 3种不同色光处理,以普通荧光灯(CK)为对照,研究不同色光下叶状体生长和Hup A累积的差异及其与IAA、ZT、ABA、GA几种内源激素水平的关系.结果表明:红光处理对蛇足石杉离体叶状体生长繁殖有促进效果,但对Hup A含量累积有极显著的抑制作用;蓝光相较CK提高了叶状体的生长量和Hup A的含量,其叶状体Hup A生产率最高达1 811. 728μg·L~(-1),较CK提高了49. 67%;蓝光处理的叶状体IAA/ZT值、ABA含量、ABA/ZT值、ABA/IAA值在全生育期均高于CK.依据不同类型激素功能,该研究结果说明:蓝光培养的叶状体中存在高水平的生长型激素IAA/ZT,促进其生长,增加了生物量,且其体内成熟型激素ABA的高水平存在,促进了次级代谢产物Hup A累积增加.
For exploring the effect of different colored lights on the growth and Huperzine A accumulation of the thallus of Huperzia serrata,set up several different colored lights treatment including diode white light( W),diode blue light( B),diode red light( R) are set up to treat. Compared with the common fluorescent lamp( CK),and studied the difference of the growth and HupA accumulation of the thallus under different colored lights and the relationship between them and endogenous hormone including IAA,ZT and ABA,GA are studied. The results show that red light is helpful to promote the growth and proliferation of the thallus of Huperzia serrata in vitro,but it had an extremely significant inhibitory effect on content of HupA. Compared with CK,blue light treatment increase the growth amount and HupA content of the thallus,and the HupA productivity is highest,up to 1 811. 728 μg·L~(-1),which is higher 49. 67% than CK. The total IAA/ZT value,the ABA content,ABA/ZT value,ABA/TAA value of the thallus treated with diode blue light are all higher than CK during full period. Depending on the function of different types of hormones,the results of this study indicate that there is high level of growth hormone IAA/ZT in the thallus treated with blue light,which promotes its growth and increased the biomass,and the high level of ABA promotes the accumulation of secondary metabolite HupA.
For exploring the effect of different colored lights on the growth and Huperzine A accumulation of the thallus of Huperzia serrata,set up several different colored lights treatment including diode white light( W),diode blue light( B),diode red light( R) are set up to treat. Compared with the common fluorescent lamp( CK),and studied the difference of the growth and HupA accumulation of the thallus under different colored lights and the relationship between them and endogenous hormone including IAA,ZT and ABA,GA are studied. The results show that red light is helpful to promote the growth and proliferation of the thallus of Huperzia serrata in vitro,but it had an extremely significant inhibitory effect on content of HupA. Compared with CK,blue light treatment increase the growth amount and HupA content of the thallus,and the HupA productivity is highest,up to 1 811. 728 μg·L~(-1),which is higher 49. 67% than CK. The total IAA/ZT value,the ABA content,ABA/ZT value,ABA/TAA value of the thallus treated with diode blue light are all higher than CK during full period. Depending on the function of different types of hormones,the results of this study indicate that there is high level of growth hormone IAA/ZT in the thallus treated with blue light,which promotes its growth and increased the biomass,and the high level of ABA promotes the accumulation of secondary metabolite HupA.
引文
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[14]韩邦兴,陈乃富,代剑平,等.光照对蛇足石杉某些生理生化指标的影响[J].植物资源与环境学报,2009,18(3):88-90.
[15]陈曼,涂艺声,叶丽婻,等.氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响[J].植物学报,2017,52(2):218-224.
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[18]王海波,王帅,王孝娣,等.光质对设施葡萄叶片衰老与内源激素含量的影响[J].应用生态学报,2017,28(11):3535-3543.
[2]蔡龚莉,方帅,张起辉.蛇足石杉的药学研究进展[J].天然产物研究与开发,2015,27(5):931-939.
[3]林如辉,刘美龙.中草药蛇足石杉的研究概况[J].海峡药学,2013,25(9):21-25.
[4]Ferreira A,Rodrigues M,Fortuna A,et al.Huperzine Afrom Huperzia serrata:a review of its sources,chemistry,pharmacology and toxicology[J].Phytochemistry Reviews,2016,15(1):51-85.
[5]Ding Rui,Sun Bingfeng,Lin Guoqiang.An efficient total synthesis of(-)-Huperzine A[J].Organic Letters,2012,14(17):4446-4449.
[6]White J D,Li Yang,Kim J,et al.A novel synthesis of(-)-Huperzine A via tandem intramolecular aza-prins cyclization-cyclobutane fragmentation[J].Organic Letters,2013,15(4):882-885.
[7]Shu Shaohua,Zhao Xinmei,Wang Wenjuan,et al.Identification of a novel endophytic fungus from Huperzia serrata which produces Huperzine A[J].World Journal of Microbiology and Biotechnology,2014,30(12):3101-3109.
[8]李晓君,杨雪飞,朱皖南.蛇足石杉茎尖组织培养研究[J].中国现代中药,2014,16(5):387-390.
[9]吉枝单.蛇足石杉离体叶状体生产石杉碱甲的工艺条件研究[D].南昌:江西师范大学,2015.
[10]张小红,李晓君,杨雪飞.不同条件对蛇足石杉组培苗生长及石杉碱甲积累的影响[J].中国现代中药,2016,18(4):472-477.
[11]黄馨凤,李万成.蛇足石杉的生态环境研究进展[J].中国林副特产,2011(2):73-76.
[12]石玮,罗建平,赵晓丹.影响九华山千层塔石杉碱甲含量的主要环境因子分析[J].植物资源与环境学报,2008,17(3):58-62.
[13]Liu Yang,Fang Shengzuo,Yang Wanxia,et al.Light quality affects flavonoid production and related gene expression in Cyclocarya paliurus[J].Journal of Photochemistry and Photobiology B:Biology,2018,179:66-73.
[14]韩邦兴,陈乃富,代剑平,等.光照对蛇足石杉某些生理生化指标的影响[J].植物资源与环境学报,2009,18(3):88-90.
[15]陈曼,涂艺声,叶丽婻,等.氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响[J].植物学报,2017,52(2):218-224.
[16]王昭清.不同光质的LED光源对鱼腥草生理生化及次生代谢的影响[D].太原:山西师范大学,2017.
[17]刘慧雯.LED光质对铁皮石斛组培拟原球茎和幼苗生长及主要有效成分的影响[D].济南:山东农业大学,2017.
[18]王海波,王帅,王孝娣,等.光质对设施葡萄叶片衰老与内源激素含量的影响[J].应用生态学报,2017,28(11):3535-3543.