外源一氧化氮对干旱胁迫下烤烟脂膜过氧化的抑制效应
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摘要
为探究一氧化氮(NO)在干旱胁迫下对烤烟脂膜过氧化的抑制效应,采用盆栽试验研究了不同浓度NO供体硝普钠(sodium nitroprusside,SNP)对干旱胁迫下烤烟叶片和根系膜透性、MDA含量、抗氧化酶活性及渗透调节物质含量的影响。结果表明:干旱胁迫下,烤烟叶片和根系膜透性,丙二醛(malondialdehyde,MDA)、可溶性蛋白、可溶性糖、脯氨酸(proline,Pro)、K+含量以及超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)活性明显提升,过氧化氢酶(catalase,CAT)显著降低;喷施0.25 mmol/L和0.50 mmol/L SNP分别使烤烟叶片、根系膜透性和MDA含量显著降低,抗氧化酶活性和渗透调节物质含量显著增加。说明外源SNP能有效提高烤烟抗氧化酶活性和渗透调节物质含量,维持细胞膜系统稳定性,缓解干旱胁迫对烤烟细胞脂膜的氧化损伤,同时SNP在缓解干旱损伤时具有浓度效应,且烤烟不同器官对SNP浓度响应不同。
In order to explore the inhibitory effects of nitric oxide on lipid peroxidation in flue-cured tobacco under drought stress,the pot experiment was carried out to investigate the effects of different concentrations of exogenous NO donor( sodium nitroprusside,SNP) on the membrane permeability of leaf and root system of flue-cured tobacco,MDA content,antioxidant enzyme activity and content of osmotic adjustment substances under drought stress. The results showed that membrane permeability of leaf and root system,the contents of MDA,soluble protein,soluble sugar,proline( Pro) and K+,and the activities of superoxide dismutase(SOD) and catalase(POD) of the leaves and roots of tobacco increased significantly under drought stress,while the content of catalase( CAT) decreasedsignificantly. The membrane permeability of leaf and root system and MDA content decreased significantly after spraying 0.25 mmol/L and 0.5 mmol/L,while the activity of antioxidant enzymes and content of osmotic adjustment substances increased significantly. This indicated that exogenous SNP could effectively improve the activity of antioxidant enzymes and content of osmotic adjustment substances,maintain the stability of cell membrane,and alleviate the oxidative damage of lipid membranes of flue cured tobacco under drought stress. It also showed that the concentration effect of SNP on alleviating drought damage,and different organs of flue-cured tobacco had different response to SNP concentration.
In order to explore the inhibitory effects of nitric oxide on lipid peroxidation in flue-cured tobacco under drought stress,the pot experiment was carried out to investigate the effects of different concentrations of exogenous NO donor( sodium nitroprusside,SNP) on the membrane permeability of leaf and root system of flue-cured tobacco,MDA content,antioxidant enzyme activity and content of osmotic adjustment substances under drought stress. The results showed that membrane permeability of leaf and root system,the contents of MDA,soluble protein,soluble sugar,proline( Pro) and K+,and the activities of superoxide dismutase(SOD) and catalase(POD) of the leaves and roots of tobacco increased significantly under drought stress,while the content of catalase( CAT) decreasedsignificantly. The membrane permeability of leaf and root system and MDA content decreased significantly after spraying 0.25 mmol/L and 0.5 mmol/L,while the activity of antioxidant enzymes and content of osmotic adjustment substances increased significantly. This indicated that exogenous SNP could effectively improve the activity of antioxidant enzymes and content of osmotic adjustment substances,maintain the stability of cell membrane,and alleviate the oxidative damage of lipid membranes of flue cured tobacco under drought stress. It also showed that the concentration effect of SNP on alleviating drought damage,and different organs of flue-cured tobacco had different response to SNP concentration.
引文
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[3]G9bel C,Feussner I.Methods for the analysis of oxylipins in plants[J].Phytochemistry,2009,70(70):1485-1503.
[4]荣智媛,张晓海,杨双龙,等.抗氧化系统参与循环干旱锻炼提高烟草植株抗旱性的形成[J].植物生理学报,2012,48(7):705-713.Rong Z Y,Zhang X H,Yang S L,et al..Involvement of antioxidant defense system in enhancement of drought resistance in tobacco(Nicotiana tabacum L.)plants through circular drought hardening[J].Acta Phytophysiol.Sin.,2012,48(7):705-713.
[5]黄国宾,张晓海,杨双龙,等.渗透调节参与循环干旱锻炼提高烟草植株抗旱性的形成[J].植物生理学报,2012,48(5):465-471.Huang G B,Zhang X H,Yang S L,et al..Involvement of osmotic regulation in enhancement of drought resistance in tobacco(Nicotiana tabacum L.)plants through circular drought hardening[J].Acta Phytophysiol.Sin.,2012,48(5):465-471.
[6]Qiao W H,Fan L M.Nitric oxide signaling in plant responses to abiotic stresses[J].J.Integrat.Plant Biol.,2008,50(10):1238-1246.
[7]陈静,刘连涛,孙红春,等.外源NO对缺氮胁迫下棉花幼苗形态及生长的调控效应[J].中国农业科学,2014,47(23):4595-4605.Chen J,Liu L T,Sun H C,et al..Regulatory effects of exogenous nitric oxide on morphology of cotton seedlings under nitrogen stress[J].Sci.Agric.Sin.,2014,47(23):4595-4605.
[8]杨永杰,杨雪芹,张彩霞,等.干旱胁迫下NO对水稻日本晴叶片生理特性的影响[J].中国水稻科学,2015,29(1):65-72.Yang Y J,Yang X Q,Zhang C X,et al..Effects of nitric oxide on drought stress-induced physiological characteristics in leaves of nipponbare(Oryza sativa L.)[J].Chin.J.Rice Sci.,2015,29(1):65-72.
[9]周万海,冯瑞章,师尚礼,等.NO对盐胁迫下苜蓿根系生长抑制及氧化损伤的缓解效应[J].生态学报,2015,35(11):3606-3614.Zhou W H,Feng R Z,Shi S L,et al..Nitric oxide protection of alfalfa seedling roots against salt-induced inhibition of growth and oxidative[J].Acta Ecol.Sin.,2015,35(11):3606-3614.
[10]尚宏芹,高昌勇.外源NO对高温胁迫下辣椒幼苗生长和生理指标的影响[J].核农学报,2015,29(8):1617-1623.Shang H Q,Gao C Y.Effects of exogenous nitric oxide on growth and physiological indexes of pepper seedlings under high temperature stress[J].J.Nucl.Agric.Sci.,2015,29(8):1617-1623.
[11]朱涵毅,陈益军,劳佳丽,等.外源NO对镉胁迫下水稻幼苗抗氧化系统和微量元素积累的影响[J].生态学报,2013,33(2):603-609.Zhu H Y,Chen Y J,Lao J L,et al..The effect of exogenous nitric oxide on activities of antioxidant enzymes and microelements accumulation of two rice genotypes seedlings under cadmium stress[J].Acta Ecol.Sin.,2013,33(2):603-609.
[12]刘建新,王金成,贾海燕,等.外源NO对渗透胁迫下黑麦草种子萌发及活性氧代谢的影响[J].干旱地区农业研究,2012,30(6):128-133.Liu J X,Wang J C,Jia H Y,et al..Effects of exogenous nitric oxide on seed germination and reactive oxygen species metabolism of seedling leaves in ryegrass under osmotic stress[J].Agric.Res.Arid Areas,2012,30(6):128-133.
[13]朱再标,宁梓君,郭巧生,等.外源一氧化氮对干旱胁迫下白花蛇舌草种子萌发的影响[J].中草药,2014,45(6):840-843.Zhu Z B,Ning Z J,Guo Q S,et al..Effect of exogenous nitric oxide on seed germination of Oldenlandia diffusa under drought stress[J].Chin.Trad.Herbal Drugs,2014,45(6):840-843.
[14]郑春芳,姜东,戴廷波,等.外源一氧化氮供体硝普钠浸种对盐胁迫下小麦幼苗碳氮代谢及抗氧化系统的影响[J].生态学报,2010,30(5):1174-1183.Zheng C F,Jiang D,Dai T B,et al..Effects nitroprusside,a nitric oxide donor,on carbon and nitrogen metabolism and the activity of the antioxidation system in wheat seedings under salt stress[J].Acta Ecol.Sin.,2010,30(5):1174-1183.
[15]郝岗平,杜希华,史仁玖.干旱胁迫下外源一氧化氮促进银杏可溶性糖、脯氨酸和次生代谢产物合成[J].植物生理与分子生物学学报,2007(6):499-506.Hao G P,Du X H,Shi R J.Exogenous nitric oxide accelerates soluble sugar,proline and secondary metabolite synthesis in ginkgo biloba under drought stress[J].J.Plant Physiol.Mol.Biol.,2007,33(6):499-506.
[16]陈银萍,蘧苗苗,苏向楠,等.外源一氧化氮对镉胁迫下紫花苜蓿幼苗活性氧代谢和镉积累的影响[J].农业环境科学学报,2015,34(12):2261-2271.Chen Y P,Qu M M,Su X N,et al..Effect of exogenous nitric oxide on active oxygen metabolism and cadmium accumulation in alfalfa seeding under cadmium stress[J].J.Agro-Environ.Sci.,2015,34(12):2261-2271.
[17]吴雪霞,朱月林,朱为民,等.外源一氧化氮对Na Cl胁迫下番茄幼苗光合作用和离子含量的影响[J].植物营养与肥料学报,2007,13(4):658-663.Wu X X,Zhu Y L,Zhu W M,et al..Effects of exogenous nitric oxide on photosynthesis and ionic contents of tomato seedlings under Na Cl stress[J].Plant Nutr.Fertil.Sci.,2007,13(4):658-663.
[18]Zhao L Q,Zhuang F,Guo J K,et al..Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed[J].Plant Physiol.,2004,134(2):849-857.
[19]Uchida A,Jagendorf A T,Hibino T,et al..Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice[J].Plant Sci.,2002,163(3):515-523.
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