盆栽试验中外源NO对干旱胁迫下烤烟光合日变化和营养元素含量的影响
|
摘要
为揭示一氧化氮(NO)对干旱胁迫下烤烟耐旱性影响的内在机制,采用盆栽试验研究了NO供体硝普钠(Sodium nitroprusside,SNP)在干旱胁迫条件下对烤烟叶绿素含量(质量分数)、光合日变化、光合同化产物和营养元素含量(质量分数)的影响。结果表明,干旱胁迫下烤烟叶绿素(Chl)含量显著下降,净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)明显降低,胞间CO_2浓度(Ci)明显升高,烟株出现光合"午休"现象,同时可溶性糖和蔗糖含量显著增加,淀粉含量下降,P、Ca、Mg、Fe和Mn含量显著降低,N、K含量显著增加,但对B、Zn和Cu影响不大;外源SNP(0.25mmol/L)使干旱胁迫下烤烟Chl显著增加,Pn、Gs、Tr明显提高,Ci明显降低,光合"午休"现象消失,可溶性糖含量进一步提高,但蔗糖含量显著降低,淀粉的分解减少。同时NO能提高干旱胁迫下烤烟叶片中P、K、Ca、Mg、Fe和Mn含量。因此,外源NO能在一定程度上缓解干旱胁迫对烤烟光合同化能力和营养元素吸收的不利影响,提高烤烟的耐旱性。
To reveal the mechanism of nitric oxide(NO) on drought tolerance of flue-cured tobacco under drought stress, a pot experiment was carried out to study the effects of sodium nitroprusside(SNP, an exogenous NO donor) on the chlorophyll content, diurnal variation of photosynthesis, photosynthetic products and nutrient element contents of flue-cured tobacco under drought stress. The results showed that under drought stress, the chlorophyll(Chl) content in flue-cured tobacco significantly decreased. The net photosynthetic rate(Pn), stomatal conductance(Gs) and transpiration rate(Tr) was reduced, while the intercellular CO_2 concentration(Ci) increased with apparent midday depression of photosynthesis. Simultaneously, the contents of soluble sugar, sucrose,N,and K significantly increased, and the contents of starch, P, Ca, Mg, Fe and Mn significantly reduced. In addition, the contents of B, Zn and Cu were changed slightly. Under drought stress, the application of exogenous SNP(0.25mmol/L) significantly increased the Chl content in flue-cured tobacco,promoted the Pn, Gs and Tr, and decreased its Ci content. The midday depression of photosynthesis suppressed.The contents of soluble sugar, P, K, Ca, Mg, Fe and Mn raised, however, the sucrose content was significantly reduced, and starch decomposition diminished. Therefore, the exogenous NO could alleviate the adverse effects of drought stress on the capacity of photosynthetic assimilation and the nutrient element absorption of fluecured tobacco and improve its drought tolerance to a certain extent.
To reveal the mechanism of nitric oxide(NO) on drought tolerance of flue-cured tobacco under drought stress, a pot experiment was carried out to study the effects of sodium nitroprusside(SNP, an exogenous NO donor) on the chlorophyll content, diurnal variation of photosynthesis, photosynthetic products and nutrient element contents of flue-cured tobacco under drought stress. The results showed that under drought stress, the chlorophyll(Chl) content in flue-cured tobacco significantly decreased. The net photosynthetic rate(Pn), stomatal conductance(Gs) and transpiration rate(Tr) was reduced, while the intercellular CO_2 concentration(Ci) increased with apparent midday depression of photosynthesis. Simultaneously, the contents of soluble sugar, sucrose,N,and K significantly increased, and the contents of starch, P, Ca, Mg, Fe and Mn significantly reduced. In addition, the contents of B, Zn and Cu were changed slightly. Under drought stress, the application of exogenous SNP(0.25mmol/L) significantly increased the Chl content in flue-cured tobacco,promoted the Pn, Gs and Tr, and decreased its Ci content. The midday depression of photosynthesis suppressed.The contents of soluble sugar, P, K, Ca, Mg, Fe and Mn raised, however, the sucrose content was significantly reduced, and starch decomposition diminished. Therefore, the exogenous NO could alleviate the adverse effects of drought stress on the capacity of photosynthetic assimilation and the nutrient element absorption of fluecured tobacco and improve its drought tolerance to a certain extent.
引文
[1]汪耀富,张福锁.干旱和氮用量对烤烟干物质和矿质养分积累的影响[J].中国烟草学报,2003,9(1):19-23,29.WANG Yaofu,ZHANG Fusuo.Effects of drought stress and nitrogen on dry matter accumulation and concentration of mineral elements in flue-cured tobacco[J].Acta Tabacaria Sinica,2003,9(1):19-23,29.
[2]蔡海霞,吴福忠,杨万勤.干旱胁迫对高山柳和沙棘幼苗光合生理特征的影响[J].生态学报,2011,31(9):2430-2436.CAI Haixia,WU Fuzhong,YANG Wanqin.Effects of drought stress on the photosynthesis of Salix paraqplesia and Hippophae rhamnoides seedlings[J].Acta Ecologica Sinica,2011,31(9):2430-2436.
[3]汪耀富,孙德梅,徐传快,等.干旱胁迫对烤烟养分吸收分配及产量品质的影响[J].干旱地区农业研究,2006,24(1):65-69.WANG Yaofu,SUN Demei,XU Chuankuai,et al.Effects of drought stress on absorption and distribution of nutrients and yield and quality of flue-cured tobacco[J].Agricultural Research in the Arid Areas,2006,24(1):65-69.
[4]Qiao W H,Fan L M.Nitric oxide signaling in plant responses to abiotic stresses[J].Journal of Integrative Plant Biology,2008,50(10):1238-1246.
[5]陈静,刘连涛,孙红春,等.外源NO对缺氮胁迫下棉花幼苗形态及生长的调控效应[J].中国农业科学,2014,47(23):4595-4605.CHEN Jing,LIU Liantao,SUN Hongchun,et al.Regulatory effects of exogenous nitric oxide on morphology of cotton seedlings under nitrogen stress[J].Scientia Agricultura Sinica,2014,47(23):4595-4605.
[6]杨永杰,杨雪芹,张彩霞,等.干旱胁迫下NO对水稻日本晴叶片生理特性的影响[J].中国水稻科学,2015,29(1):65-72.YANG Yongjie,YANG Xueqin,ZHANG Caixia,et al.Effects of nitric oxide on drought stress-induced physiological characteristics in leaves of nipponbare(Oryza sativa L.)[J].Chinese Journal of Rice Science,2015,29(1):65-72.
[7]周万海,冯瑞章,师尚礼,等.NO对盐胁迫下苜蓿根系生长抑制及氧化损伤的缓解效应[J].生态学报,2015,35(11):3606-3614.ZHOU Wanhai,FENG Ruizhang,SHI Shangli,et al.Nitric oxide protection of alfalfa seedling roots against salt-induced inhibition of growth and oxidative damage[J].Acta Ecologica Sinica,2015,35(11):3606-3614.
[8]尚宏芹,高昌勇.外源NO对高温胁迫下辣椒幼苗生长和生理指标的影响[J].核农学报,2015,29(8):1617-1623.SHANG Hongqin,GAO Changyong.Effects of exogenous nitric oxide on growth and physiological indexes of pepper seedlings under high temperature stress[J].Journal of Nuclear Agricultural Science,2015,29(8):1617-1623.
[9]朱涵毅,陈益军,劳佳丽,等.外源NO对镉胁迫下水稻幼苗抗氧化系统和微量元素积累的影响[J].生态学报,2013,33(2):603-609.ZHU Hanyi,CHEN Yijun,LAO Jiali,et al.The effect of exogenous nitric oxide on activities of antioxidant enzymes and microelements accumulation of two rice genotypes seedlings under cadmium stress[J].Acta Ecologica Sinica,2013,33(2):603-609.
[10]吴雪霞,朱月林,朱为民,等.外源一氧化氮对Na Cl胁迫下番茄幼苗光合作用和离子含量的影响[J].植物营养与肥料学报,2007,13(4):658-663.WU Xuexia,ZHU Yuelin,ZHU Weimin,et al.Effects of exogenous nitric oxide on photosynthetic characteristics of tomato seedlings under Na Cl stress[J].Plant Nutrition and Fertilizer Science,2007,13(4):658-663.
[11]邵瑞鑫,信龙飞,杨青华,等.NO对干旱条件下小麦幼苗PSII功能特性的调节效应[J].作物学报,2012,38(9):1710-1715.SHAO Ruixin,XIN Longfei,YANG Qinghua,et al.Modulation of exogenous nitric oxide on photosystemⅡfunctions in wheat seedlings under drought stress[J].Acta Agronomica Sinica,2012,38(9):1710-1715.
[12]郝岗平,杜希华,史仁玖.干旱胁迫下外源一氧化氮促进银杏可溶性糖、脯氨酸和次生代谢产物合成[J].植物生理与分子生物学学报,2007,33(6):499-506.HAO Gangping,DU Xihua,SHI Renjiu.Exogenous nitric oxide accelerates soluble sugar,proline and secondary metabolite synthesis in Ginkgo biloba under drought stress[J].Journal of Plant Physiology and Molecular Biology,2007,33(6):499-506.
[13]闻玉,赵翔,张骁.水分胁迫下一氧化氮对小麦幼苗根系生长和吸收的影响[J].作物学报,2008,34(2):344-348.WEN Yu,ZHAO Xiang,ZHANG Xiao.Effects of nitric oxide on root growth and absorption in wheat seedlings in response to water stress[J].Acta Agronomica Sinica,2008,34(2):344-348.
[14]刘柿良,潘远智,杨容孑,等.外源一氧化氮对镉胁迫下长春花质膜过氧化、ATPase及矿质营养吸收的影响[J].植物营养与肥料学报,2014,20(2):445-458.LIU Shiliang,PAN Yuanzhi,YANG Rongjie,et al.Effects of exogenous NO on mineral nutrition absorption,lipid peroxidation and ATPase of plasma membrane in Catharanthus roseus tissues under cadmium stress[J].Journal of Plant Nutrition and Fertilizer,2014,20(2):445-458.
[15]魏明月,云菲,刘国顺,等.Ca Cl2对强光胁迫下烤烟光合特性的影响[J].中国烟草学报,2016,22(3):37-43.WEI Mingyue,YUN Fei,LIU Guoshun,et al.Effects of Ca Cl2on photosynthetic characteristics of flue-cured tobacco under intense light stress[J].Acta Tabacaria Sinica,2016,22(3):37-43.
[16]YC/T 161—2002烟草及烟草制品总氮的测定连续流动法[S].YC/T 161—2002 Tobacco and tobacco products—Determination of total nitrogen—Continuous flow method[S].
[17]许冬青,彭黔荣,杨敏,等.烟草中淀粉测定方法研究现状与探讨[J].耕作与栽培,2011(3):54-56,53.XU Dongqing,PENG Qianrong,YANG Min,et al.Research status and discussion on the determination of starch in tobacco[J].Tillage and Cultivation,2011(3):54-56,53.
[18]薛应龙.植物生理学实验手册[M].上海:上海科学技术出版社,1985:135-138.
[19]张国芝.凯氏(Kjeldahl)定氮法之实验总结[J].山东化工,2011,40(1):57-59.ZHANG Guozhi.The experimental summary of Kjeldahl nitrogen determination methods[J].Shandong Chemical Industry,2011,40(1):57-59.
[20]赵铭钦,李元实,刘友杰,等.不同基因型烤烟生长期间叶片矿质元素的差异分析[J].华北农学报,2010,25(1):199-203.ZHAO Mingqin,LI Yuanshi,LIU Youjie,et al.Analysis on the difference of mineral nutrition content in leaves of different flue-cured tobacco genotypes during growing period[J].Acta Agriculturae BorealiSinica,2010,25(1):199-203.
[21]吴甘霖,段仁燕,王志高,等.干旱和复水对草莓叶片叶绿素荧光特性的影响[J].生态学报,2010,30(14):3941-3946.WU Ganlin,DUAN Renyan,WANG Zhigao,et al.Effects of drought stress and rehydration on chlorophyll fluorescence characteristics in Fragaria×ananassa Duch[J].Acta Ecologica Sinica,2010,30(14):3941-3946.
[22]黄国宾,张晓海,杨双龙,等.渗透调节参与循环干旱锻炼提高烟草植株抗旱性的形成[J].植物生理学报,2012,48(5):465-471.HUANG Guobin,ZHANG Xiaohai,YANG Shuanglong,et al.Involvement of osmotic regulation in enhancement of drought resistance in tobacco(Nicotiana tabacum L.)plants through circular droughthardening[J].Plant Physiology Journal,2012,48(5):465-471.
[23]Waraich E A,Ahmad R,Ashraf M Y.Role of mineral nutrition in alleviation of drought stress in plants[J].Australian Journal of Crop Science,2011,5(6):764-777.
[24]董顺德,张延春,孙德梅,等.干旱胁迫下烤烟矿质养分含量与烟叶产、质量的关系[J].烟草科技,2005(2):30-34.DONG Shunde,ZHANG Yanchun,SUN Demei,et al.Relationship between content of mineral nutrients and yield and quality of flue-cured tobacco under drought stress[J].Tobacco Science&Technology,2005(2):30-34.
[25]Mata C G,Lamattina L.Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress[J].Plant Physiology,2001,126(3):1196-1204.
[26]杨美森,王雅芳,干秀霞,等.外源一氧化氮对冷害胁迫下棉花幼苗生长、抗氧化系统和光合特性的影响[J].中国农业科学,2012,45(15):3058-3067.YANG Meisen,WANG Yafang,GAN Xiuxia,et al.Effects of exogenous nitric oxide on growth,antioxidant system and photosynthetic characteristics in seedling of cotton cultivar under chilling injury stress[J].Scientia Agricultura Sinica,2012,45(15):3058-3067.
[27]刘建新,王金成,王瑞娟,等.干旱胁迫下一氧化氮对燕麦幼苗生长和生理特性的影响[J].中国草地学报,2015,37(2):41-45.LIU Jianxin,WANG Jincheng,WANG Ruijuan,et al.Effects of nitric oxide on growth and physiological characteristics of oat seedlings under drought stress[J].Chinese Journal of Grassland,2015,37(2):41-45.
[28]刘柿良,杨容孑,潘远智,等.外源NO对镉胁迫下长春花质膜过氧化ATP酶活性及光合特性的影响[J].农业环境科学学报,2013,32(12):2360-2368.LIU Shiliang,YANG Rongjie,PAN Yuanzhi,et al.Effects of exogenous nitric oxide on lipid peroxidation and ATPase activity in plasma membrane and photosynthetic characteristics of Catharanthus roseus under cadmium stress[J].Journal of Agro-Environment Science,2013,32(12):2360-2368.
[29]Cui X M,Zhang Y K,Wu X B,et al.The investigation of the alleviated effect of copper toxicity by exogenous nitric oxide in tomato plants[J].Plant Soil&Environment,2010,56(6):274-281.
[2]蔡海霞,吴福忠,杨万勤.干旱胁迫对高山柳和沙棘幼苗光合生理特征的影响[J].生态学报,2011,31(9):2430-2436.CAI Haixia,WU Fuzhong,YANG Wanqin.Effects of drought stress on the photosynthesis of Salix paraqplesia and Hippophae rhamnoides seedlings[J].Acta Ecologica Sinica,2011,31(9):2430-2436.
[3]汪耀富,孙德梅,徐传快,等.干旱胁迫对烤烟养分吸收分配及产量品质的影响[J].干旱地区农业研究,2006,24(1):65-69.WANG Yaofu,SUN Demei,XU Chuankuai,et al.Effects of drought stress on absorption and distribution of nutrients and yield and quality of flue-cured tobacco[J].Agricultural Research in the Arid Areas,2006,24(1):65-69.
[4]Qiao W H,Fan L M.Nitric oxide signaling in plant responses to abiotic stresses[J].Journal of Integrative Plant Biology,2008,50(10):1238-1246.
[5]陈静,刘连涛,孙红春,等.外源NO对缺氮胁迫下棉花幼苗形态及生长的调控效应[J].中国农业科学,2014,47(23):4595-4605.CHEN Jing,LIU Liantao,SUN Hongchun,et al.Regulatory effects of exogenous nitric oxide on morphology of cotton seedlings under nitrogen stress[J].Scientia Agricultura Sinica,2014,47(23):4595-4605.
[6]杨永杰,杨雪芹,张彩霞,等.干旱胁迫下NO对水稻日本晴叶片生理特性的影响[J].中国水稻科学,2015,29(1):65-72.YANG Yongjie,YANG Xueqin,ZHANG Caixia,et al.Effects of nitric oxide on drought stress-induced physiological characteristics in leaves of nipponbare(Oryza sativa L.)[J].Chinese Journal of Rice Science,2015,29(1):65-72.
[7]周万海,冯瑞章,师尚礼,等.NO对盐胁迫下苜蓿根系生长抑制及氧化损伤的缓解效应[J].生态学报,2015,35(11):3606-3614.ZHOU Wanhai,FENG Ruizhang,SHI Shangli,et al.Nitric oxide protection of alfalfa seedling roots against salt-induced inhibition of growth and oxidative damage[J].Acta Ecologica Sinica,2015,35(11):3606-3614.
[8]尚宏芹,高昌勇.外源NO对高温胁迫下辣椒幼苗生长和生理指标的影响[J].核农学报,2015,29(8):1617-1623.SHANG Hongqin,GAO Changyong.Effects of exogenous nitric oxide on growth and physiological indexes of pepper seedlings under high temperature stress[J].Journal of Nuclear Agricultural Science,2015,29(8):1617-1623.
[9]朱涵毅,陈益军,劳佳丽,等.外源NO对镉胁迫下水稻幼苗抗氧化系统和微量元素积累的影响[J].生态学报,2013,33(2):603-609.ZHU Hanyi,CHEN Yijun,LAO Jiali,et al.The effect of exogenous nitric oxide on activities of antioxidant enzymes and microelements accumulation of two rice genotypes seedlings under cadmium stress[J].Acta Ecologica Sinica,2013,33(2):603-609.
[10]吴雪霞,朱月林,朱为民,等.外源一氧化氮对Na Cl胁迫下番茄幼苗光合作用和离子含量的影响[J].植物营养与肥料学报,2007,13(4):658-663.WU Xuexia,ZHU Yuelin,ZHU Weimin,et al.Effects of exogenous nitric oxide on photosynthetic characteristics of tomato seedlings under Na Cl stress[J].Plant Nutrition and Fertilizer Science,2007,13(4):658-663.
[11]邵瑞鑫,信龙飞,杨青华,等.NO对干旱条件下小麦幼苗PSII功能特性的调节效应[J].作物学报,2012,38(9):1710-1715.SHAO Ruixin,XIN Longfei,YANG Qinghua,et al.Modulation of exogenous nitric oxide on photosystemⅡfunctions in wheat seedlings under drought stress[J].Acta Agronomica Sinica,2012,38(9):1710-1715.
[12]郝岗平,杜希华,史仁玖.干旱胁迫下外源一氧化氮促进银杏可溶性糖、脯氨酸和次生代谢产物合成[J].植物生理与分子生物学学报,2007,33(6):499-506.HAO Gangping,DU Xihua,SHI Renjiu.Exogenous nitric oxide accelerates soluble sugar,proline and secondary metabolite synthesis in Ginkgo biloba under drought stress[J].Journal of Plant Physiology and Molecular Biology,2007,33(6):499-506.
[13]闻玉,赵翔,张骁.水分胁迫下一氧化氮对小麦幼苗根系生长和吸收的影响[J].作物学报,2008,34(2):344-348.WEN Yu,ZHAO Xiang,ZHANG Xiao.Effects of nitric oxide on root growth and absorption in wheat seedlings in response to water stress[J].Acta Agronomica Sinica,2008,34(2):344-348.
[14]刘柿良,潘远智,杨容孑,等.外源一氧化氮对镉胁迫下长春花质膜过氧化、ATPase及矿质营养吸收的影响[J].植物营养与肥料学报,2014,20(2):445-458.LIU Shiliang,PAN Yuanzhi,YANG Rongjie,et al.Effects of exogenous NO on mineral nutrition absorption,lipid peroxidation and ATPase of plasma membrane in Catharanthus roseus tissues under cadmium stress[J].Journal of Plant Nutrition and Fertilizer,2014,20(2):445-458.
[15]魏明月,云菲,刘国顺,等.Ca Cl2对强光胁迫下烤烟光合特性的影响[J].中国烟草学报,2016,22(3):37-43.WEI Mingyue,YUN Fei,LIU Guoshun,et al.Effects of Ca Cl2on photosynthetic characteristics of flue-cured tobacco under intense light stress[J].Acta Tabacaria Sinica,2016,22(3):37-43.
[16]YC/T 161—2002烟草及烟草制品总氮的测定连续流动法[S].YC/T 161—2002 Tobacco and tobacco products—Determination of total nitrogen—Continuous flow method[S].
[17]许冬青,彭黔荣,杨敏,等.烟草中淀粉测定方法研究现状与探讨[J].耕作与栽培,2011(3):54-56,53.XU Dongqing,PENG Qianrong,YANG Min,et al.Research status and discussion on the determination of starch in tobacco[J].Tillage and Cultivation,2011(3):54-56,53.
[18]薛应龙.植物生理学实验手册[M].上海:上海科学技术出版社,1985:135-138.
[19]张国芝.凯氏(Kjeldahl)定氮法之实验总结[J].山东化工,2011,40(1):57-59.ZHANG Guozhi.The experimental summary of Kjeldahl nitrogen determination methods[J].Shandong Chemical Industry,2011,40(1):57-59.
[20]赵铭钦,李元实,刘友杰,等.不同基因型烤烟生长期间叶片矿质元素的差异分析[J].华北农学报,2010,25(1):199-203.ZHAO Mingqin,LI Yuanshi,LIU Youjie,et al.Analysis on the difference of mineral nutrition content in leaves of different flue-cured tobacco genotypes during growing period[J].Acta Agriculturae BorealiSinica,2010,25(1):199-203.
[21]吴甘霖,段仁燕,王志高,等.干旱和复水对草莓叶片叶绿素荧光特性的影响[J].生态学报,2010,30(14):3941-3946.WU Ganlin,DUAN Renyan,WANG Zhigao,et al.Effects of drought stress and rehydration on chlorophyll fluorescence characteristics in Fragaria×ananassa Duch[J].Acta Ecologica Sinica,2010,30(14):3941-3946.
[22]黄国宾,张晓海,杨双龙,等.渗透调节参与循环干旱锻炼提高烟草植株抗旱性的形成[J].植物生理学报,2012,48(5):465-471.HUANG Guobin,ZHANG Xiaohai,YANG Shuanglong,et al.Involvement of osmotic regulation in enhancement of drought resistance in tobacco(Nicotiana tabacum L.)plants through circular droughthardening[J].Plant Physiology Journal,2012,48(5):465-471.
[23]Waraich E A,Ahmad R,Ashraf M Y.Role of mineral nutrition in alleviation of drought stress in plants[J].Australian Journal of Crop Science,2011,5(6):764-777.
[24]董顺德,张延春,孙德梅,等.干旱胁迫下烤烟矿质养分含量与烟叶产、质量的关系[J].烟草科技,2005(2):30-34.DONG Shunde,ZHANG Yanchun,SUN Demei,et al.Relationship between content of mineral nutrients and yield and quality of flue-cured tobacco under drought stress[J].Tobacco Science&Technology,2005(2):30-34.
[25]Mata C G,Lamattina L.Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress[J].Plant Physiology,2001,126(3):1196-1204.
[26]杨美森,王雅芳,干秀霞,等.外源一氧化氮对冷害胁迫下棉花幼苗生长、抗氧化系统和光合特性的影响[J].中国农业科学,2012,45(15):3058-3067.YANG Meisen,WANG Yafang,GAN Xiuxia,et al.Effects of exogenous nitric oxide on growth,antioxidant system and photosynthetic characteristics in seedling of cotton cultivar under chilling injury stress[J].Scientia Agricultura Sinica,2012,45(15):3058-3067.
[27]刘建新,王金成,王瑞娟,等.干旱胁迫下一氧化氮对燕麦幼苗生长和生理特性的影响[J].中国草地学报,2015,37(2):41-45.LIU Jianxin,WANG Jincheng,WANG Ruijuan,et al.Effects of nitric oxide on growth and physiological characteristics of oat seedlings under drought stress[J].Chinese Journal of Grassland,2015,37(2):41-45.
[28]刘柿良,杨容孑,潘远智,等.外源NO对镉胁迫下长春花质膜过氧化ATP酶活性及光合特性的影响[J].农业环境科学学报,2013,32(12):2360-2368.LIU Shiliang,YANG Rongjie,PAN Yuanzhi,et al.Effects of exogenous nitric oxide on lipid peroxidation and ATPase activity in plasma membrane and photosynthetic characteristics of Catharanthus roseus under cadmium stress[J].Journal of Agro-Environment Science,2013,32(12):2360-2368.
[29]Cui X M,Zhang Y K,Wu X B,et al.The investigation of the alleviated effect of copper toxicity by exogenous nitric oxide in tomato plants[J].Plant Soil&Environment,2010,56(6):274-281.