珠海口岸地区孕妇人群梅毒螺旋体基因分型的初步研究
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摘要
目的分析珠海口岸地区孕妇人群的梅毒螺旋体感染情况及基因分型,探讨先天性梅毒与梅毒基因分型的关系。方法统计2016―2017年该院建档孕妇的梅毒检测例数为4 414例,其中42例被确诊为现状梅毒,其新生儿有9例患有先天性梅毒。对梅毒阳性患者血液进行巢式PCR检测arp、tpr基因并进行分型。结果 42例现状梅毒孕妇有25例检测出polA基因阳性,9例先天性梅毒患儿检测出7例polA基因阳性;最后综合基因分型14a型2例(6%,2/32),13d型5例(16%,5/32),14d型25例(78%,25/32),其中7例polA基因阳性的先天性患儿和母亲的型别全为14d型。结论珠海口岸地区梅毒流行基因型主要为14d型,同样在先天性梅毒中基因型也是14d型。
Objective To analyze the status and genotyping of Treponema pallidum infection in pregnant women in Zhuhai port area,and to investigate the relationship between congenital syphilis and syphilis genotyping.Methods The number of syphilis test cases of pregnant women in our hospital was 4 414 from 2016 to2017,of which 42 cases were diagnosed with current syphilis,their newborn babies,and 9 cases with congenital syphilis.Nest PCR was used to detect the meltox arp gene and tpr gene in the blood of syphilis positive patients and to classify them.Results A total of 25 cases of pregnant women with syphilis positive for polA gene,and 9 cases of congenital syphilis tested positive for polA gene in 7 cases.The final comprehensive gene classification type 14 ahad two cases(6%,2/32),13 dtype had 5 cases(16%,5/32),14 dtype had 25 cases(78%,25/32).Among them,seven cases of congenital children and mothers with polA gene positive were all type 14 d.Conclusion Epidemic genotype of Zhuhai port area syphilis is mainly 14 d,as well as in congenital syphilis genotype.
Objective To analyze the status and genotyping of Treponema pallidum infection in pregnant women in Zhuhai port area,and to investigate the relationship between congenital syphilis and syphilis genotyping.Methods The number of syphilis test cases of pregnant women in our hospital was 4 414 from 2016 to2017,of which 42 cases were diagnosed with current syphilis,their newborn babies,and 9 cases with congenital syphilis.Nest PCR was used to detect the meltox arp gene and tpr gene in the blood of syphilis positive patients and to classify them.Results A total of 25 cases of pregnant women with syphilis positive for polA gene,and 9 cases of congenital syphilis tested positive for polA gene in 7 cases.The final comprehensive gene classification type 14 ahad two cases(6%,2/32),13 dtype had 5 cases(16%,5/32),14 dtype had 25 cases(78%,25/32).Among them,seven cases of congenital children and mothers with polA gene positive were all type 14 d.Conclusion Epidemic genotype of Zhuhai port area syphilis is mainly 14 d,as well as in congenital syphilis genotype.
引文
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[11]王林娜,樊硕,郑和义,等.外周血检测梅毒螺旋体DNA及其分子亚型的初步观察[J].中华医学杂志,2014,94(12):928-931.
[12]杨文生,杨文林,杨健.梅毒PCR诊断与梅毒螺旋体基因分型研究进展[J].广东医学杂志,2010,31(19):2597-2599.
[2]PILLAY A,LIU H,CHEN C Y,et al.Molecular subtyping of Treponema pallidum subspecies pallidum[J].Sex Transm Dis,1998,25(8):408-414.
[3]曾铁兵,吴移谋,黄澍杰,等.衡阳和江门地区梅毒螺旋体基因分型的初步研究[J].中华皮肤科杂志,2004,37(12):692-694.
[4]郑和平,欧志英,胡玉山,等.梅毒螺旋体的巢式PCR检测与基因分型[J].中华皮肤科杂志,2005,38(9):408-414.
[5]曾铁兵,吴移谋,黄澍杰,等.巢式PCR扩增梅毒螺旋体pol Ae及其临床应用的研究[J].中国皮肤性病学杂志,2004,18(2):74-76.
[6]吴奇,王丽,季灵婷,等.上海闸北地区妊娠梅毒分子流行病学特征分析[J].检验医学杂志,2016,31(6):486-490.
[7]汤镇.梅毒螺旋体基因分型及临床相关性研究进展[J].临床医学与临床杂志,2016,13(3):420-422.
[8]杨文生,杨文林,杨健,等.梅毒血清固定与梅毒螺旋体trp基因亚型关系的初步研究[J].中国现代医学杂志,2011,21(16):1821-1825.
[9]FLORINDO C,REIGADO V,GOMES J P,et al.Molecular typing of Treponema pallidum clinical strains from Lisbon,Portugal[J].J Clin Microbiol,2008,46(11):3802-3803.
[10]CASTRO R,PRIETO E,AGUAS M J,et al.Molecular subtyping of Treponema pallidum subsp.Pallidum in Lisbon,Portugal[J].J Clin Microbiol,2009,47(8):2510-2512.
[11]王林娜,樊硕,郑和义,等.外周血检测梅毒螺旋体DNA及其分子亚型的初步观察[J].中华医学杂志,2014,94(12):928-931.
[12]杨文生,杨文林,杨健.梅毒PCR诊断与梅毒螺旋体基因分型研究进展[J].广东医学杂志,2010,31(19):2597-2599.