某复合膳食纤维对小鼠通便功能的影响研究
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摘要
目的观察复合膳食纤维对小鼠通便功能的影响。方法 2013年10月根据《保健食品检验与评价技术规范》通便功能评价方法,将SPF昆明种雄性小鼠(体重18~22g)按体重随机分为2组,每组50只。每组再等量分为5组:空白对照组、模型对照组和0.5、1.0、3.0g/kg·bw剂量的复合膳食纤维组。连续灌胃15d后测定小肠墨汁推进率、排首粒黑便时间、5h内排粪便粒数和粪便重量。结果各剂量组对小鼠体重增长无明显影响(P>0.05);高剂量组小鼠的小肠墨汁推进率(92.70±5.34)%较模型对照组(60.46±16.13)%显著提高(P<0.01);低、中、高剂量组排首粒黑便时间及排便粒数分别为(163.54±42.79)、(143.16±31.73)、(143.43±44.71)min和(3.18±2.03)、(3.47±2.52)、(3.64±2.86)粒,各剂量组均能明显缩短排首粒黑便时间(P<0.01),增加排便粒数(P<0.05)。结论复合膳食纤维具有通便功能。
Objective To observe the effect of a compound dietary fiber on the catharsis function in mice.Methods In October 2013,in accordance with the method for the assessment of catharsis function in Technical Standards for Testing&Assessment of Health Food.100SPF Kunming male mice(18g-22g)were randomly divided into two groups,50in each group.Then each group was randomly divided into five equal groups(the blank control group,the model control group and compound dietary fiber groups of 0.5g/kg·bw,1.0g/kg·bw,and 3.0g/kg·bw).The ink propelling rate in small intestine,the time of first defecate,the quantity and the weight of mouse feces within 5hours were determined after continuously intragastric infusion for 15days.Results The compound dietary fiber groups had no significant effect on the weight increase in mice(P>0.05);The ink propelling rate of small intestine in high-dose group(92.70±5.34)%significantly increased than the model control group(60.46±16.13)%(P<0.01),The time of first defecate was(163.54±42.79),(143.16±31.73)and(143.43±44.71)min in the low,medium and high dose groups,and the number of mice feces were(3.18±2.03),(3.47±2.52)and(3.64±2.86);All the three dose groups could greatly shorten the time of first defecate(P<0.01),and increase the number of mice feces(P<0.05).Conclusion The compound dietary fiber has the catharsis function.
Objective To observe the effect of a compound dietary fiber on the catharsis function in mice.Methods In October 2013,in accordance with the method for the assessment of catharsis function in Technical Standards for Testing&Assessment of Health Food.100SPF Kunming male mice(18g-22g)were randomly divided into two groups,50in each group.Then each group was randomly divided into five equal groups(the blank control group,the model control group and compound dietary fiber groups of 0.5g/kg·bw,1.0g/kg·bw,and 3.0g/kg·bw).The ink propelling rate in small intestine,the time of first defecate,the quantity and the weight of mouse feces within 5hours were determined after continuously intragastric infusion for 15days.Results The compound dietary fiber groups had no significant effect on the weight increase in mice(P>0.05);The ink propelling rate of small intestine in high-dose group(92.70±5.34)%significantly increased than the model control group(60.46±16.13)%(P<0.01),The time of first defecate was(163.54±42.79),(143.16±31.73)and(143.43±44.71)min in the low,medium and high dose groups,and the number of mice feces were(3.18±2.03),(3.47±2.52)and(3.64±2.86);All the three dose groups could greatly shorten the time of first defecate(P<0.01),and increase the number of mice feces(P<0.05).Conclusion The compound dietary fiber has the catharsis function.
引文
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[2]王娟,汪雨亭,杨公明.香蕉不溶性膳食纤维的理化特性与通便功能研究[J].食品工业科技,2017,(2):337-341.
[3]刘海宁.肠道菌群与功能性便秘的研究进展[J].复旦学报(医学版),2015,42(4):564-568.
[4]黄志弟,赵学尧,陈先翰,等.功能性便秘的中医研究进展[J].广西中医学院学报,2012,15(2):71-72.
[5]金鑫,高擎,葛亚中.一种中草药组方保健食品润肠通便功能的研究[J].现代食品科技,2016,32(1):77-81.
[6]严维娜,黄俊明.一种不溶性膳食纤维的润肠通便作用效果观察[J].广东卫生防疫,200,27(4):28-29.
[7]刘宏.菊粉的功能特性与开发应用[J].中国食物与营养,2010,(12):25-27.