TWEAK/Fn14在子宫内膜异位症的表达及意义
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摘要
目的研究肿瘤坏死因子样凋亡微弱诱导剂(TWEAK)和成纤维细胞因子诱导14(Fn14)在子宫内膜异位症(EMs)的表达及意义。方法用实时定量逆转录(RT-PCR)和免疫组化检测子宫内膜异位症患者异位病灶(ECE组,22例)、在位内膜(EUE组,26例)中TWEAK和Fn14 mRNA和蛋白的表达,并与正常组(CE组,40例)子宫内膜对比。结果TWEAK/Fn14 mRNA和蛋白均表达于子宫内膜,EMs患者异位病灶的TWEAK的m RNA和蛋白表达均显著低于在位内膜和对照组正常子宫内膜(P<0.05),而Fn14的mRNA和蛋白表达均显著高于在位内膜和对照组正常子宫内膜(P<0.05)。结论 TWEAK低表达可促进子宫内膜间质细胞增生及转移,Fn14高表达可代偿性降低TWEAK,这种异常表达,提示TWEAK/Fn14参与了EMs的发生发展过程。
Objective To investigate the expression and significance of TWEAK and Fn14 in endometriosis.Methods mRNA and protein expression of TWEAK and Fn14 in the ectopic ednometrial tissues(ECE group,22 patients),the matched eupotic endometrial tissues(EUE group) and control endometial tissues(CE group) were detected by real-time PCR and immunohistochemical staining.Results mRNA and protein of TWEAK/Fn14 were expressed in the endometrial tissues.mRNA and protein expression of TWEAK were significantly lower in the ECE group when compared with the EUE group and the CE group(P<0.05).mRNA and protein expression of Fn14 were significantly higher in the ECE group when compared with the EUE group and the CE group(P<0.05).Conclusions The decreased expression of TWEAK maybe promote the endometrial cell proliferation and metastasis.The increased expression of Fn14 can be compensated to reduce the expression of TWEAK.The abnormal expression indicated TWEAK and Fn14 may be involved in the occurrence and development of endometriosis.
Objective To investigate the expression and significance of TWEAK and Fn14 in endometriosis.Methods mRNA and protein expression of TWEAK and Fn14 in the ectopic ednometrial tissues(ECE group,22 patients),the matched eupotic endometrial tissues(EUE group) and control endometial tissues(CE group) were detected by real-time PCR and immunohistochemical staining.Results mRNA and protein of TWEAK/Fn14 were expressed in the endometrial tissues.mRNA and protein expression of TWEAK were significantly lower in the ECE group when compared with the EUE group and the CE group(P<0.05).mRNA and protein expression of Fn14 were significantly higher in the ECE group when compared with the EUE group and the CE group(P<0.05).Conclusions The decreased expression of TWEAK maybe promote the endometrial cell proliferation and metastasis.The increased expression of Fn14 can be compensated to reduce the expression of TWEAK.The abnormal expression indicated TWEAK and Fn14 may be involved in the occurrence and development of endometriosis.
引文
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[11]Kwon O,Park SJ,Kang TW,et al.Elevated fibroblast growth factor-inducible 14 expression promotes gastric cancer growth via nuclear factor-κB and is associated with poor patient outcome[J].Cancer Lett.2012,314(1):73-81.
[2]Lynch CN,Wang YC,Lund J K,et al.TWEA K induces angiogenesis and proliferation of endothelial cells.J Biol Chem 1999,274,8455-8459.
[3]Dai L,Gu L,Ding C,et a1.TWEAK promotes ovarian cancer cell metastasis NF--kappaB pathway activation and VEGF expression[J].Cancer lett,2009,283(2):159-167.
[4]Reis MF,Petraglia F,Taylor RN,et al.Endometriosis:hormone regulation and clinical consequences of chemotaxis and apoptosis[J].Hum Reprod Update,2013,19:406-418.
[5]Bouquet DJJ,Ayoubi JM,Gianaroli L.Endometriosis::a new cellular and molecular genetic approach for understanding the pathogenesis and evolutivity[J].Front Surg,2014,27:1:16.
[6]Wang X,Zhao X,Wang K,et al.Interaction of monocytes/macrophages with ovarian cancer cells promotes angiogenesis in vitro.CancerSci,2013,104(4):516-523.
[7]Winkles JA,Tran NL,Bmwn sA,et al.Role of TWEAK and Fnl4in tumor biology[J].Front Biosci,2007,12:2761-2771.
[8]Winkles J A,Tran N L,Brown S A,et al.Role of TWEAK and Fn14 in tumor biology[J].Front Biosci,2007,12:2761-2771.
[9]Schneider P,Schwenzer R,Haas E,et al.TWEAK can induce cell death via endogenous TNF and TNF receptorⅠ[J].Eur J Immunol,1999,29(6):1785-1792.
[10]Nakayama M,Ishidoh K,Kayagaki N,et al.Multiple pathways of TWEAK-induced cell death[J].J Immunol,2002,168(2):734-743.
[11]Kwon O,Park SJ,Kang TW,et al.Elevated fibroblast growth factor-inducible 14 expression promotes gastric cancer growth via nuclear factor-κB and is associated with poor patient outcome[J].Cancer Lett.2012,314(1):73-81.