多指标正交试验优化复方红景天颗粒的提取工艺
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摘要
目的:优化复方红景天颗粒的药材提取工艺。方法:以药效学指标(学习记忆能力)对小鼠进行Y型水迷宫实验,筛选复方红景天颗粒药材的水提、水提醇沉及醇提方法;以红景天苷、阿魏酸提取量及出膏率的综合评分为指标,采用正交试验优化醇提工艺中乙醇体积分数、提取时间、溶剂用量和提取次数,并进行验证试验。结果:3种提取方法中以醇提方法所得产物对改善学习记忆能力效果最好;最优醇提工艺为8倍量70%乙醇回流提取3次,每次1.0 h。验证试验中红景天苷、阿魏酸的提取量及出膏率平均值分别为4.884 mg/g、1.669 mg/g、33.52%(RSD均小于2%,n=3)。结论:优化的提取工艺稳定、可靠、简便、易行,可用于复方红景天颗粒的工业化提取。
OBJECTIVE:To optimize the extraction technology of Compound Rhodiolae crenulatae granule. METHODS:The pharmacodynamic index of learning and memory ability was used to screen the water extraction,water extraction and alcohol precipitation and alcohol extraction methods for Compound R. crenulatae granule in mice by Y-type water maze test. Using the comprehensive scores of salidroside,ferulic acid extraction amount and extraction yield as indexes,orthogonal test was used to optimize the ethanol volume fraction,extraction time,solvent amount and extraction times,and verification test was conducted. RESULTS:Products by alcohol extraction can significantly improve learning and memory ability in the 3 methods;the optimal extraction tech-nology was confirmed to 8-fold 70% ethanol reflux extracting for 3 times,1.0 h each time. In verification test,average extraction amount of salidroside and ferulic acid were 4.884 mg/g and 1.669 mg/g,and average extraction yield was 33.52%(all RSD<2%,n=3). CONCLUSIONS:The optimized extraction technology is stable,reliable,simple and feasible,which can be used for industrial extraction of Compound R. crenulatae granule.
OBJECTIVE:To optimize the extraction technology of Compound Rhodiolae crenulatae granule. METHODS:The pharmacodynamic index of learning and memory ability was used to screen the water extraction,water extraction and alcohol precipitation and alcohol extraction methods for Compound R. crenulatae granule in mice by Y-type water maze test. Using the comprehensive scores of salidroside,ferulic acid extraction amount and extraction yield as indexes,orthogonal test was used to optimize the ethanol volume fraction,extraction time,solvent amount and extraction times,and verification test was conducted. RESULTS:Products by alcohol extraction can significantly improve learning and memory ability in the 3 methods;the optimal extraction tech-nology was confirmed to 8-fold 70% ethanol reflux extracting for 3 times,1.0 h each time. In verification test,average extraction amount of salidroside and ferulic acid were 4.884 mg/g and 1.669 mg/g,and average extraction yield was 33.52%(all RSD<2%,n=3). CONCLUSIONS:The optimized extraction technology is stable,reliable,simple and feasible,which can be used for industrial extraction of Compound R. crenulatae granule.
引文
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[3]何蕾,马慧萍,李兰茹,等.小鼠抗缺氧活性实验结合正交试验优选藏紫菀中总黄酮的提取工艺[J].中国药房,2016,27(13):1832-1835.
[4]顾亦婷,顾海.阿兹海默症中西医辨证治疗[J].辽宁中医药大学学报,2015,17(8):139-141.
[5]张佳,宋立刚,孔卫娜,等.红景天苷对Aβ1~40所致阿尔茨海默病模型大鼠认知功能改善作用及机制探讨[J].中国中药杂志,2012,37(14):2122-2126.
[6]杨长生,杨植媛,吴红彦,等.阿魏酸对Aβ25~35诱导PC12细胞的保护机制研究[J].辽宁中医杂志,2015,42(1):33-35.
[7]任爱农,卢爱玲,田耀洲,等.层次分析法用于中药复方提取工艺的多指标权重研究[J].中国中药杂志,2008,33(4):372-374.
[8]李仪奎.中药药理实验方法学[M].上海:科学技术出版社,1991:172-175.
[9]皮慧芳,阮汉利,张鹏,等.黄花石蒜对老年痴呆小鼠的药效学研究[J].中华中医药学刊,2007,25(10):2049-2050.
[10]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:41、155.
[11]刘桂花,何承辉,帕依曼·亥米提,等.多指标综合评分法优选复方双金感冒颗粒的提取工艺[J].中国药房,2016,27(4):510-513.
[12]张瑞堂,石晓峰,张红梅.复方红景天口含片提取工艺优选[J].中国实验方剂学杂志,2011,17(13):42-45.