活性氧调控因子1在人退变椎间盘中的表达及意义
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摘要
目的探讨活性氧调控因子1(reactive oxygen species modulator 1,ROMO1)在退变椎间盘髓核中的表达。方法采用免疫组化和荧光探针DCFH-DA检测不同退变髓核组织中ROMO1的表达与(reactive oxygen species,ROS)含量,并分析ROMO1表达水平与ROS含量、椎间盘退变程度的相关性。结果各组ROMO1的平均光密度值依次为0.192±0.089、0.328±0.048、0.399±0.053、0.468±0.098;各组ROS结果分别为132.961±15.149、191.889±17.880、218.056±12.845、243.501±30.279。随着椎间盘退变程度的加重,ROMO1蛋白表达量与ROS含量逐渐升高,且差异具有显著性(P<0.05)。ROMO1的表达水平与ROS的含量及椎间盘退变程度均呈正相关关系(P<0.05)。结论 ROMO1的表达水平与椎间盘的退变程度呈正相关,这可能与其增加髓核组织内ROS含量而加重氧化应激损伤有关。
Objective To explore the expression and their relationship of ROMO1 and ROS in the degenerative intervertebral disc. Methods Immunohistochemical techniques and fluorescent probes DCFHDA were applied to detect the expression of ROMO1 and ROS levels in the different degenerated nucleus pulposus. Results The mean option density of ROMO1 were 0.192±0.089, 0.328±0.048, 0.399±0.053, and0.468 ± 0.098, respectively. The results of ROS detection were 132.961 ± 15.149, 191.889 ± 17.880, 218.056 ±12.845, and 243.501 ± 30.279,separately,in the groups of A, B, C, and D. It showed the expression of ROMO1 and the content of ROS increased gradually with the aggravation of intervertebral disc degeneration,and there was a positive correlation between ROMO1 expression level and the content of ROS in intervertebral disc(r=0.732, P<0.05). There was also a positive correlation between ROMO1 expression level and the intervertebral disc degeneration degree(r=0.603, P<0.05). Conclusion ROMO1 and ROS are highly expressed in the nucleus pulposus tissue of the degenerated intervertebral disc, which has a positive correlation; and the expression level of ROMO1 is positively correlated with the degree of degeneration of intervertebral disc, which might be related to aggravated oxidative stress injury by increasing the content of ROS in the nucleus pulposus.
Objective To explore the expression and their relationship of ROMO1 and ROS in the degenerative intervertebral disc. Methods Immunohistochemical techniques and fluorescent probes DCFHDA were applied to detect the expression of ROMO1 and ROS levels in the different degenerated nucleus pulposus. Results The mean option density of ROMO1 were 0.192±0.089, 0.328±0.048, 0.399±0.053, and0.468 ± 0.098, respectively. The results of ROS detection were 132.961 ± 15.149, 191.889 ± 17.880, 218.056 ±12.845, and 243.501 ± 30.279,separately,in the groups of A, B, C, and D. It showed the expression of ROMO1 and the content of ROS increased gradually with the aggravation of intervertebral disc degeneration,and there was a positive correlation between ROMO1 expression level and the content of ROS in intervertebral disc(r=0.732, P<0.05). There was also a positive correlation between ROMO1 expression level and the intervertebral disc degeneration degree(r=0.603, P<0.05). Conclusion ROMO1 and ROS are highly expressed in the nucleus pulposus tissue of the degenerated intervertebral disc, which has a positive correlation; and the expression level of ROMO1 is positively correlated with the degree of degeneration of intervertebral disc, which might be related to aggravated oxidative stress injury by increasing the content of ROS in the nucleus pulposus.
引文
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[2]Rizzo JA,Abbott TA,3rd,Berger ML.The labor productivity effects of chronic backache in the United States[J].Med Care,1998,36(10):1471-1488.
[3]Schwarzer AC,Aprill CN,Derby R,et al.The prevalence and clinical features of internal disc disruption in patients with chronic low back pain[J].Spine,1995,20(17):1878-1883.
[4]Zhang YG,Sun Z,Zhang Z,et al.Risk factors for lumbar intervertebral disc herniation in Chinese population:a case-control study[J].Spine,2009,34(25):E918-922.
[5]Ito H,Kurokawa H,Hirayama A,et al.Cancer cell-specific mitochondrial reactive oxygen species promote non-heme iron uptake and enhance the proliferation of gastric epithelial cancer cell[J].J Clin Biochem Nutr,2017,61(3):183-188.
[6]Warnatsch A,Tsourouktsoglou TD,Branzk N,et al.Reactive oxygen species localization programs inflammation to clear microbes of different size[J].Immunity,2017,46(3):421-432.
[7]Nasto LA,Robinson AR,Ngo K,et al.Mitochondrial-derived reactive oxygen species(ROS)play a causal role in aging-related intervertebral disc degeneration[J].J Orthop Res,2013,31(7):1150-1157.
[8]谢荣辉,殷明,殷嫦嫦,等.绿原酸保护大鼠髓核细胞对抗氧化应激的作用机制研究[J].中药材,2014,37(4):465-469.
[9]Klawitter M,Quero L,Klasen J,et al.Triptolide exhibits antiinflammatory,anti-catabolic as well as anabolic effects and suppresses TLR expression and MAPK activity in IL-1beta treated human intervertebral disc cells[J].Eur Spine J,2012,21(Suppl 6):850-859.
[10]Yang L,Rong Z,Zeng M,et al.Pyrroloquinoline quinone protects nucleus pulposus cells from hydrogen peroxide-induced apoptosis by inhibiting the mitochondria-mediated pathway[J].Eur Spine J,2015,24(8):1702-1710.
[11]Yang X,Jin L,Yao L,et al.Antioxidative nanofullerol prevents intervertebral disk degeneration[J].Int J Nanomedicine,2014,9(24):2419-2430.
[12]Lee SB,Kim JJ,Kim TW,et al.Serum deprivation-induced reactive oxygen species production is mediated by Romo1[J].Apoptosis,2010,15(2):204-218.
[13]Chung YM,Lee SB,Kim HJ,et al.Replicative senescence induced by Romo1-derived reactive oxygen species[J].J Biol Chem,2008,283(48):33763-33771.
[14]Hwang IT,Chung YM,Kim JJ,et al.Drug resistance to 5-FU linked to reactive oxygen species modulator 1[J].Biochem Biophys Res Commun,2007,359(2):304-310.
[15]Park JB,Byun CH,Park EY.Rat notochordal cells undergo premature stress-induced senescence by high glucose[J].Asian Spine J,2015,9(4):495-502.
[16]Shin JA,Chung JS,Cho SH,et al.Romo1 expression contributes to oxidative stress-induced death of lung epithelial cells[J].Biochem Biophys Res Commun,2013,439(2):315-320.
[17]Lee SH,Min JW,Lee JS,et al.Reactive oxygen species modulator 1(Romo1)overexpression is an independent predictor of poor survival in NSCLC patients who undergo surgical resection[J].Lung Cancer,2015,87(1):45-52.
[18]Lee S,Park YH,Chung JS,et al.Romo1 and the NF-κB pathway are involved in oxidative stress-induced tumor cell invasion[J].Int J Oncol,2015,46(5):2021-2028.
[19]Chung JS,Lee S,Yoo YD.Constitutive NF-κB activation and tumorgrowth promotion by Romo1-mediated reactive oxygen species production[J].Biochem Biophys Res Commun,2014,450(4):1656-1661.
[20]Shen J,Fang J,Hao J,et al.SIRT1 inhibits the catabolic effect of IL-1beta through TLR2/SIRT1/NF-κB pathway in human degenerative nucleus pulposus cells[J].Pain Physician,2016,19(1):E215-226.
[21]Zhongyi S,Sai Z,Chao L,et al.Effects of nuclear factor kappa B signaling pathway in human intervertebral disc degeneration[J].Spine,2015,40(4):224-232.