不同抗旱类型胡麻幼苗对干旱胁迫的生理响应
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摘要
旨为阐明不同抗旱类型胡麻对干旱胁迫的生理响应规律,以抗旱性由强到弱4个不同抗旱类型的胡麻品种晋亚7号、晋亚10号、晋亚11号、E051-20为材料,采用盆栽控水法,研究了干旱胁迫对不同抗性胡麻生长、膜质过氧化及渗透调节物质含量及过氧化物酶活性的影响。结果表明,随着干旱胁迫程度的加深及时间的延长,幼苗的株高、茎粗及植株的生物量和叶片相对含水量均显著降低;抗旱性强的品种下降幅度明显低于抗旱性弱的品种,依次为晋亚7号<晋亚10号<晋亚11号晋亚10号>晋亚11号>E051-20。抗性弱的品种MDA和质膜透性的增幅明显高于抗旱性强的品种,可溶性蛋白含量相对稳定。POD酶活性总体呈现上升的趋势,抗旱性强的品种上升幅度明显高于抗性弱的品种。相关性分析结果表明,不同品系胡麻的株高、茎粗与生物量间均呈极显著正相关,与MDA、Pro含量间均呈极显著负相关。因此,可以用Pro、MDA和生物量作为鉴定胡麻幼苗抗旱性的生理指标。
Aiming to elucidate the physiological response mechanism of different drought resistant flax strains to drought stress. In a pot culture, four flax varieties(Jinya 7, Jinya 10, Jinya 11 and E051-20) with different types of drought resistance were used as the materials to study the effects of drought stress on the growth, membrane lipid peroxidation and osmotic adjustment substance content of flax seedlings. The results showed that, with the increase of drought stress and the length of stress time, the plant height, stem diameter, biomass and leaf relative water content of the plant decreased significantly, and the strong drought-resistant varieties decreased significantly less than the weak drought-resistant varieties, which was in turn: Jinya 7 Jinya 10 > Jinya 11 > E051-20. The malondialdehyde and plasma membrane permeability in weak resistant varieties increased more than in strong resistant varieties, and the amount of increase in turn was Jinya 7
Aiming to elucidate the physiological response mechanism of different drought resistant flax strains to drought stress. In a pot culture, four flax varieties(Jinya 7, Jinya 10, Jinya 11 and E051-20) with different types of drought resistance were used as the materials to study the effects of drought stress on the growth, membrane lipid peroxidation and osmotic adjustment substance content of flax seedlings. The results showed that, with the increase of drought stress and the length of stress time, the plant height, stem diameter, biomass and leaf relative water content of the plant decreased significantly, and the strong drought-resistant varieties decreased significantly less than the weak drought-resistant varieties, which was in turn: Jinya 7
引文
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[2] 陈善福,舒庆尧.植物耐干旱胁迫的生物学机理及其基因工程研究进展[J].植物学通报,1999,16(5):555-560.Chen S F,Shu Q Y.Biological mechanism of and genetic engineering for drought stress tolerance in plants[J].Chinese Bulletin of Botany,1999,16(5):555-560.
[3] Gauch H G,Zobel R W.AMMI analysis of yield trials[M]// Kang M S,Gauch H G,eds.Genotype-by-environment Interaction.Boca Raton:CRC Press,1996:85-122.
[4] 郭娜,李爱荣,马建富,刘栋.施磷水平对胡麻干物质积累与产量的影响[J].河北农业科学,2015,19(1):14-17.doi:10.16318/j.cnki.hbnykx.2015.01.004.Guo N,Li A R,Ma J F,Liu D.Effects of different phosphorus application levels on dry matter accumulation and yield of flax[J].Journal of Hebei Agricultural Sciences,2015,19(1):14-17.
[5] 祁旭升,王兴荣,许军,张建平,米君.胡麻种质资源成株期抗旱性评价[J].中国农业科学,2010,43(15):3076-3087.doi:10.3864/j.issn.0578-1752.2010.15.004.Qi X S,Wang X G,Xu J,Zhang J P,Mi J.Drought-resistance evaluation of flax germplasm at adult plant stage[J].Scientia Agricultura Sinica,2010,43(15):3076-3087.
[6] 党占海,赵蓉英,王敏,党照.国际视野下胡麻研究的可视化分析[J].中国麻业科学,2010,32(6):305-313.Dang Z H,Zhao R Y,Wang M,Dang Z.Visualization analysis on oil flax research-based on the view of internationalization[J].Plant Fiber Sciences in China,2010,32(6):305-313.
[7] 党占海,张建平.我国亚麻产业现状及发展对策[M].北京:中国农业科学技术出版社,2004.Dang Z H,Zhang J P.Present situation and development countermeasures of flax industry in China[J].Beijing:China Agriculture Science and Technology Press,2004.
[8] 张正斌.作物抗旱节水的生理遗传育种基础[M].北京:科学出版社,2003:67-209.Zhang Z B.Physiological genetic breeding basis for drought-resistant and water-saving crops[J].Beijing:Science Press,2003:67-209.
[9] 姚宁,宋利兵,刘健,冯浩,吴淑芳,何建强.不同生长阶段水分胁迫对旱区冬小麦生长发育和产量的影响[J].中国农业科学,2015,48(12):2379-2389.doi:10.3864/j.issn.0578-1752.2015.12.011.Yao N,Song L B,Liu J,Feng H,Wu S F,He J Q.Effects of water stress at different growth stages on the development and yields of winter wheat in arid region[J].Scientia Agricultura Sinica,2015,48(12):2379-2389.
[10] 鞠乐,齐军仓,成禄艳,赵佳,廖永兵.大麦种子萌发期对干旱胁迫的生理响应及其抗旱性评价[J].西南农业学报,2013,26(1):93-98.doi:10.16213/j.cnki.scjas.2013.01.040.Ju L,Qi J C,Cheng L Y,Zhao J,Liao Y B.Physiological response of barley to drought stress during seed germination period and drought resistance evaluation[J].Southwest China Journal of Agricultural Sciences,2013,26(1):93-98.
[11] 姚启伦,陈秘.干旱胁迫对玉米地方品种苗期植株形态的影响[J].河南农业科学,2010(2):20-27.doi:10.15933/j.cnki.1004-3268.2010.02.016.Yao Q L,Chen M.The effects of drought s tress on plant morphologyof maize landraces at seedling stage[J].Journal of Henan Agricultural Sciences,2010(2):20-27.
[12] 王燕平,王晓梅,侯国强,孙晓环,齐主鑫,宗春美,白艳凤,徐德海,郭数进,李贵全,任海祥.室内模拟干旱对大豆萌发性状的影响及抗旱性评价[J].核农学报,2014,28(6):1133-1141.Wang Y P,Wang X M,Hou G Q,Sun X H,Qi Z X,Zong C M,Bai Y F,Xu D H,Guo S J,Li G Q,Ren H X.Effects of indoor simulated drought stress on germinal traits ofsoybeans and evaluation of drought-resistant[J].Journal of Nuclear Agricultural Sciences,2014,28(6):1133-1141.
[13] 于惠琳,史振声,丛玲,朱振兴.干旱胁迫下甜高粱和粒用高粱光合及生理响应比较[J].江苏农业科学,2014,42(2):72-75.doi:10.15889/j.issn.1002-1302.2014.02.169.Yu H L,Shi Z S,Cong L,Zhu Z X.The comparison of photosynthesis and physiological response of sorghum and grain sorghum under drought stress[J].Jiangsu Agricultural Sciences,2014,42(2):72-75.
[14] 刘长海,周莎莎,邹养军,梁东,马锋旺.干旱胁迫条件下不同抗旱性苹果砧木内源激素含量的变化[J].干旱地区农业研究,2012,30(5):94-98.Liu C H,Zhou S S,Zou Y J,Liang D,Ma F W.Changes of endogenous hormones contents of two different drought-tolerant Malus rootstocks in response to drought stress[J].Agricultural Research in the Arid Areas,2012,30(5):94-98.
[15] 郑国琦,谢亚军.干旱胁迫对宁夏枸杞幼苗膜脂过氧化及抗氧化保护酶活性的影响[J].安徽农业科学,2008,36(4):1343-1344,1552.doi:10.13989/j.cnki.0517-66 11.2008.04.083.Zheng G Q,Xie Y J.Effects of drought stress on the changes of membrane-lipid peroxidation and antioxidase activity in the seedlings of Lycium barbarum L.[J].Journal of Anhui Agricultural Sciences,2008,36(4):1343-1344,1552.
[16] 梁新华,史大刚.干旱胁迫对光果甘草幼苗根系MDA含量及保护酶POD,CAT活性的影响[J].干旱地区农业研究,2006,24(3):108-110.Liang X H,Shi D G.Effect of drought stress on the content of malondialdehyole and activity of cell defense enzymes in Glycyrrhiza glabra seedlings roots[J].Agricultural Research in the Arid Areas,2006,24(3):108-110.
[17] 王玉富,粟建光.亚麻种质资源描述规范和数据标准[M].北京:中国农业出版社,2006:1-99.Wang Y F,Li J G.Specification and data standard for flax germplasm resources[M].Beijing:China Agriculture Press,2006:1-99.
[18] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:258-260.Li H S.The experiment principle and technique on plant physiology and biochemistry[M].Beijing:Higher Education Press,2000:258-260.
[19] 王孝平,刑树礼.考马斯亮蓝法测定蛋白质含量的研究[J].天津化工,2009,23(3):88-90.Wang X P,Xing S L.Determination of protein content by coomassie brilliant blue method[J].Tianjin Chemical Industry,2009,23(3):88-90.
[20] Madhava R K V,Sresty T V S.Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan L.Millspaugh) in response to Zn and Ni stresses[J].Plant Science,2000,157:113-128.
[21] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:211-221.Gao J F.Plant physiology experiment guidance[M].Beijing:Higher Education Press,2006:211-221.
[22] Stewart C R.Mctabolism of proline by leaves and its use in means during the effect of water stress on proline oxidation[J].Plant Physiology,1978,61:654-657.
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