45℃艾灸对高脂血症大鼠血脂及血清ox-LDL、NO的影响
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摘要
目的:观察不同温度(38℃、45℃)艾灸对高脂血症大鼠血脂及血清氧化性低密度脂蛋白(ox-LDL)、一氧化氮(NO)的影响,探讨45℃艾灸调脂效应及抗氧化应激、保护血管内皮的相关性。方法:将60只SD大鼠按照随机数字表法随机分为正常组、模型组、38℃艾灸组和45℃艾灸组,每组15只。正常组不予处理;其余3组用高脂饲料连续喂养8周,造高脂血症大鼠模型。造模成功后,模型组不予处理,38℃艾灸组和45℃艾灸组均取"神阙"和"足三里"穴,给予不同温度艾灸治疗,温度分别控制在(38±1)℃、(45±1)℃,每次每穴艾灸10 min,隔日1次,治疗4周。治疗结束取血清,采用生化比色法测定血清胆固醇(TC)、三酯甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C),采用酶联免疫吸附法(ELISA)检测血清ox-LDL、NO水平。结果:(1)模型组与正常组比较,TC、TG、LDL-C升高(均P<0.01);45℃艾灸组与模型组、38℃艾灸组比较,TC、TG、LDL-C降低(P<0.01,P<0.05);38℃艾灸组与模型组比较,TC、TG、LDL-C降低,但差异无统计学意义(均P>0.05)。(2)模型组与正常组比较,ox-LDL增高、NO降低(均P<0.01);45℃艾灸组与模型组、38℃艾灸组比较,ox-LDL降低,NO升高(P<0.01,P<0.05);38℃艾灸组与模型组比较,ox-LDL降低,NO升高(均P<0.05)。结论:45℃艾灸对高脂血症大鼠有调脂作用,可通过抗氧化保护血管内皮多途径调节血脂。
Objective To observe the effects of moxibustion at different temperatures(38 ℃ and 45 ℃) on blood lipoids and serum level of oxidized low density lipoprotein(ox-LDL) and nitric oxide(NO) in rats with hyperlipidemia, and to explore the correlation between regulating blood fat and anti-oxidative stress and protection of vascular endothelium of moxibustion at45 ℃. Methods According to random number table, 60 SD rats were randomly divided into a normal group, a model group, a moxibustion at 38 ℃ group and a moxibustion at 45 ℃ group, 15 rats in each group. The rats in the normal group received no treatment; the rats in the remaining three groups were fed with high-fat diet for 8 weeks to prepare rat models of hyperlipidemia. After successful modeling, the rats in the model group received no treatment; the rats in the moxibustion at38 ℃ group and moxibustion at 45 ℃ group were treated with moxibustion at "Shenque"(CV 8) and "Zusanli"(ST 36), and the temperature was controlled at(38±1) ℃ and(45±1) ℃, respectively. The moxibustion was given for 10 min at each acupoint, once every two days, and totally 4-week treatment was given. After treatment, the levels of total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C) were measured by using biochemical colorimetric method; the levels of ox-LDL and NO were measured by using ELISA method. Results(1) Compared with the normal group, the levels of TC, TG and LDL-C were significantly increased in the model group(all P<0.01);compared with the model group and moxibustion at 38 ℃ group, the levels of TC, TG and LDL-C were significantly decreased in the moxibustion at 45 ℃ group(P<0.01,P<0.05); compared with the model group, the levels of TC, TG and LDL-C were insignificantly decreased in the moxibustion at 38 ℃ group(all P>0.05).(2) Compared with the normal group, the level of ox-LDL was increased but that of NO was decreased in the model group(both P<0.01); compared with the model group and moxibustion at38 ℃ group, the level of ox-LDL was decreased but that of NO was increased in the moxibustion at 45 ℃ group(P<0.01, P<0.05);compared with the model group, the level of ox-LDL was decreased but that of NO was increased in the moxibustion at 38 ℃ group(both P<0.05). Conclusion Moxibustion at 45 ℃ has regulating effects on blood lipid in rats with hyperlipidemia, which can regulate blood lipid through various ways, such as anti-oxidative stress and protection of vascular endothelium.
Objective To observe the effects of moxibustion at different temperatures(38 ℃ and 45 ℃) on blood lipoids and serum level of oxidized low density lipoprotein(ox-LDL) and nitric oxide(NO) in rats with hyperlipidemia, and to explore the correlation between regulating blood fat and anti-oxidative stress and protection of vascular endothelium of moxibustion at45 ℃. Methods According to random number table, 60 SD rats were randomly divided into a normal group, a model group, a moxibustion at 38 ℃ group and a moxibustion at 45 ℃ group, 15 rats in each group. The rats in the normal group received no treatment; the rats in the remaining three groups were fed with high-fat diet for 8 weeks to prepare rat models of hyperlipidemia. After successful modeling, the rats in the model group received no treatment; the rats in the moxibustion at38 ℃ group and moxibustion at 45 ℃ group were treated with moxibustion at "Shenque"(CV 8) and "Zusanli"(ST 36), and the temperature was controlled at(38±1) ℃ and(45±1) ℃, respectively. The moxibustion was given for 10 min at each acupoint, once every two days, and totally 4-week treatment was given. After treatment, the levels of total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C) were measured by using biochemical colorimetric method; the levels of ox-LDL and NO were measured by using ELISA method. Results(1) Compared with the normal group, the levels of TC, TG and LDL-C were significantly increased in the model group(all P<0.01);compared with the model group and moxibustion at 38 ℃ group, the levels of TC, TG and LDL-C were significantly decreased in the moxibustion at 45 ℃ group(P<0.01,P<0.05); compared with the model group, the levels of TC, TG and LDL-C were insignificantly decreased in the moxibustion at 38 ℃ group(all P>0.05).(2) Compared with the normal group, the level of ox-LDL was increased but that of NO was decreased in the model group(both P<0.01); compared with the model group and moxibustion at38 ℃ group, the level of ox-LDL was decreased but that of NO was increased in the moxibustion at 45 ℃ group(P<0.01, P<0.05);compared with the model group, the level of ox-LDL was decreased but that of NO was increased in the moxibustion at 38 ℃ group(both P<0.05). Conclusion Moxibustion at 45 ℃ has regulating effects on blood lipid in rats with hyperlipidemia, which can regulate blood lipid through various ways, such as anti-oxidative stress and protection of vascular endothelium.
引文
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[7]马明云,姜劲峰,周小云,等.艾灸对高脂血症患者血脂、血糖的影响[J].上海针灸杂志,2012,31(8):535-536.
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[12]陈若暘,高建芸,苏方方,等.神阙灸法治疗EAT模型小鼠的疗效及机制初探[J].东南大学学报(医学版),2017,36(5):752-757.
[13]李春晖.氧化应激与动脉粥样硬化研究进展[J].中华实用中西医杂志,2008,21(14):1178-1180.
[14]Sakurai K,Sawamura T.Endothelial dysfunction via lectin-like oxidized low-density lipoprotein receptor-1:Close relationships with oxidative stress[J].2003,91(3):182-186.
[15]de Oliveira J,Hort MA,Moreira EL,et al.Positive correlation between elevated plasma cholesterol levels and cognitive impairments in LDL receptor knockout mice:relevance of cortico-cerebral mitochondrial dysfunction and oxidative stress[J].Neuroscience,2011,197(197):99-106.
[16]葛进,郑喜兰,雒2),等.替米沙坦对高脂喂养大鼠血清NO及i NOS的影响[J].天津医药,2011,39(6):561-562.
[17]嵇明月,王玲玲,姜劲峰.温和灸对高脂血症大鼠氧化应激及炎症反应的影响[J].中华中医药杂志,2013,28(8):2301-2304.
[18]张会芳,王玲玲,嵇明月,等.温和灸对高脂血症大鼠血脂及血清NO的影[J].中国针灸,2013,33(5):438-442.
[19]Yang D,Luo Z,Ma S,et al.Activation of TRPV1 by dietary capsaicin improves endothelium-dependent vasorelaxation and prevents hypertension[J].Cell Metabolism,2010,12(2):130-141.
[20]吴以岭.“脉络-血管系统”相关性探讨[J].中医杂志,2007,48(1):5-8.
[2]Tsimikas S,Bergmark C,Beyer RW,Patel R,et al.Temporal increases in plasma markers of oxidized low-density lipoprotein strongly reflect the presence of acute coronary syndromes[J].J Am Col Cardiol,2003,41(3):360-370.
[3]Bian K,Ke Y,Kamisaki Y,et al.Proteomic modification by nitric oxide.[J].J Pharmacol Sci,2006,101(4):271.
[4]尹仁,吴焕淦,李璟,等.艾灸法治疗高脂血症并动脉粥样硬化研究进展[J].中国中医药信息杂志,2011,18(11):104-107.
[5]高建芸,王桂英,王玲玲.TRPV1介导不同温度艾灸调脂通脉效应的临床研究[J].时珍国医国药,2015,26(9):2182-2184.
[6]陈仲杰,吴中朝,李彩芬,等.不同艾灸时程对高脂血症调脂效应影响的研究[J].中国针灸,2012,32(11):995-999.
[7]马明云,姜劲峰,周小云,等.艾灸对高脂血症患者血脂、血糖的影响[J].上海针灸杂志,2012,31(8):535-536.
[8]Wang GY,Wang LL,Xu B,et al.Effects of moxibustion temperature on blood cholesterol level in a mice model of acute hyperlipidemia:role of TRPV1[J].Evid Based Complement Alternat Med,2013,2013(11):871704.
[9]王根辈,栗志文,曹晶,等.高脂饮食诱发大鼠营养性肥胖动物模型的研究[J].吉林医学,2012,33(1):5-7.
[10]Goel R,Boylan B,Gruman L,et al.The proinflammatory phenotype of PECAM-1-deficient mice results in atherogenic diet-induced steatohepatitis[J].Am J Physiol Gastrointest Liver Physiol,2007,293(6):G1205.
[11]Wang YS,Zhang JB,Jiang JF,et al.Research on effects of the thermal stimulation by moxibustion at different temperatures on cardiac function in rats and on mast cells in the local site of moxibustion[J].Evidence Based Complement Alternat Med,2013,2013:545707.
[12]陈若暘,高建芸,苏方方,等.神阙灸法治疗EAT模型小鼠的疗效及机制初探[J].东南大学学报(医学版),2017,36(5):752-757.
[13]李春晖.氧化应激与动脉粥样硬化研究进展[J].中华实用中西医杂志,2008,21(14):1178-1180.
[14]Sakurai K,Sawamura T.Endothelial dysfunction via lectin-like oxidized low-density lipoprotein receptor-1:Close relationships with oxidative stress[J].2003,91(3):182-186.
[15]de Oliveira J,Hort MA,Moreira EL,et al.Positive correlation between elevated plasma cholesterol levels and cognitive impairments in LDL receptor knockout mice:relevance of cortico-cerebral mitochondrial dysfunction and oxidative stress[J].Neuroscience,2011,197(197):99-106.
[16]葛进,郑喜兰,雒2),等.替米沙坦对高脂喂养大鼠血清NO及i NOS的影响[J].天津医药,2011,39(6):561-562.
[17]嵇明月,王玲玲,姜劲峰.温和灸对高脂血症大鼠氧化应激及炎症反应的影响[J].中华中医药杂志,2013,28(8):2301-2304.
[18]张会芳,王玲玲,嵇明月,等.温和灸对高脂血症大鼠血脂及血清NO的影[J].中国针灸,2013,33(5):438-442.
[19]Yang D,Luo Z,Ma S,et al.Activation of TRPV1 by dietary capsaicin improves endothelium-dependent vasorelaxation and prevents hypertension[J].Cell Metabolism,2010,12(2):130-141.
[20]吴以岭.“脉络-血管系统”相关性探讨[J].中医杂志,2007,48(1):5-8.