口腔扁平苔藓患者血清中神经生长因子和P物质的表达及意义
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摘要
目的:探讨口腔扁平苔藓(OLP)患者血清中神经生长因子(NGF)和P物质(SP)的表达及意义。方法:流式细胞术及散射比浊法分析30例OLP患者和20例健康对照组外周血淋巴细胞亚群(CD3+、 CD4+、 CD8+、 CD19+、 CD16+56+)和体液免疫指标(IgG、 IgA、 IgM、 C3、 C4)的表达。酶联免疫吸附试验(ELISA)检测OLP患者血清NGF和SP的表达并分析其与OLP免疫功能的相关性。结果:OLP患者CD3+、 CD4+、 CD8+、 C3、 C4低于正常值(P<0.05),CD19+、CD4+/CD8+及IgM高于正常值(P<0.05)。OLP组的NGF和SP均高于对照组(P<0.05),糜烂型OLP高于非糜烂型(P<0.05)。OLP组NGF和SP表达呈正相关且二者均与OLP体征计分正相关(P<0.05),与CD19+、 IgA含量呈正相关(P<0.05)。结论:NGF和SP的高表达可能在OLP中参与了神经系统对免疫应答的调节作用,二者共同参与了OLP炎症发展。
Objective: To investigate the expression and significance of nerve growth factor(NGF) and substance P(SP) in serum of patients with oral lichen planus(OLP). Methods: The peripheral blood lymphocyte subsets(CD3+, CD4+, CD8+, CD19+, CD16+56+) and humoral immunity(IgG, IgA, IgM, C3, C4) in 30 patients with OLP were detected by flow cytometry and nephelometry assays respectively, NGF and SP contents in serum were detected by ELISA. The correlations between the levels of NGF, SP, immune status and clinical characteristics of OLP patients were analyzed respectively. 20 healthy subjects were served as the controls. Results: CD3+, CD4+, CD8+, C3 and C4 in OLP patients were lower than the normal values(P<0.05), whereas the expression of CD19+, CD4+/CD8+ and IgM was higher than the normal values(P<0.05). NGF and SP levels showed significant upregulation in OLP patients compared with control group(P<0.05), and in the OLP patients of erosive type was higher than those of non-erosive type(P<0.05). Meanwhile, positive correlations existed between NGF and SP in the OLP serum(P<0.05), both of them were positively correlated with OLP clinical characteristics(P<0.05), CD19+(P<0.05) and IgA(P<0.05), respectively. Conclusion: Over expression of NGF and SP may be involved in the communication of nervous-immune system of OLP and promote the development of OLP.
Objective: To investigate the expression and significance of nerve growth factor(NGF) and substance P(SP) in serum of patients with oral lichen planus(OLP). Methods: The peripheral blood lymphocyte subsets(CD3+, CD4+, CD8+, CD19+, CD16+56+) and humoral immunity(IgG, IgA, IgM, C3, C4) in 30 patients with OLP were detected by flow cytometry and nephelometry assays respectively, NGF and SP contents in serum were detected by ELISA. The correlations between the levels of NGF, SP, immune status and clinical characteristics of OLP patients were analyzed respectively. 20 healthy subjects were served as the controls. Results: CD3+, CD4+, CD8+, C3 and C4 in OLP patients were lower than the normal values(P<0.05), whereas the expression of CD19+, CD4+/CD8+ and IgM was higher than the normal values(P<0.05). NGF and SP levels showed significant upregulation in OLP patients compared with control group(P<0.05), and in the OLP patients of erosive type was higher than those of non-erosive type(P<0.05). Meanwhile, positive correlations existed between NGF and SP in the OLP serum(P<0.05), both of them were positively correlated with OLP clinical characteristics(P<0.05), CD19+(P<0.05) and IgA(P<0.05), respectively. Conclusion: Over expression of NGF and SP may be involved in the communication of nervous-immune system of OLP and promote the development of OLP.
引文
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[21] 中国中西医结合学会肾脏疾病专业委员会.IgA肾病西医诊断和中医辨证分型的实践指南[J].中国中西医结合杂志,2013,33(5):583-585.
[22] 肖雪野.抗CCP抗体、补体及免疫球蛋白在类风湿性关节炎病理过程中的变化及临床意义[J].海南医学院学报,2017,23(4):514-517.
[23] Divya VC,Sathasivasubramanian S.Estimation of serum and salivary immunoglobulin G and immunoglobulin A in oral pre- cancer:A study in oral submucous fibrosis and oral lichen planus[J].J Nat Sci Biol Med,2014,5(1):90-94.
[2] Matsumura S,Terao M,Murota H,et al.Th2 cytokines enhance TrkA expression,upregulate proliferation,and downregulate differentiation of keratinocytes[J].J Dermatol Sci,2015,78(3):215-223.
[3] Talbot S,Abdulnour RE,Burkett PR,et al.Silencing nociceptor neurons reduces allergic airway inflammation[J].Neuron,2015,87(2):341-354.
[4] Chiu IM,von Hehn CA,Woolf CJ.Neurogenic inflammation and the peripheral nervous system in host defense and immunopathology[J].Nat Neurosci,2012,15(8):1063-1067.
[5] Hayashi K,Karatsaidis A,Schreurs O,et al.NGF and its receptors TrkA and p75NTR in the epithelium of oral lichen[J].J Oral Pathol Med,2008,37(4):241-248.
[6] González Moles MA,Esteban F,Ruiz- Avila I,et al.A role for the substance P/NK- 1 receptor complex in cell proliferation and apoptosis in oral lichen planus[J].Oral Dis,2009,15(2):162-169.
[7] 朱立华,王建中.中国人血液淋巴细胞免疫表型参考值调查[J].中华检验医学杂志,1998,21(4):223-226.
[8] Eisen D,Carrozzo M,Bagan Sebastian JV,et al.Number V Oral lichen planus:Clinical features and management[J].Oral Dis,2005,11(6):338-349.
[9] Scully C,Carrozzo M.Oral mucosal disease:Lichen planus[J].Br J Oral Maxillofac Surg,2008,46(1):15-21.
[10] 王冬平,姜旺展,蔡扬,等.口腔扁平苔藓患者免疫功能状况与临床特征相关性分析[J].实用口腔医学杂志,2014,30(5):680-683.
[11] 龚非力.医学免疫学[M].4版.北京:科学出版社,2014:3.
[12] 黄韵颖,周塑,蔡扬.口腔扁平苔藓患者血清中白细胞介素- 12和白细胞介素- 27表达与免疫功能的相关性[J].华西口腔医学杂志,2016,34(2):140-144.
[13] Minnone G,De Benedetti F,Bracci- Laudiero L.NGF and Its Receptors in the Regulation of Inflammatory Response[J].Int J Mol Sci,2017,18(5)pii:E1028.
[14] Matsumura S,Terao M,Murota H,et al.Th2 cytokines enhance TrkA expression,upregulate proliferation,and downregulate differentiation of keratinocytes[J].J Dermatol Sci,2015,78(3):215-223.
[15] Talbot S,Abdulnour RE,Burkett PR,et al.Silencing Nociceptor Neurons Reduces Allergic Airway Inflammation[J].Neuron,2015,87(2):341-354.
[16] O' Connor TM,O'Connell J,O'Brien DI,et al.The role of substance P in inflammatory disease[J].J Cell Physiol,2004,201(2):167-180.
[17] Yu T,Calvo L,Anta B,et al.Regulation of trafficking of activated TrkA is critical for NGF- mediated functions[J].Traffic,2011,12(4):521-534.
[18] 钱悦,陈思远,黄长征,等.神经生长因子及其高亲和受体酪氨酸激酶、低亲和公共受体p75NTR在扁平苔藓皮损中的表达及意义[J].中华皮肤科杂志,2014,47(4):275-277.
[19] Karagouni EE,Dotsika EN,Sklavounou A.Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus[J].J Oral Pathol Med,1994,23(1):28-35.
[20] Snoeck V,Peters IR,Cox E.The IgA system:A comparison of structure and function in different species[J].Vet Res,2006,37(3):455-467.
[21] 中国中西医结合学会肾脏疾病专业委员会.IgA肾病西医诊断和中医辨证分型的实践指南[J].中国中西医结合杂志,2013,33(5):583-585.
[22] 肖雪野.抗CCP抗体、补体及免疫球蛋白在类风湿性关节炎病理过程中的变化及临床意义[J].海南医学院学报,2017,23(4):514-517.
[23] Divya VC,Sathasivasubramanian S.Estimation of serum and salivary immunoglobulin G and immunoglobulin A in oral pre- cancer:A study in oral submucous fibrosis and oral lichen planus[J].J Nat Sci Biol Med,2014,5(1):90-94.