柱前衍生高效液相色谱法测定石榴皮多糖的单糖组成
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摘要
建立了一种柱前衍生高效液相色谱法测定石榴皮多糖的单糖组成分析方法。石榴皮多糖经2. 0 mol/L的三氟乙酸水解后用1-苯基-3-甲基-5-吡唑啉酮(PMP)进行衍生化,采用Ultimate LP-C_(18)(250 mm×4. 6 mm,5μm)色谱柱,以乙腈-0. 05 mol/L pH 7. 0的NH4Ac水溶液(17:83,V/V)为流动相,在254 nm波长下检测。方法评价结果表明,本法测定单糖的线性范围宽、重复性好、准确性高,测得石榴皮多糖组分I和组分II中均含有甘露糖(Man)、鼠李糖(Rha)、葡萄糖醛酸(GlcA)、半乳糖醛酸(GalA)、葡萄糖(Glc)、半乳糖(Gal)和阿拉伯糖(Ara)等7种单糖。其摩尔比分别为0. 07∶0. 24∶0. 09∶1. 93∶0. 19∶1. 00∶3. 84和0. 09∶0. 67∶0. 20∶12. 98∶0. 39∶1. 00∶1. 76。该法能够快速实现石榴皮多糖样品乃至其他多糖样品的单糖组成分析。
High performance liquid chromatography( HPLC) was employed for the analysis of monosaccharide in pomegranate peel polysaccharides with pre-column derivatization. After polysaccharides were degraded by 2 mol/L trifluoracetic acid and then derivatized with 1-phenyl-3-methyl-5-prazolone( PMP),the HPLC was applied to analyze the PMP-monosaccharides derivatives with isometric elution using 17% acetonitrile-83% 0. 05 mol/L ammonium acetate( p H7. 0)solution as mobile phase and detection wavelength at 254 nm. The results showed that the established method for monosaccharides had wide linear range,good repeatability and high accuracy. Seven kinds of monosaccharides, such as mannose( Man), rhamnose( Rha), glucuronic acid( Glc A),galacononic acid( GalA),glucose( Glc),galactose( Gal) and arabinose( Ara),were discovered in the pomegranate peel polysaccharide I and II,and their molar ratios were 0. 07: 0. 24: 0. 09: 1. 93:0. 19: 1. 00: 3. 84 and 0. 09: 0. 67: 0. 20: 12. 98: 0. 39: 1. 00: 1. 76,respectively. The methodcan be used for rapid analysis of pomegranate peel polysaccharides and other polysaccharides.
High performance liquid chromatography( HPLC) was employed for the analysis of monosaccharide in pomegranate peel polysaccharides with pre-column derivatization. After polysaccharides were degraded by 2 mol/L trifluoracetic acid and then derivatized with 1-phenyl-3-methyl-5-prazolone( PMP),the HPLC was applied to analyze the PMP-monosaccharides derivatives with isometric elution using 17% acetonitrile-83% 0. 05 mol/L ammonium acetate( p H7. 0)solution as mobile phase and detection wavelength at 254 nm. The results showed that the established method for monosaccharides had wide linear range,good repeatability and high accuracy. Seven kinds of monosaccharides, such as mannose( Man), rhamnose( Rha), glucuronic acid( Glc A),galacononic acid( GalA),glucose( Glc),galactose( Gal) and arabinose( Ara),were discovered in the pomegranate peel polysaccharide I and II,and their molar ratios were 0. 07: 0. 24: 0. 09: 1. 93:0. 19: 1. 00: 3. 84 and 0. 09: 0. 67: 0. 20: 12. 98: 0. 39: 1. 00: 1. 76,respectively. The methodcan be used for rapid analysis of pomegranate peel polysaccharides and other polysaccharides.
引文
[1] Xie J H,Jin M L,Morris G A,Zha X Q,Chen H Q,Yi Y,Li J E,Wang Z J,Gao J,Nie S P,Shang P,Xie M Y. Crit.Eev. Food Sci.,2016,56(s):S60
[2] Shi X L,Yao C X,Lin X,Shen L,Feng Y. Chin. J. New Drug.,2014,23(9):1057时潇丽,姚春霞,林晓,沈岚,冯怡.中国新药杂志,2014,23(9):1057
[3] Venkataramanamma D,Aruna P,Singh R P. J Food Sci. Technol.,2016,53(5):2497
[4] Maihemuti M,Rebiyemu M,Tusunguli S,Aikebaier M. J. Med. Pharm. Chin. Minorities,2017,23(1):54买合木提·买买提,热比耶姆·毛拉托合提,吐逊古丽·撒塔尔,艾克白尔·买买提.中国民族医药杂志,2017,23(1):54
[5] Zhu C,Liu X. Carbohyd. Polym.,2013,92(2):1197
[6] Zhu C P,Zhai X C,Li L Q,Wu X X,Li B. Food Chem.,2015,177(6):139
[7] Zhang S F,Shi P P,Wang Q L,Shen J,Fu X. Food Sci,2008,29(6):280张书芬,史萍萍,王全林,沈坚,傅晓.食品科学,2008,29(6):280
[8] Ren H,Qiao H X,Fang L,Wang R W. Chin. J. Modern Appl. Pharm.,2015,32(6):711任红,乔洪翔,方玲,王如伟.中国现代应用药学,2015,32(6):711
[9] Yokota H,Mori K,Yamaguchi H,Kaniwa H,Saisho N. J Pharm Biomed Anal.,1999,21(4):767
[10] Ghfar A A,Wabaidur S M,Ahmed A Y,Alothman Z A,Khan M R,Al-Shaalan N H. Food Chem.,2015,176(6):487
[11] Sawardeker J S,Sloneker J,Jeanes Allene. Anal. Chem.,1965,37(12):1602
[12] Wang W Z,Chen F,Wang Y Q,Wang L N,Zheng F P,Beecher L. Food Chem.,2018,254(7):158-164
[13] Wang F Q,Zhang L,Lu Z Q,Wang Y Z. J Anal. Sci.,2016,34(1):32王凤芹,张林,路则庆,汪以真.分析科学学报,2016,34(1):32
[14] Liu Y,Li Q,Wang F,Chen X H,Sun L X,Bi K S. Chin. Drug Res. Pharmcol.,2011,22(2):200柳杨,李清,王菲,陈晓辉,孙应新,毕开顺.中药新药与临床药理,2011,22(2):200
[15] Chen Y W,Li Y J,Song M L,Cui Y T,Xu F X,Li J,Hu J H,Zhang Y Q. Contemp. Chem. Ind.,2017,46(8):1513陈燕文,李玉娟,宋梦璐,崔耀天,许方雪,李佳,胡晶红,张永清.当代化工,2017,46(8):1513
[2] Shi X L,Yao C X,Lin X,Shen L,Feng Y. Chin. J. New Drug.,2014,23(9):1057时潇丽,姚春霞,林晓,沈岚,冯怡.中国新药杂志,2014,23(9):1057
[3] Venkataramanamma D,Aruna P,Singh R P. J Food Sci. Technol.,2016,53(5):2497
[4] Maihemuti M,Rebiyemu M,Tusunguli S,Aikebaier M. J. Med. Pharm. Chin. Minorities,2017,23(1):54买合木提·买买提,热比耶姆·毛拉托合提,吐逊古丽·撒塔尔,艾克白尔·买买提.中国民族医药杂志,2017,23(1):54
[5] Zhu C,Liu X. Carbohyd. Polym.,2013,92(2):1197
[6] Zhu C P,Zhai X C,Li L Q,Wu X X,Li B. Food Chem.,2015,177(6):139
[7] Zhang S F,Shi P P,Wang Q L,Shen J,Fu X. Food Sci,2008,29(6):280张书芬,史萍萍,王全林,沈坚,傅晓.食品科学,2008,29(6):280
[8] Ren H,Qiao H X,Fang L,Wang R W. Chin. J. Modern Appl. Pharm.,2015,32(6):711任红,乔洪翔,方玲,王如伟.中国现代应用药学,2015,32(6):711
[9] Yokota H,Mori K,Yamaguchi H,Kaniwa H,Saisho N. J Pharm Biomed Anal.,1999,21(4):767
[10] Ghfar A A,Wabaidur S M,Ahmed A Y,Alothman Z A,Khan M R,Al-Shaalan N H. Food Chem.,2015,176(6):487
[11] Sawardeker J S,Sloneker J,Jeanes Allene. Anal. Chem.,1965,37(12):1602
[12] Wang W Z,Chen F,Wang Y Q,Wang L N,Zheng F P,Beecher L. Food Chem.,2018,254(7):158-164
[13] Wang F Q,Zhang L,Lu Z Q,Wang Y Z. J Anal. Sci.,2016,34(1):32王凤芹,张林,路则庆,汪以真.分析科学学报,2016,34(1):32
[14] Liu Y,Li Q,Wang F,Chen X H,Sun L X,Bi K S. Chin. Drug Res. Pharmcol.,2011,22(2):200柳杨,李清,王菲,陈晓辉,孙应新,毕开顺.中药新药与临床药理,2011,22(2):200
[15] Chen Y W,Li Y J,Song M L,Cui Y T,Xu F X,Li J,Hu J H,Zhang Y Q. Contemp. Chem. Ind.,2017,46(8):1513陈燕文,李玉娟,宋梦璐,崔耀天,许方雪,李佳,胡晶红,张永清.当代化工,2017,46(8):1513