绿薄荷茎和叶的乙醇提取物对小菜蛾拒食和产卵忌避活性的影响
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摘要
为有效控制小菜蛾的危害,明确绿薄荷对小菜蛾的防治效果,采用95%乙醇对绿薄荷茎和叶进行索式抽提,并测定了茎和叶提取物12.5、25.0和50.0 mg/mL浓度下小菜蛾幼虫的拒食率和成虫产卵忌避活性。结果显示,绿薄荷茎和叶的乙醇提取物对小菜蛾幼虫的拒食和成虫的产卵忌避均有显著作用,提取物浓度越高,拒食率和产卵忌避率越大。当茎和叶提取物浓度为12.5 mg/mL时,小菜蛾的拒食率最低,分别为36.16%和59.81%,产卵忌避率最低,分别为46.18%和57.78%;茎和叶提取物浓度为50.0 mg/mL时,拒食率最高,分别为82.67%和87.15%,产卵忌避率最高,分别为88.29%和98.27%。茎和叶对应的拒食中浓度(AFC_(50))最高分别为18.93 mg/mL和15.97 mg/mL,产卵忌避中浓度(AOC_(50))最高分别为16.38 mg/mL和15.03 mg/mL。
In order to control the damage of diamondback moth Plutella xylostella(L.) effectively,the new-style and highly active plant insecticides was researched. The Soxlet extraction method was used to extract the bioactive materials in the stems and leaves of the spearmint,Mentha spicata L. with 95%ethaonl,and the antifeedant activity on the 3rd instar larvae and oviposition deterrent activity on the adults of the diamondback moth at the extract concentrations of 12. 5,25. 0 and 50. 0 mg/mL were determined. The results indicated that the extracts of the spearmint stems and leaves showed significant antifeeding activity on the 3rd instar larvae and oviposition repellent effects on the adults of the diamondback moth. As the concentration of the exact increased,both antifeedant and antioviposition effects were enhanced. At the extract concentration of 12. 5 mg/mL of the stems and leaves,the lowest antifeedant rate were 36. 16% and 59. 81%,and the lowest antioviposition rate were 46. 18% and57. 78%,respectively. When the concentration was 50. 0 mg/mL,the highest antifeedant rate were82. 67% and 87. 15%,and the highest antioviposition rate were 88. 29% and 98. 27%,respectively.The highest median antifeedant concentrations( AFC_(50)) of the stems and leaves,were 18. 93 mg/mL and15. 97 mg/mL and the highest median antioviposition concentrations(AOC_(50)) of these were 16. 38 mg/mL and 15. 03 mg/mL,respectively.
In order to control the damage of diamondback moth Plutella xylostella(L.) effectively,the new-style and highly active plant insecticides was researched. The Soxlet extraction method was used to extract the bioactive materials in the stems and leaves of the spearmint,Mentha spicata L. with 95%ethaonl,and the antifeedant activity on the 3rd instar larvae and oviposition deterrent activity on the adults of the diamondback moth at the extract concentrations of 12. 5,25. 0 and 50. 0 mg/mL were determined. The results indicated that the extracts of the spearmint stems and leaves showed significant antifeeding activity on the 3rd instar larvae and oviposition repellent effects on the adults of the diamondback moth. As the concentration of the exact increased,both antifeedant and antioviposition effects were enhanced. At the extract concentration of 12. 5 mg/mL of the stems and leaves,the lowest antifeedant rate were 36. 16% and 59. 81%,and the lowest antioviposition rate were 46. 18% and57. 78%,respectively. When the concentration was 50. 0 mg/mL,the highest antifeedant rate were82. 67% and 87. 15%,and the highest antioviposition rate were 88. 29% and 98. 27%,respectively.The highest median antifeedant concentrations( AFC_(50)) of the stems and leaves,were 18. 93 mg/mL and15. 97 mg/mL and the highest median antioviposition concentrations(AOC_(50)) of these were 16. 38 mg/mL and 15. 03 mg/mL,respectively.
引文
Akhtar Y,Isman MB.2004.Comparative growth inhibitory and antifeedant effects of plant extracts and pure allelochemicals on four phytophagous insect species.Journal of Applied Entomology,128(1):32-38
Balasubramani V,Sayyed AH,Crickmore N.2008.Genetic characterization of resistance to deltamethrin in Plutella xylostella(Lepidoptera:Plutellidae)from India.Journal of Economic Entomology,101(6):1911-1918
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Choi WI,Lee EH,Choi BR,Park HM,Ahn YJ.2003.Toxicity of plant essential oils to Trialeurodes vaporariorum(Homoptera:Aleyrodidae).Journal of Economic Entomology,96(5):1479-1484
Furlong MJ,Wright DJ,Dosdall LM.2013.Diamondback moth ecology and management:problems,progress,and prospects.Annual Review of Entomology,58:517-541
Gao Y,Sheng GW,Shen JX,Xiao C.2012.Antifeeding activity of Vitex trifolia extracts against third-instar larvae of Plutella xylostella.Chinese Journal of Applied Entomology,49(5):1298-1303(in Chinese)[高燕,盛广为,沈嘉祥,肖春.2012.三叶蔓荆子提取物对小菜蛾的拒食活性.应用昆虫学报,49(5):1298-1303]
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Hou HM,Zhang X.2002.Effects of essential oil of plants on three lepidopterous insects antifeeding and growth inhibition.Journal of Plant Protection,29(3):223-228(in Chinese)[侯华民,张兴.2002.植物精油对三种鳞翅目害虫的杀虫活性.植物保护学报,29(3):223-228]
Hou YM,Pang XF,Liang GW.2002.Effects of azadirachtin against the diamondback moth,Plutella xylostella.Acta Entomologica Sinica,45(1):47-52(in Chinese)[侯有明,庞雄飞,梁广文.2002.印楝素乳油对小菜蛾种群的控制作用.昆虫学报,45(1):47-52]
Kumar P,Mishra S,Malik A,Satya S.2011.Insecticidal properties of Mentha species:a review.Industrial Crops and Products,34(1):802-817
Li JB,Fang LP,Xue JP,Chao FP,Guo Q,Zhang YN.2009.The bioactivity of ethanol extracts of Pinellia ternate against Plutella xylostella.Chinese Agricultural Science Bulletin,25(5):223-227(in Chinese)[李进步,方丽平,薛建平,晁飞朋,郭庆,张亚楠.2009.半夏乙醇提取物对小菜蛾幼虫生物活性的研究.中国农学通报,25(5):223-227]
Li SQ,Fang YL,Zhang ZN.2005.Studies and applications of botanical insect antifeedants.Chinese Journal of Applied Entomology,42(5):491-496(in Chinese)[李水清,方宇凌,张钟宁.2005.植物源昆虫拒食活性物质的研究和应用.昆虫知识,42(5):491-496]
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Li YK.1988.A preliminary study on use of the medical herb peppermint in control of cotton fusarium wilt.Scientia Agricultura Sinica,21(3):65-69(in Chinese)[李玉奎.1988.利用药用植物薄荷防治棉花枯萎病的初步研究.中国农业科学,21(3):65-69]
Liu SS,Li YH,Liu YQ,Zalucki MP.2005.Experience-induced preference for oviposition repellents derived from a non-host plant by a specialist herbivore.Ecology Letters,8(7):722-729
Lu Y.2007.Medicinal value and role of Mentha haplocalyx.Capital Medicine,14(16):44(in Chinese)[陆燕.2007.薄荷的药用价值及作用.首都医药,14(16):44]
LüJH,Liu SS.2006.Effects of Barbarea vulgaris on the development and oviposition preference of Plutella xylostella.Journal of Plant Protection,33(1):6-10(in Chinese)[吕建华,刘树生.2006.欧洲山芥对小菜蛾个体发育及产卵选择性的影响.植物保护学报,33(1):6-10]
LüJH,Liu SS.2007.The effects of volatiles from Barbarea vulgaris plants on choice response of Plutella xylostella female moths.Journal of Plant Protection,34(4):415-419(in Chinese)[吕建华,刘树生.2007.欧洲山芥植株挥发物对小菜蛾雌成虫选择行为的影响.植物保护学报,34(4):415-419]
Macharia I,Lhr B,Groote HD.2005.Assessing the potential impact of biological control of Plutella xylostella(diamondback moth)in cabbage production in Kenya.Crop Protection,24(11):981-989
Pant M,Dubey S,Patanjali PK.2016.Herbal insecticides,repellents and biomedicines:effectiveness and commercialization.New Delhi:Springer India,pp.117-126
Qin JD,Wang CZ.2001.The relation of interaction between insects and plants to evolution.Acta Entomologica Sinica,44(3):360-365(in Chinese)[钦俊德,王琛柱.2001.论昆虫与植物的相互作用和进化的关系.昆虫学报,44(3):360-365]
Rahman MM,Baker G,Powis KJ,Roush RT,Schmidt O.2010.Induction and transmission of tolerance to the synthetic pesticide emamectin benzoate in field and laboratory populations of diamondback moth.Journal of Economic Entomology,103(4):1347-1354
Rausher MD.2001.Co-evolution and plant resistance to natural enemies.Nature,411(6839):857-864
Ren JJ,Shi GL,Gu JC,Wang JW,Wang YN.2011.Effects of Mentha piperita extracts on activities of several enzymes of Tetranychus cinnabarinus.Scientia Silvae Sinicae,47(12):85-91(in Chinese)[任建军,师光禄,谷继成,王建文,王有年.2011.薄荷提取物对朱砂叶螨体内几种酶活性的影响.林业科学,47(12):85-91]
Schoonhoven LM,van Loon JJA,Dicke M.2005.Insect-plant biology.Oxford:Oxford University Press,pp.1-3
Tabashnik BE,Cushing NL,Finson N,Johnson MW.1990.Field development of resistance to Bacillus thuringiensis in diamondback moth(Lepidoptera:Plutellidae).Journal of Economic Entomology,83(5):1671-1676
Talekar NS,Shelton AM.1993.Biology,ecology,and management of the diamondback moth.Annual Review of Entomology,38(1):275-301
Wei H,Hou YM,Yang G,You MS.2004.Repellent and antifeedant effect of secondary metabolites of non-host plants on Plutella xylostella.Chinese Journal of Applied Ecology,15(3):473-476(in Chinese)[魏辉,侯有明,杨广,尤民生.2004.非嗜食植物次生物质对小菜蛾产卵驱避和拒食作用的研究.应用生态学报,15(3):473-476]
Xu HX,Yu XP,Chen JM,LüZX,Zheng XS,Han GX.2003.Effect of the crude extract from Tripterygium wilfordii on bioactivities of diamondback moth,Plutella xylostella L.Acta Agriculturae Zhejiangensis,15(2):83-86(in Chinese)[徐红星,俞晓平,陈建明,吕仲贤,郑许松,韩国兴.2003.雷公藤提取物对小菜蛾的生物活性.浙江农业学报,15(2):83-86]
Xu HX,Yu XP,LüZX,Chen JM,Zheng XS.2002.The effect of antifeedant and growth inhibition of crude extracts of Ginkgo biloba L.against the diamondback moth,Plutella xylostella L.Entomological Journal of East China,11(1):77-80(in Chinese)[徐红星,俞晓平,吕仲贤,陈建民,郑许松.2002.银杏叶粗提物对小菜蛾的拒避和生长发育抑制作用.华东昆虫学报,11(1):77-80]
Xu XR,Jiang HY,Zhang YN,Feng PZ.2006.Bioactivity of Pharbitis purpurea extracts against Plutella xylostella.Agrochemicals,45(2):125-126,128(in Chinese)[徐向荣,蒋红云,张燕宁,冯平章.2006.牵牛子提取物对小菜蛾的生物活性.农药,45(2):125-126,128]
Yang NW,Li AL.2007.Advances in the research of plant essential oils for pest control.Plant Protection,33(6):16-21(in Chinese)[杨念婉,李艾莲.2007.植物精油应用于害虫防治研究进展.植物保护,33(6):16-21]
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Balasubramani V,Sayyed AH,Crickmore N.2008.Genetic characterization of resistance to deltamethrin in Plutella xylostella(Lepidoptera:Plutellidae)from India.Journal of Economic Entomology,101(6):1911-1918
Chen L,Xu HH,Zhao SH.2000.Studies on antifeeding activity of Daphne tangutica against tobacco cutworm,Spodopetera litura.Journal of South China Agricultural University,21(1):44-46(in Chinese)[陈立,徐汉虹,赵善欢.2000.唐古特瑞香对斜纹夜蛾的拒食活性研究.华南农业大学学报,21(1):44-46]
Choi WI,Lee EH,Choi BR,Park HM,Ahn YJ.2003.Toxicity of plant essential oils to Trialeurodes vaporariorum(Homoptera:Aleyrodidae).Journal of Economic Entomology,96(5):1479-1484
Furlong MJ,Wright DJ,Dosdall LM.2013.Diamondback moth ecology and management:problems,progress,and prospects.Annual Review of Entomology,58:517-541
Gao Y,Sheng GW,Shen JX,Xiao C.2012.Antifeeding activity of Vitex trifolia extracts against third-instar larvae of Plutella xylostella.Chinese Journal of Applied Entomology,49(5):1298-1303(in Chinese)[高燕,盛广为,沈嘉祥,肖春.2012.三叶蔓荆子提取物对小菜蛾的拒食活性.应用昆虫学报,49(5):1298-1303]
Guo MC,Li BT,Tang LM,Li XH,Liang TJ.2015.Bioactivity of methanol extracts from Justicia procumbens against Plutella xylostella.Acta Ecologica Sinica,35(11):3854-3861(in Chinese)[郭明程,李保同,汤丽梅,李晓花,梁同军.2015.爵床的甲醇提取物对小菜蛾的生物活性作用.生态学报,35(11):3854-3861]
Hou HM,Zhang X.2002.Effects of essential oil of plants on three lepidopterous insects antifeeding and growth inhibition.Journal of Plant Protection,29(3):223-228(in Chinese)[侯华民,张兴.2002.植物精油对三种鳞翅目害虫的杀虫活性.植物保护学报,29(3):223-228]
Hou YM,Pang XF,Liang GW.2002.Effects of azadirachtin against the diamondback moth,Plutella xylostella.Acta Entomologica Sinica,45(1):47-52(in Chinese)[侯有明,庞雄飞,梁广文.2002.印楝素乳油对小菜蛾种群的控制作用.昆虫学报,45(1):47-52]
Kumar P,Mishra S,Malik A,Satya S.2011.Insecticidal properties of Mentha species:a review.Industrial Crops and Products,34(1):802-817
Li JB,Fang LP,Xue JP,Chao FP,Guo Q,Zhang YN.2009.The bioactivity of ethanol extracts of Pinellia ternate against Plutella xylostella.Chinese Agricultural Science Bulletin,25(5):223-227(in Chinese)[李进步,方丽平,薛建平,晁飞朋,郭庆,张亚楠.2009.半夏乙醇提取物对小菜蛾幼虫生物活性的研究.中国农学通报,25(5):223-227]
Li SQ,Fang YL,Zhang ZN.2005.Studies and applications of botanical insect antifeedants.Chinese Journal of Applied Entomology,42(5):491-496(in Chinese)[李水清,方宇凌,张钟宁.2005.植物源昆虫拒食活性物质的研究和应用.昆虫知识,42(5):491-496]
Li WP.2010.Insecticide resistance status and monitoring of diamondback moth(DBM)in Tianjin.Tianjin Agricultural Sciences,16(4):44-45(in Chinese)[李文萍.2010.天津市小菜蛾田间种群抗药性现状及监测.天津农业科学,16(4):44-45]
Li YK.1988.A preliminary study on use of the medical herb peppermint in control of cotton fusarium wilt.Scientia Agricultura Sinica,21(3):65-69(in Chinese)[李玉奎.1988.利用药用植物薄荷防治棉花枯萎病的初步研究.中国农业科学,21(3):65-69]
Liu SS,Li YH,Liu YQ,Zalucki MP.2005.Experience-induced preference for oviposition repellents derived from a non-host plant by a specialist herbivore.Ecology Letters,8(7):722-729
Lu Y.2007.Medicinal value and role of Mentha haplocalyx.Capital Medicine,14(16):44(in Chinese)[陆燕.2007.薄荷的药用价值及作用.首都医药,14(16):44]
LüJH,Liu SS.2006.Effects of Barbarea vulgaris on the development and oviposition preference of Plutella xylostella.Journal of Plant Protection,33(1):6-10(in Chinese)[吕建华,刘树生.2006.欧洲山芥对小菜蛾个体发育及产卵选择性的影响.植物保护学报,33(1):6-10]
LüJH,Liu SS.2007.The effects of volatiles from Barbarea vulgaris plants on choice response of Plutella xylostella female moths.Journal of Plant Protection,34(4):415-419(in Chinese)[吕建华,刘树生.2007.欧洲山芥植株挥发物对小菜蛾雌成虫选择行为的影响.植物保护学报,34(4):415-419]
Macharia I,Lhr B,Groote HD.2005.Assessing the potential impact of biological control of Plutella xylostella(diamondback moth)in cabbage production in Kenya.Crop Protection,24(11):981-989
Pant M,Dubey S,Patanjali PK.2016.Herbal insecticides,repellents and biomedicines:effectiveness and commercialization.New Delhi:Springer India,pp.117-126
Qin JD,Wang CZ.2001.The relation of interaction between insects and plants to evolution.Acta Entomologica Sinica,44(3):360-365(in Chinese)[钦俊德,王琛柱.2001.论昆虫与植物的相互作用和进化的关系.昆虫学报,44(3):360-365]
Rahman MM,Baker G,Powis KJ,Roush RT,Schmidt O.2010.Induction and transmission of tolerance to the synthetic pesticide emamectin benzoate in field and laboratory populations of diamondback moth.Journal of Economic Entomology,103(4):1347-1354
Rausher MD.2001.Co-evolution and plant resistance to natural enemies.Nature,411(6839):857-864
Ren JJ,Shi GL,Gu JC,Wang JW,Wang YN.2011.Effects of Mentha piperita extracts on activities of several enzymes of Tetranychus cinnabarinus.Scientia Silvae Sinicae,47(12):85-91(in Chinese)[任建军,师光禄,谷继成,王建文,王有年.2011.薄荷提取物对朱砂叶螨体内几种酶活性的影响.林业科学,47(12):85-91]
Schoonhoven LM,van Loon JJA,Dicke M.2005.Insect-plant biology.Oxford:Oxford University Press,pp.1-3
Tabashnik BE,Cushing NL,Finson N,Johnson MW.1990.Field development of resistance to Bacillus thuringiensis in diamondback moth(Lepidoptera:Plutellidae).Journal of Economic Entomology,83(5):1671-1676
Talekar NS,Shelton AM.1993.Biology,ecology,and management of the diamondback moth.Annual Review of Entomology,38(1):275-301
Wei H,Hou YM,Yang G,You MS.2004.Repellent and antifeedant effect of secondary metabolites of non-host plants on Plutella xylostella.Chinese Journal of Applied Ecology,15(3):473-476(in Chinese)[魏辉,侯有明,杨广,尤民生.2004.非嗜食植物次生物质对小菜蛾产卵驱避和拒食作用的研究.应用生态学报,15(3):473-476]
Xu HX,Yu XP,Chen JM,LüZX,Zheng XS,Han GX.2003.Effect of the crude extract from Tripterygium wilfordii on bioactivities of diamondback moth,Plutella xylostella L.Acta Agriculturae Zhejiangensis,15(2):83-86(in Chinese)[徐红星,俞晓平,陈建明,吕仲贤,郑许松,韩国兴.2003.雷公藤提取物对小菜蛾的生物活性.浙江农业学报,15(2):83-86]
Xu HX,Yu XP,LüZX,Chen JM,Zheng XS.2002.The effect of antifeedant and growth inhibition of crude extracts of Ginkgo biloba L.against the diamondback moth,Plutella xylostella L.Entomological Journal of East China,11(1):77-80(in Chinese)[徐红星,俞晓平,吕仲贤,陈建民,郑许松.2002.银杏叶粗提物对小菜蛾的拒避和生长发育抑制作用.华东昆虫学报,11(1):77-80]
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