摘要
为探索狮头柑籽油的提取工艺,给生产利用提供理论依据及技术方法。在优选了提取溶剂及单因素试验基础上,对料液比、提取时间、提取温度3因素运用中心组合(Box-Benhnken)试验设计进行狮头柑籽油提取技术研究。提取狮头柑籽油的适宜溶剂为乙醚;确定了狮头柑籽油索氏提取的优化工艺条件为:料液比1∶100(g/mL),提取温度49℃,提取时间7h,并拟合出狮头柑籽油得率的预测模型(R~2=0.985 9)。采用气相色谱-质谱联用法(gas chromatography-mass spectrometry,GC-MS)鉴定出狮头柑籽油中的脂肪酸组分,包括油酸、亚油酸、棕榈酸、花生酸等9种,其中亚油酸含量最高,达72.00%。研究提出的工艺技术可用于狮头柑籽油的提取生产,出油率可达37.61%。
This study aimed to explore the extraction technology of seed oil of Citrusreticulatacv.Manau Gan fruit(shitougan)to provide theoretical basis and technical methods for its production and utilization.Based on optimizing the extraction solvent and the result of single factor experiments,three factors,including solid-liquid ratio,extraction time,and extraction temperature were applied to the Box-Benhnken design principle.The results showed that diethyl ether was the suitable solvent for extracting shitougan seed oil,and the otimize process conditions of extraction shitougan seed oil were obtained as solid-liquid ratios:1∶100(g/mL),extraction temperature:49℃,extraction time:7 h.The prediction model for the yield of shitougan seed oil(R~2=0.985 9)was simulated.Fatty acid composition of shitougan seed oil was analyze by gas chromatography-mass spectrometry(GC-MS).Nine kinds of fatty acid component were identified,including oleic acid,linoleic acid,palmitic acid and arachidic acid,etc.The highest fatty acid component was linoleic acid(72.00%).The research and technology proposed could be used for the extraction and production of shitougan seed oil,with the oil yield of 37.61%.
引文
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