表面活性剂溴化十六烷基吡啶对产膜表皮葡萄球菌粘附作用的影响
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摘要
目的通过表面活性剂溴化十六烷基吡啶抗表皮葡萄球菌生物被膜菌粘附作用的研究,为临床针对产膜表皮葡萄球菌引起的相关感染提供可能的探索方向和防治途径。方法以万古霉素为阳性对照,利用XTT减低法,评价溴化十六烷基吡啶对表皮葡萄球菌初始粘附的影响。结果浓度为4、2和1 mg/L的溴化十六烷基吡啶对表皮葡萄球菌初始粘附均有显著的抑制作用,与阴性对照组比较差异有统计学意义(t=0.4555~34.9664,P<0.01);用浓度为32、16、8和4 mg/L的溴化十六烷基吡啶对实验材料预处理后,对表皮葡萄球菌初始粘附抑制作用明显,与阴性对照组比较差异有统计学意义(t=1.1125~21.9246,P<0.001)。结论溴化十六烷基吡啶无论是直接作用或是对实验材料预处理,对表皮葡萄球菌形成生物被膜的初始粘附过程具有显著抑制作用。
Objective To explore the influence of a surfactant,cetylpyridinium bromide,against the adhesion of biofilm-producing Staphylococcus epidermidis,and provide guidance for the research and treatment of the infections caused by the pathogen.Methods Vancomycin was used as the positive control.XTT reduction assay was used to evaluate the influence of cetylpyridinium bromide on the initial adhesion of Staphylococcus epidermidis.Results Cetylpyridinium bromide had significantly inhibitory effects on the initial adhesion of Staphylococcus epidermidis at the concentrations of 4 mg/L,2 mg/L and 1 mg/L respectively.Compared with the negative control group,the difference was statistically significant(t = 0.4555-34.9664,P < 0.01).Pretreatment of experimental materials with cetylpyridinium bromide at concentrations of 32 mg/L,16 mg/L,8 mg/L and 4 mg/L significantly inhibited the initial adhesion of Staphylococcus epidermidis compared with the negative control group(t = 1.1125-21.9246,P < 0.001).Conclusion Whether through direct action or pretreatment of experimental materials,cetylpyridinium bromide has significant inhibitory effects on the initial adhesion of Staphylococcus epidermidis in the process of producing biofilm.
Objective To explore the influence of a surfactant,cetylpyridinium bromide,against the adhesion of biofilm-producing Staphylococcus epidermidis,and provide guidance for the research and treatment of the infections caused by the pathogen.Methods Vancomycin was used as the positive control.XTT reduction assay was used to evaluate the influence of cetylpyridinium bromide on the initial adhesion of Staphylococcus epidermidis.Results Cetylpyridinium bromide had significantly inhibitory effects on the initial adhesion of Staphylococcus epidermidis at the concentrations of 4 mg/L,2 mg/L and 1 mg/L respectively.Compared with the negative control group,the difference was statistically significant(t = 0.4555-34.9664,P < 0.01).Pretreatment of experimental materials with cetylpyridinium bromide at concentrations of 32 mg/L,16 mg/L,8 mg/L and 4 mg/L significantly inhibited the initial adhesion of Staphylococcus epidermidis compared with the negative control group(t = 1.1125-21.9246,P < 0.001).Conclusion Whether through direct action or pretreatment of experimental materials,cetylpyridinium bromide has significant inhibitory effects on the initial adhesion of Staphylococcus epidermidis in the process of producing biofilm.
引文
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[2]Qin ZQ,Yang XM,Yang L,et al.Formation and properties of in vitro biofilms of ica-negative Staphylococcus epidermidis clinical isolates[J].J Med Microbiol,2007,56:83-93.
[3]JIA Ning,XU Zhikai,SUO Jijiang,et al.Biofilm production in Staphylococcus epidermidis isolated from nosocomial infection[J].Microbiol China,2006,33(3):88-91.(in Chinese)贾宁,徐志凯,索继江,等.引发医院感染表皮葡萄球菌生物被膜的检测[J].微生物学通报,2006,33(3):88-91.
[4]GUAN Yan,MA Yue,CHENG Huijuan,et al.Preliminary study of effects of matrine on Staphylococcus epidermidis biofilms[J].Microbiol China,2011,38(1):91-96.(in Chinese)官妍,马越,程惠娟,等.苦参碱对表皮葡萄球菌生物被膜作用初探[J].微生物学通报,2011,38(1):91-96.
[5]GUAN Yan,LI Chun,SHI Jingjin,et al.Effect of combination of sub-MIC sodium houttuyfonate and erythromycin on biofilm of Staphylococcus epidermidis[J].China J Chin Materia Medica,2013,38(5):731-735.(in Chinese)官妍,李春,石晶金,等.亚抑菌浓度鱼腥草素钠及与红霉素联合对表皮葡萄球菌生物被膜的作用[J].中国中药杂志,2013,38(5):731-735.
[6]GUAN Yan,XIE Meng,WANG Changzhong,et al.Inhibitory activity of Phillyrin and Baicalin against Staphylococcus epidermidis biofilm[J].Chin J Microecol,2010,22(10):886-893.(in Chinese)官妍,谢萌,汪长中,等.连翘苷和黄芩苷对表皮葡萄球菌生物膜抑制作用的研究[J].中国微生态学杂志,2010,22(10):886-893.
[7]YE Lianhua,HUANG Yunchao,YANG Dakuan,et al.Study on the structure of bacterial biofilm on PVC surface[J].Biomed Engin&Clin Med,2007,11(4):251-254.(in Chinese)叶联华,黄云超,杨达宽,等.聚氯乙烯材料表面细菌生物膜结构观察[J].生物医学工程与临床,2007,11(4):251-254.
[8]LU Xiaofeng,WU Jianxiang,XU Shaoyi,et al.Clinical evaluation of Vancomycin in the treatment of hospital-acquired methicillin-resistant Staphylococcus aureus pneumonia[J].Chin J Infect&Chemother,2015,15(3):214-216.(in Chinese)陆晓凤,吴建祥,徐少毅,等.万古霉素治疗医院获得性耐甲氧西林金黄色葡萄球菌肺炎的临床评价[J].中国感染与化疗杂志,2015,15(3):214-216.
[9]ZHANG Liang,HUANG Yunchao.Bacterial biofilm formation of Staphylococcus epidermidis and biomaterial infection[J].Biomed Engin&Clin Med,2006,10(3):194-196.(in Chinese)张良,黄云超.表皮葡萄球菌生物被膜形成与生物材料感染[J].生物医学工程与临床,2006,10(3):194-196.
[10]Qin Z,Qu Y,Yang L,et al.Role of autolysin mediated DNA release in biofilm formation of Staphylococcus epidermidis[J].Microbiology,2007,153(pt7):2083-2092.
[11]GUAN Yan,DONG Shizheng,SHI Jingjin,et al.A preliminary study on the influence of SDBS on Staphylococcus epidermidis adhesion in the process of producing biofilm[J].Chin J Microecol,2016,28(2):133-137.(in Chinese)官妍,董仕政,石晶金,等.十二烷基苯磺酸钠对产膜表皮葡萄球菌黏附作用影响初探[J].中国微生态学杂志,2016,28(2):133-137.
[12]WUJING Chemical Database[DB/OL].http://www.basechem.org/chemical/2506物竞化学品数据库[DB/OL].http://www.basechem.org/chemical/2506
[13]Stefańska J,Pietruczuk-Padzik A,Struga M,et al.Biological evaluation of quaternary bis ammonium salt and cetylpyridinum bromide against S.epidermidis biofilm[J].Pol J Microbiol,2013,62(4):359-364.